Wyniki wyszukiwania

1

The umbilical vein in man during the perinatal period

100%

Gorczyca J., Sokolowska-Pituchowa J., Skawina A.

Folia Morphologica

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1986

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tom 45

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nr 2

2

In vitro reoxygenation following hypoxia increases MMP-2 and TIMP-2 secretion by human umbilical vein endothelial cells

75%

Cavdar Z., Oktay G., Egrilmez M. Y., Genc S., Genc K., Altun Z., Islekel H., Guner G.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

69-73

Endothelial cells lining the inner blood vessel walls play a key role in the response to hypoxia, which is frequently encountered in clinical conditions such as myocardial infarction, renal ischemia and cerebral ischemia. In the present study we investigated the effects of hypoxia and hypoxia/reoxygenation on gelatinases (matrix metalloproteinase-2 and -9), their inhibitor (TIMP-2) and activator (MT1-MMP), in human umbilical vein endothelial (HUVE) cells. HUVE cells were subjected to 4 h of hypoxia or hypoxia followed by 4 and 24 h of reoxygenation. The pro- and active forms of MMP-2 and MMP-9 were analyzed by gelatin zymography; TIMP-2 protein level was assayed using ELISA, while MT1-MMP activity was measured using an activity assay. The secretion of MMP-2 proform increased significantly in cells subjected to 4h of hypoxia followed by 4 or 24 h of reoxygenation, compared with the normoxic group. TIMP-2 protein level also increased significantly in the hypoxia/reoxygenation groups, compared with the normoxic group. There were no statistically significant differences in the levels of active MT1-MMP in all groups. This study indicates that MMP-2 and TIMP-2 could be regarded as important components of a mechanism in the pathophysiology of ischemic injury following reperfusion.

3

Interleukin 1beta induces functional prostaglandin E synthase in cultured human umbilical vein endothelial cells

75%

Uracz W., Uracz D., Olszanecki R., Gryglewski R. J.

Journal of Physiology and Pharmacology

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2002

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tom 53

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nr 4,1

Prostaglandin endoperoxide H2 (PGH2) is generated from arachidonic acid by either constitutive (COX-1) or inducible (COX-2) cyclooxygenases. In arterial wall PGH2 is converted by PGI2 synthase (PGI-S) to prostacyclin (PGI2), and in platelets by thromboxane synthase (TX-S) to thromboxane (TXA2). Other prostanoids as PGD2, PGF2alpha or PGE2 were believed to arise non-enzymatically from PGH2. Only recently, human prostaglandin E synthase (PGE-S) has been identified and cloned as a membrane bound, microsomal, glutathione-dependent inducible enzyme. Here we demonstrated that interleukin 1ß (IL-1ß) is an inducer of COX-2 and PGE-S in human umbilical vein endothelial cells (HUVEC). Functional expression of PGE-S was measured at the level of specific mRNA by semi-quantitative RT-PCR, PGE-S protein was detected by Western blot in HUVEC, while PGE2 was measured by immunoassay in the supernatant. Actinomycin D, a classical transcription inhibitor, was used to prove that indeed IL-1ß induced the functional PGE-S enzyme. PGE2 generation in HUVEC was inhibited by indomethacin, acetamoniphen and dexamethasone. In conclusion, we found that in cultured endothelial cells IL-1ß induced as evidenced by the appearance of its transcript and its functional enzyme. The induction of endothelial PGE-S and COX-2 appeared to be and their transcripts were induced as fast as one might expect from immediate early genes. It means that IL-1ß-triggered-PGE2 biosynthesis in endothelial cells is probably regulated by induction of both COX-2 and PGE-S. This is way we hypothesise the existence of at least two distinct pools of COX-2: the first selectively coupled to PGE-S and the second one that is coupled to PGI-S yielding the main endothelial product - PGI2.

4

Isoprenoid depletion by statins antagonizes cytokine-induced down-regulation of endothelial nitric oxide expression and increases NO synthase activity in human umbilical vein endothelial cells

75%

Jantzen F., Konemann S., Wolff B., Barth S., Staudt A., Kroemer H. K., Dahm J. B., Felix S. B., Landsberger M.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 3

Endothelial dysfunction and atherosclerosis are associated with an inflammation-induced decrease in endothelial nitric oxide synthase (eNOS) expression. Based on the differences between hydrophobic and hydrophilic statins in their reduction of cardiac events, we analyzed the effects of rosuvastatin and cerivastatin on eNOS and inducible NO synthase (iNOS) expression and NOS activity in TNF-alpha-stimulated human umbilical vein endothelial cells (HUVEC). Both statins reversed down-regulation of eNOS mRNA and protein expression by inhibiting HMG-CoA reductase and isoprenoid synthesis. Cerivastatin tended to a more pronounced effect on eNOS expression compared to rosuvastatin. NOS activity - measured by conversion of [3H]-L-arginine to [3H]-L-citrulline - was enhanced under treatment with both drugs due to inhibition of HMG-CoA reductase. Statin-treatment reduced iNOS mRNA expression under normal conditions, but had no relevant effects on iNOS mRNA expression in cytokine-treated cells. Rosuvastatin and cerivastatin reverse the detrimental effects of TNF-alpha-induced down-regulation in eNOS protein expression and increase NO synthase activity by inhibiting HMG-CoA reductase and subsequent blocking of isoprenoid synthesis. These results provide evidence that statins have beneficial effects by increasing eNOS expression and activity during the atherosclerotic process.

5

Doppler study of the peripheral flows in early gestation

63%

Wloch A., Sodowski K., Rozmus-Warcholinska W., Wloch S., Bodzek P., Czuba B., Borowski D., Cnota W., Kuka D., Szaflik K., Huhta J.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 4

77-85

6

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

63%

Alrefaei G. I., Ayuob N. N., Ali S. S., Al-Karim S.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 3

7

Comparison of the functional activities of two different preparations of human recombinant tumor necrosis factor alpha

63%

Zeman K., Paleolog E. M., Tchorzewski H., Szymanska B.

Archivum Immunologiae et Therapiae Experimentalis

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1993

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tom 41

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nr 2

8

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Thienopyridines: effects on cultured endothelial cells

63%

Ziemianin B., Olszanecki R., Uracz W., Marcinkiewicz E., Gryglewski R. J.

Journal of Physiology and Pharmacology

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1999

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tom 50

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nr 4

Ograniczanie wyników

The umbilical vein in man during the perinatal period

In vitro reoxygenation following hypoxia increases MMP-2 and TIMP-2 secretion by human umbilical vein endothelial cells

Interleukin 1beta induces functional prostaglandin E synthase in cultured human umbilical vein endothelial cells

Isoprenoid depletion by statins antagonizes cytokine-induced down-regulation of endothelial nitric oxide expression and increases NO synthase activity in human umbilical vein endothelial cells

Doppler study of the peripheral flows in early gestation

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Comparison of the functional activities of two different preparations of human recombinant tumor necrosis factor alpha

Thienopyridines: effects on cultured endothelial cells