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It has been found that tegument homogenate of Ascaris lumhricoides suis and also trypsin inhibitor isolated from it induce the Leghorn chick embryos mortality when injected into their yolk sac on 4th, 8th or 13th day of incubation. The trypsin inhibitor is one of important components of Ascaris homogenate causing mortality. There is linear interrelationship between the logarithm of dose of homogenate or trypsin inhibitor and the mortality of chickens in %. A significant decrease of mean mass of chicks injected with Ascaris homogenate or trypsin inhibitor in comparison with control groups was observed. There was more frequent occurrence of developmental abnormalities and pathological changes in groups of hatched chicks which received Ascaris homogenate or inhibitor.
The studies were carried out on guinea pig males weighting about 280 g. The animals were infected with 5000 or 20000 invasive eggs of Ascaris suum. On the 3-rd, 7-th, 10-th and 14-th day of invasion the animals were dissected. Lungs were weighted, and the number of Ascaris larvaes was estimated using Baermann method. The activity of alpha-amylase (Fennel's method) and trypsin (Anson's method) were examined in pancreas homogenate. The infection of guinea pigs with A. suum larvaes resulted in a decrease of amylase and trypsin activity in parcreas. The activities were the lowest at the 3-rd day of invasion for amylase and at the 7-th day for trypsin. The results obtained for the animals infected 5 or 20000 invasie eggs A. suum did not differ statically.
The studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. The activity of trypsin was determined according to the method of Anson, and amylase of Fennel method. The measurements of activity were done at the presence of homogenized invasive eggs of Ascaris suum, and the mixture of volatile organic acids C₂-C₆ (at concentrations of 30.3 and 0.3 mM/l). In samples with homogenized invasive eggs of A. suum the amylase activity remained unchanged while the activity of trypsin was considerably higher (p < 0,01). The introduction of the organic acids at conc. 30 mM/l into the samples was the reason of lower amylase activity. No influence of these acids on trypsin activity in pancreas extracts was observed.
Trawienie pepsyną ekstraktów nasion spożywanych przez człowieka powoduje obniżenie aktywności trypsyny i chymotrypsyny.
The activity of alpha-amylase in the experimental groups of guinea pigs was lower than in control animals. The decreased level of activity of trypsin was noted (p<0.05) also in infected animals, and in treatment with organic acids. In the group of guinea pigs, which was administrated homogenized eggs of parasite, the activity of enzyme was a little higher than in control animals. The infection of animals was associated with increase of relative weight, of lungs. The guinea pigs treatment with organic acids had also a higher weight of spleen.
The guinea pigs were infected orally or intraintestinally (after laparotomy) with 5000 invasive eggs of Ascaris suum. On the 7th day after infection the invasion was controlled by lung's weight, degree of congestion, and number of larvae in the lungs. The activity of amylase was determined according to Fennel method, trypsin to Anson method. The activities of both enzymes in pancreas of orally infected animals (after laparotomy or not) were lower, and relative weight of lungs of these animals was higher than in control animals. In pancreas of guinea pig with invasive material introduced into its, the activities of amylase and trypsin were higher than in control animals, but only the activity of trypsin differed significantly.
W pracy określono wpływ obecności i stężenia jonów Pb (II) i Cd (II) oraz stężenia substratu na aktywność pepsyny i trypsyny w warunkach in vitro. Stwierdzono znaczący wzrost aktywności pepsyny ze wzrostem stężenia jonów tych metali w układzie reakcyjnym, natomiast duży nadmiar substratu w stosunku do enzymu obniżał aktywność obu enzymów.
Analizowano właściwości funkcjonalne handlowego izolatu białek grochu w porównaniu z produktami białkowymi uzyskanymi po łagodnej hydrolizie trypsyną. Odnotowano wyraźny wpływ p H na badane właściwości analizowane przy pH 7 oraz pH 8.
The study was carried out on Astra S chickens which were grown on diets containing 11% and 19% proteins. In homogenized pancreas and duodenal contents from control animals and chickens infected with 500 invasive eggs of Ascaridia galli activities of alpha-amylase (FENNEL method), lipase (CHERRY-CRANDALL method) and trypsin (ANSON method) were determined. After 7 weeks of the invasion the activities of these enzymes were higher in duodenal contents and lower in pancreas of infected birds in comparison with the control animals. The differences were significant for alpha-amylase and lipase activities in animals which were given 11% protein diet, and for trypsin activity of chickens groups fed with 19% protein diet.
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