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Projectiles made of lead alloys are used for waterfowl hunting in Europe. The paper demonstrated that lead pellets, due to their construction and use, contaminate the environment, especially water ecosystems. During one hunting session, tens of thousands of lead balls are introduced to the environment. Moreover, dispersed heavy metal is ingested by birds as gastroliths; as a result, game birds as well as protected birds become intoxicated with lead. During hunting trips, birds are also injured. When entering into the food chain, lead from pellets poses a risk to many living organisms, including predators and scavengers. Injured or intoxicated birds have difficulty joining seasonal migrations. Meat, especially of wild ducks and geese, is consumed during the hunting season by hunters and their families. Considering the level of lead in the muscles of game birds, venison consumers are also exposed to lead intoxication. In Europe, an increasing number of hunters who use lead pellets has resulted in lead being accumulated in game birds, which is a hazard to the public health. In view of the above facts and other hazards resulting from hunting, which endanger humans and animals, the authors recommend a total ban on waterfowl hunting.
Most of the previous studies on the functional ultrastructure of oncospheral envelopes in cestodes are restricted to hymenolepidids with terrestrial life cycles, mainly parasites of mammals. The purpose of the present study is to describe and compare origin, differentiation and functional ultrastructure of oncospheral envelopes of 12 cestode species with aquatic life cycles examined by means of transmission (TEM) and scanning electron microscopy (SEM) techniques. Results of our comparative electron microscopical studies revealed that despite the general similarities in the ultrastructure of the primary envelopes surrounding developing embryos, there exist important differences both in the type of morphogenesis and in the final form and arrangement of the secondary envelopes between the 12 examined hymenolepidids. In all examined species, the embryophore develops within the syncytial layer of the primary inner envelope and is transformed into a heterogeneous structure in the final phase of infective egg formation. Some ultrastructural features of oncospheral envelopes are very characteristic for the cestode species examined, and may indeed represent a new useful criterion for differential diagnosis. Our data on ultramorphology of the envelopes and their connections with the uterine wall may also be useful for better understanding of the developmental physiology and biology of the oncospheral stage of hymenolepidids with aquatic life cycles. The comparative analysis allows determination of some ultrastructural features that adapt oncospheres to the behavior and habitat of their intermediate hosts. Interrelations among the ultrastructure of the oncospheral envelopes, habitat of crustacean intermediate hosts, and cestode life cycles are drawn and discussed.
In 1950s the Common Eider was a very rare species in the Black Sea region, migrating there only irregularly. A decade later, 9-14 males wintered in Black Sea bays. A few pairs attempted to breed there but their nests were flooded by storms. The first records (2 pairs) of successful breeding come from 1975. By the mid-1990s, the total number of breeding pairs had reached almost 1000.
Periods of severe winter weather are associated with increased food consumption and metabolic rates. Depending on food availability birds could use different strategies to maintain homeostasis. Mallards Anas platyrhynchos L. numerously winter in urban parks, where people feed waterfowl bread. This food source is easily digestible and provides a high energy, which may affect bird behaviour. Studies were conducted in two consecutive winters in the Gdańsk-Oliwa city park in northern Poland, a place where people feed mallards, bread daily. During the period of lowest temperatures (about -12°C) females spent only 6% of their time foraging, while males spent 17% foraging. During mild winter weather (mean temperature about -2°C) foraging took up 21% of time budget in both sexes. In the colder period a three fold increase in male agonistic behaviour was observed when compared to the mild period observations. Females spent the most of harsh winter period inactive, apparently relaying on accumulated energetic reserves. Males cannot reduce all activities like females, because pairing in mallards takes place mainly in autumn and early winter and males need to attract actively, display for, and defend mates.
The presented study aimed at application of the PCR methods for the detection and differentiation of goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV) in the liver, spleen, and faeces of infected geese and in litter from infected farms. For the improvement of specificity and efficiency of the methods, as well as for overcoming the frequent inhibition of PCR in samples extracted from faeces and litter, the "touchdown" thermal profile with additive of betaine were used. The isolation of the virus in goose embryo fibroblasts was used as a verifying method for GPV and MDPV detection. The presence of the cytopathic effect in infected cell cultures allowed for the detection of the both parvoviruses but not for their differentiation. As a result of this study, PCR methods for the fast detection of GPV and MDPV in field samples of visceral organs and faeces of infected geese and in their litter were developed.
