Lymphoma is one of the most common histological types of carcinoma of the bone marrow. The aim of the study was to determine the usefulness of the flow cytometry in diagnosing lymphomas on the basis of the material obtained from lymph nodes by the surgical biopsy in dogs. The material was lymph nodes obtained by surgical biopsy from 33 dogs suffering from multicentric lymphoma. The control group consisted of 4 clinically healthy dogs. The material was submitted to the flow cytometry analysis using the monoclonal antibodies and fluorochromes. The percentage of the CD4+ lymphocytes was lower in the affected animals than in healthy ones. The percentage of CD21-like + lymphocytes was higher in the affected animals than in healthy ones. The percentage of CD5+ and CD8+ was lower than in the healthy dogs. The research demonstrated that determining the fenotype of lymphocytes from lymph nodes in the case of multicentric lymphoma in dogs is an important addition to immunohistochemical methods.
We aimed at analysis of the clinical pattern and the course of lymphonodular toxoplasmosis taking into account the intensity and duration of the illness. Ninety seven patients (69 adults and 28 children) with lymphonodular toxoplasmosis were examined. Toxoplasmosis was diagnosed by indirect immunofluorescence; the titres of antibodies of IgG and IgM classes were also estimated. Depending on the duration of the illness, three periods: early, regression of symptoms and protraction of symptoms were defined; depending on the intensity of signs and symptoms of toxoplasmosis three courses of the disease: acute, subacute and chronic were defined. The analysis showed that clinical picture of lymphonodular toxoplasmosis was characterized by swelling of various peripheral lymph nodes as well as by general clinical symptoms (88 ,7% of cases) the intensity and duration of which, to a large extent, determined course of the illness. No correlation between clinical course and duration (early and regression period) of lymphonodular toxoplasmosis and titres IFA-IgG and IFA-IgM (before therapy) was found. Pathomorphology of lymph nodes in patients with toxoplasmic lymphadenopathy is a valuable complementary examination which renders it possible to determine the period of the disease.
In the course of experimental hymenolepidosis in rats, varying mobilization of mastocytes, dependent on the organ of their origin, was observed. The highest was in the small intestine, lower in the mesenterial lymph nodes and the spleen. Various degree of correlation between the mastocyte mobilization and the histopathological changes was stated.
The percentage of NBT-positive cells in peritoneal fluid, spleen and mesenteric lymph node of CWF mice infected orally with 200 Trichinella spiralis larvae was investigated. The highest level of NBT-positive cells in peritoneal fluid was found on the 5-6th and 50-60th day post infection (p.i.), in spleen between the 30-75th and in mesenteric lymph node between the 50-75th day p.i. The lack of reaction between peritoneal fluid cells and newborn migrating larvae at the 15th and 20th day p.i. observed by the authors, may be associated with slight activity of these cells in the NBT reduction test between 8-30th day p.i.