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Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
Pretreatment and analysis of urea and uric acid in body fluids and those of newly identified toxic compounds in dental material are described. Urea is a major uremic toxin. The accumulation of urea in blood may promote serious uremia syndrome if not regularly removed. Thus, an accurate analysis of blood urea is required for precise diagnosis. There have not been reported so far for differential analysis of free blood urea from bound urea as well as urea from endogenous ammonium. The author found the simplest differential method of urea and endogenous ammonium analysis in blood using a strong cation exchange resin column. For pretreatment of blood urea, solid phase extraction and dialysis were compared. The solid phase extraction method was found to be superior to dialysis in terms of recovery rate. Separation efficiency and reproducibility of blood urea determination using HPLC and MECC were also compared. Identification of newly toxic compounds in dental material, was carried out by LC-MS-MS and determination to saliva was by HPLC combined with SPE.
Corn was grown for green matter in a pot experiment, in which soil was fertilized with composts alone or in combination with 3 g N per pot (urea). The composts were made in wooden boxes, measuring 50×60×60 cm. They were composted for 3.5 months, until the temperature in the composts became stable and equal to the ambient temperature. Rates of the composts were balanced with amounts of added nitroge, such as 6.0 g N per pot. A one-factor experiment was conducted in Kick-Brauckmann pots, kept in a greenhouse at the University of Warmia and Mazury in Olsztyn. After harvest, fresh matter of stems and leaves as well as corn cobs was determined. Next, the plant samples were dried to determine the dry matter content and finally, after mineralisation, the concentration of Cu, Zn and Mn was determined by atomic absorption spectrophotometry. The composts significantly reduced the concentration of zinc and manganese in the vegetative yield of cor (lstems and leaves). Urea applied in combination with the composts very strongly increased the concentration of manganese and, to a lesser degree, the level of zinc and copper in vegetative organs. In corn cobs, the application of urea increased only the content of manganese. Urea had a stronger effect on increasing the weight of cobs rather than the vegetative mass of corn plants. The uptake of Cu, Zn and Mn was more evidently conditioned by the accumulation of these elements in dry matter than by the total weight of corn plants. The extent of the relationship between the uptake of Cu, Zn and Mn and their concentration of corn biomass is expressed the by corresponding correlation coefficients: 0.66, 0.65 and 0.68.
Although urea is the simplest N-containing organic compound ubiquitous in all aquatic environments, its role in N-nutrition of planktonic biota and relevance for eutrophication of freshwater ecosystems is still insufficiently defined and often bypassed. The dynamics of production of autochthonous urea as well as maximal potential net ureolytic activity (net URA Vmax) of phyto- and bacterioplakton were studied in mesocosm experiment and verified during the field studies conducted in the Great Mazurian Lake system (GMLS). Analysis of the obtained results revealed that the proteins were the main autochthonous urea precursors. Urea concentration in the studied mesocosms and in GMLS surface waters was positively correlated with flagellate, ciliate and crustacean biomass and, less evidently, with bacterial biomass (BB). In surface waters of GMLS net URA Vmax, similarly as urea concentrations, increased with their trophic status. Analysis of correlation of potential ureolytic activity with chlorophyllₐ, (Chlₐ) BB and L-leucine aminopeptidase activity (AMP) in lakes of different trophic status suggests that although both groups of planktonic microorganisms participated in urea decomposition processes, in eutrophic ones bacterial decomposition of urea is more evident. In highly eutrophic lakes excess of phosphorus induced higher nitrogen requirement resulting in the increase in protein decomposition rate. Intensified protein degradation resulted faster urea production, which finally induced higher ureolytic activity of planktonic microorganisms. In profundal waters of GMLS potential ureolytic activity was distinctly lower than in surface waters. This was caused by low temperature of hypolymnetic waters, inhibitory effect of hydrogen sulphide and lack of phytoplankton, which is known as a primary urea consumer.
Relationships between production traits and the parity of Polish Holstein-Friesian cows. The objectives of the study was to describe the relationships between milk urea, protein, fat concentrations and the age of Polish Holstein-Friesian cows. The experiment was carried out at the research dairy farm of the Warsaw University of Life Sciences (WULS). From a herd of 320 Polish Holstein Friesian cows maintained in a free–stall dairy shed 50 cows were selected. Parity had significant effect on the shaping the concentration of the production traits. The highest content of protein, 3.87%, was found in milk of cows during 3rd and subsequent lactations in the 10. collecting (above 271 day of lactation). The fat content ranged at the herd level from 3.18 to 4.89 %, the highest level has been recorded in milk of cows during 3th and subsequent lactations, in the 10. collecting (above 271 day of lactation). The urea content ranged at the herd level from 183 to 267 mg L -1. Milk production traits varies significantly with the age of cows. Hence, researchers should consider monitoring for this variable as potential cofounder when exploring the relationship between urea, protein, fat content in milk and nutritional management for meeting the production requirements of cows.
W trzyletnim doświadczeniu polowym badano wpływ dodatku inhibitora urolizy (PPDA) do mocznika na plonowanie i zawartość azotu w ziarnie i słomie oraz jego wykorzystanie z nawozów. Działanie mocznika modyfikowanego inhibitorem urolizy porównywano z mocznikiem tradycyjnym i saletrą amonową. Stwierdzono, że na plonowanie pszenicy jarej wywierał istotny wpływ jedynie poziom nawożenia azotem oraz sposób stosowania nawozów, natomiast nie udowodniono działania PPDA. Wykorzystanie azotu przez pszenicę z mocznika z dodatkiem PPDA wynosiło 70% i było o kilka procent wyższe niż z pozostałych nawozów.
The aromatic and curative properties of basil are connected with the presence of essential oil, mainly in the leaves and flowers. Herb yield and the quality of basil oil are related to the effect of genetic, ontogenetic and environmental factors. In the studies conducted in the years 2008-2009, the effect of basil cultivar and foliar feeding upon selected flowering features was examined. In an unheated plastic tunnel, plants of Kasia, Wala, Genua Star and Opal cultivars were grown. Foliar feeding was done by the application of 0.5% urea solution or by spraying the plants with water. During harvest the characteristic features of flowering were determined, i.e. the number and length of inflorescences, as well as the number of verticils and flowers per inflorescence. A vast differentiation was demonstrated in flowering of sweet basil plants of the examined cultivars. The most inflorescences were formed by the plants of the Polish cultivar Kasia, compared to the remaining ones. The longest inflorescences with the highest number of flowers in the main inflorescence were found in ‘Genua Star’. Foliar feeding of the plants with nitrogen caused a significant increase of inflorescence number, but it did not significantly affect the remaining features of basil flowering.
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