It was proposed previously that passive dispersal by migratory aquatic birds explain the widespread distribution of many wetland organisms. Several experimental studies have shown that many widespread wetland plant species can be readily dispersed within the guts of Anatidae. However, it is unclear whether plants with a more restricted distribution are able to disperse via waterbirds. This paper addresses the dispersal ability and germination ecology of the little-known Hungarian milkvetch Astragalus contortuplicatus, which occurs on banks of continental rivers and has a limited and unpredictable distribution. To test whether limited capacity for endozoochory by waterfowl could explain the sporadic appearance of this species, we force-fed ten captive mallards (Anas platyrhynchos) with 100 milkvetch seeds each. Droppings were collected for up to 45 h after feeding. Intact and viable seeds were found in the droppings of each mallard, and altogether 24.7% of seeds fed were recovered intact. The proportion of retrieved seeds that germinated (27.0%) was significantly higher than that of untreated control seeds (0.5%), but significantly lower than that of mechanically scarified seeds (96.0%). Retrieved seeds that germinated developed into healthy mature plants. Given the average flight velocity of mallards, seeds of A. contortuplicatus may travel up to 1600 km inside the digestive tract of migrating individuals. Our results suggest that avian vectors may be more important for the dispersal of rare higher plants (especially those with a hard seed-coat) than hitherto considered. Moreover, they suggest that rarity does not necessarily indicate limited dispersal ability, and may instead be explained by specific habitat requirements.
Sixteen field strains of goose parvovirus (GPV) and DNA extracted from standard strain of Muscovy duck parvovirus (MDPV) were used. PCR was used for the amplification of the VP3 structural protein encoding region. In the case of all GPV strains and one MDPV strain the presence of specific product about 1,604 bp long was observed. The essential conditions, which had the influence on the specificity, sensitivity, and efficiency of the amplification reaction, were optimised. In order to eliminate unspecific interactions between template and primers touchdown PCR was applied. Additionally, for specificity and efficiency improvement, betaine (N, N, N - trimethylglycine) was added. The conducted optimisation steps of PCR allowed for the identification of the both parvoviruses.
A total of 47 avian faecal samples of wild waterfowl (great cormorant — Phalacrocorax carbo, great crested grebe — Podiceps cristatus, white stork — Ciconia ciconia) trapped in the eastern Slovakia were screened for the presence of human pathogenic microsporidia by microscopy and real-time SYBR Green PCR method using species primers and sequenced. Microscopic analysis showed presence in 32 samples (29 cormorants, 3 dippers). Microsporidial DNA (Encephalitozoon cuniculi genotype I) was identified in 19 faeces samples (40.4%) namely cormorants in 17 out of 40, one dipper of 5 and a stork out of 2. The present work describes three new host species of the bird population in microsporidium Encephalitozoon cuniculi genotype I which confirms the theory of low specificity of this species.
Over fifteen years (1985-1999) waterfowl were counted during one day in January on 33 permanent routes with a total length of c. 310 km along the banks of all rivers and ponds throughout the city. 23 species (apart from gulls) were recorded. The number of species varied from 3 to 10 in different years and increased significantly during the study period. Mallard Anas platyrhynchos was the most numerous species (98-99% of individuals). The proportion of male Mallards varied from year to year within the range 54—63%. The number of Mallards gradually increased from c. 17 300 in the winter of 1984-1985 to c. 28 000 in the winter of 1989-90 but then declined to c. 7500 in the winter of 1997-1998. These changes appear to depend on both air temperatures during the winter and the prosperity of the city's inhabitants, who feed ducks with bread and scraps of food. It is highly probable that the change in the socio-economic situation has been the main cause of the decline in waterfowl observed during the 1990s.
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