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Toxicity of salinomycin and narasin in turkeys

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Coccidiosis continues to be one of the most serious diseases in poultry breeding, causing major economic losses in this industry. Many trials are conducted to prevent and control this disease. However, only a few medications are approved for use in the prevention and treatment of coccidiosis. Ionophore coccidiostats (e.g. salinomycin and narasin) act by altering the transmembrane movement of monovalent or divalent ions (Na+, K+, Ca2+, Mg2+, Rb+, Cs+), resulting in altered ionic gradients and disturbed physiological processes in coccidia. In broiler and turkey breeding, these agents are used for nearly the whole fattening period. Ionophore coccidiostats have a narrow safety margin. Their toxicity is probably due to a disturbed ion balance or to oxidative damage. Although ionophore coccidiostats are considered to be relatively safe for target animals, there are numerous reports of poisoning cases caused by these medications in birds. This paper summarizes the current state of knowledge on the toxicity of salinomycin and narasin in turkeys. It reviews the data concerning the symptoms, mortality rate and possible causes of poisoning with these agents. Moreover, the paper discusses the legal regulations regarding the use of these drugs in poultry.
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The objective of this study was to determine whether silica grit is a necessary dietary additive for turkeys raised in intensive production farms, and whether it can be replaced by other supplements such as charcoal or hardwood ash. The conclusions were formulated based on performance results, blood hematological and biochemical indices, the slaughter quality of turkeys, as well as on the chemical and physicochemical properties of turkey meat. The experimental material comprised 360 male Big 6 heavy-type turkeys randomly divided into 4 treatments and raised on litter until 20 weeks of age. Birds of all treatments were fed identical complete pelleted basal diets. The experimental factor were various feed supplements in each group. The control treatment was fed a diet without supplements. The diets for experimental groups were supplemented with silica grit (SG), charcoal (CH) or hardwood ash (HA) in the amount of 0.3% of the administered feed mix. Dietary supplements were administered from the first day of life until the end of the rearing period. No significant changes in blood hematological and biochemical indices of turkeys were observed. Silica grit (SG) had an adverse effect on the performance results of turkeys, while the addition of both CH and HA had a highly beneficial impact. The best results were reported in respect of charcoal. The treatment fed a diet supplemented with CH was characterized by the lowest mortality rate of 4.4%, i.e. half that observed in the control group, body weight higher by 3.9% and rearing efficiency index higher by 9.7%.
This review article presents fundamental mechanisms of the local mucosal immunity in selected regions of the respiratory tract in healthy birds and in some pathological conditions. The respiratory system, whose mucosa come into direct contact with microorganisms contaminating inhaled air, has some associated structures, such as Harderian gland (HG), conjunctive-associated lymphoid tissue (CALT) and paranasal glands (PG), whose participation in local mechanisms of the mucosal immunity has been corroborated by numerous scientific studies. The nasal mucosa, with structured clusters of lymphoid tissue (NALT – nasal-associated lymphoid tissue) is the first to come into contact with microorganisms which contaminate inhaled air. Lymphoid nodules, made up of B cells with frequently developed germinal centres (GC), surrounded by a coat of CD4+ cells, are the major NALT structures in chickens, whereas CD8+ cells are situated in the epithelium and in the lamina propria of the nasal cavity mucosa. Studies into respiratory system infections (e.g. Mycoplasma gallisepticum) have shown the reactivity of the tracheal mucosa to infection, despite a lack of essential lymphoid tissue. Bronchus-associated lymphoid tissue (BALT) takes part in bronchial immune processes and its structure, topography and ability to perform defensive function in birds is largely age-dependent. Mature BALT is covered by a delicate layer of epithelial cells, called follicle-associated epithelium (FAE). Germinal centres (GC), surrounded by CD4+ cells are developed in most mature BALT nodules, while CD8+ lymphocytes are dispersed among lymphoid nodules and in the epithelium, and they are rarely present in GC. Macrophages make up the first line of defence mechanisms through which the host rapidly responds to microorganisms and their products in the respiratory mucosal system. Another very important element are polymorphonuclear cells, with heterophils being the most important of them. Phagocytic cells obtained from lung lavages in birds are referred to as FARM (free avian respiratory macrophages). Their number in chickens and turkeys is estimated to be 20 times lower than that in mice and rats, which indicates a deficit in the first-line of defence in the birds’ respiratory system. There are numerous B cells and antibody secreting cells (ASC) present throughout the respiratory system in birds. Their role comes down to perform antigen-specific protection by producing antibodies (IgM, IgY or IgA class) as a result of contact with pathogenic factors.
Research has shown that among pesticides, carbaryl is one of the strongest immunosuppressors for numerous animal species. In this paper, the effect of carbaryl on biochemical parameters and on specific and non-specific immunity in carbaryl-intoxicated birds was determined. The results indicate a significant immunosuppressive effect in turkeys, on almost all the investigated biochemical parameters and on the indices of specific and non-specific immunity. The only exception is a slightly elevated level of total protein and reactivity of T lymphocytes to non-specific mitogen in the group of intoxicated birds.
The study was carried out on 4200 turkey broilers Big 6, from which after a 3-week breeding an experimental group was separated. The group was receiving an addition of bioplex-Cu 0.5g/L of drinking water till the end of the experiment. The haematological and biochemical factors were examined in the 8th week of the turkeys life, in blood taken from the wing vein of 10 randomly chosen birds (5 cocks and 5 hens) from both experimental and control group. In the obtained samples, Ht, total protein level, a-NH2 nitrogen, glucose, and the Cu, Fe, Zn and Ca level were determined. Also, in the bioplex-Cu and in the feeds used, Cu, Fe, Zn and Ca were determined by the spectrophotometry of atom absorption method after dry mineralization. The applied bioplex-Cu statistically significantly increased the haematocrit and glucose level. The addition of Cu also caused an increase in the concentration of this element in the plasma of turkey blood. The significantly increased Cu level did not cause interaction with other components of the feed and did not disturb the Fe, Zn and Ca absorption to the turkey blood.
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