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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Hairy root cultures of marigold (Calendula officinalis ) as a source of oleanolic acid glycosides

100%
Markowski M., Alsoufi A. S. M., Paczkowski C., Szakiel A., Dlugosz M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
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nr 1
2

Antifungal activity of triterpenoid isolated from Azima tetracantha leaves

100%
Duraipandiyan V., Gnanasekar M., Ignacimuthu S.
Folia Histochemica et Cytobiologica
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2010
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tom 48
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nr 2
311-313
3

Secondary metabolites in cell culture of clary sage [Salvia sclarea L.]

84%
Kuzma L., Skrzypek Z., Wysokinska H.
Herba Polonica
|
2004
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tom 50
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nr 3-4
Suspension culture was established from the roots of Salvia sclarea L. The maximum suspension cell growth was 14.3 g/flasl< (fresh mass) and 0.9 g/flasl< (dry mass), on the 18"' and 15"' day after inoculation, respectively. The isolation and characterisation of abietane type diterpenoids and phenolics, including flavones and rosmarinic acid from the cell culture was reported. A GC-MS analysis also showed the presence of pentacyclic triterpenoids and sterols in in vitro cultured cells of S. sclarea.
4

Biosynthesis of oleanolic acid glycosides in protoplasts isolated from Calendula officinalis L. roots

84%
Ruszkowski D., Szakiel E., Auguscinska E., Janiszowska W.
Acta Physiologiae Plantarum
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2006
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tom 28
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nr 3
Many medic inal plants contain oleanane saponins in roots, however, only scarce data on their biosynthesis in this organ are available so far, including our previous results concerning Calendula officinalis plant. Thus, the purpose of the present work was to confirm the presumable biosynthetic pathway of oleanolic acid glycosides in roots of young C. officinalis plants. First of all, the effective method of isolation of protoplasts from C. officinalis roots was established. Then, isolated root protoplasts were supplied with radioactive precursors, [2-¹⁴C] mevalonate (MV A) and [3-³H] oleanolic acid (OL) and their transformations were studied with comparison to results obtained with excised roots. The penetration of both precursors into protoplasts was more rapid and effective than in the case of excised roots. The labeling of sterols and OL during the incubation with MVA showed that the isoprenoid pathway leading to triterpenoids was operative in excised roots as well as isolated root protoplasts. Moreover, the transformations of OL into two seties of its glycosides, i.e. glucosides and glucuronides were investigated. It has been shown that both series of OL glycosides are synthesized in isolated root protoplasts in the same way as in excised roots of young marigold plants.
5
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Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach

67%
Kikowska M., Wlodarczyk A., Stochmal A., Zuchowski J., Thiem B.
Herba Polonica
|
2019
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tom 65
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nr 1
Introduction: Callus and cell suspension cultures are widely applied in investigation of production of highvalue secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance. Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary metabolites – pentacyclic triterpenoids and polyphenols. Methods: To establish a cell suspension culture, stabilized and homogeneous callus was selected. Cell cultures were systematically passaged every 2 weeks to fresh liquid medium with the same composition. Biomass from cultures at the growth phase and stationary phase was designated for phytochemical research. UHPLC-DAD-MS analyzes were performed. At the same time, their macroscopic and microscopic observations were carried out. Results: Cells of suspension culture line A2 were characterized by the intense divisions. Cell culture extracts (both from the growth phase and stationary phase) contained pentacyclic triterpenoids. In addition, phenolic compounds (chlorogenic acid and proanthocyanidins type B) and in a small amount also epicatechin are present in the extract of the cells harvested from the growth phase. In the present studies, three pentacyclic triterpenoids were detected and quantified in the extracts of cell suspensions and callus line A2. Ursolic and oleanolic acids were the main triterpenoids in the studied extracts. The cell suspension culture from the growth phase exhibited the highest content of ursolic, oleanolic, and betulinic acid (separately and together). Conclusion: The cell suspension culture of Chaenomeles japonica is a promising source of pentacyclic triterpenoids.
6

Distribution of triterpene acids and their derivatives in organs of cow-berry [Vaccinium vitis-idaea L.] plant

67%
Szakiel A., Mroczek A.
Acta Biochimica Polonica
|
2007
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tom 54
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nr 4
733-740
Wild berries of the genus Vaccinium have become increasingly popular in human health promotion due to their nutritional and medicinal properties. Some striking divergence of opinion about the content of triterpenoids in these plants still exists, meanwhile, this very large class of natural isoprenoids exhibits a wide range of biological activities and hence is of growing research interest. An investigation of triterpenoidal constituents from the cowberry (Vaccinium vitis-idaeaL.) plant led to the isolation of two isomeric acids:oleanolic and ursolic and the occurrence of their derivatives in this plant was demonstrated for the first time. Free triterpene acids as well as small amounts of their bound forms (presumable glycosides and glycoside esters) occur in fruits and the vegetative part of the plant, however, in various amounts and different ratios. The total content of both acids was the highest in organs regarded as traditional herbal resources, namely fruits and leaves (1 and 0.6% of dry mass, respectively), whereas it was markedly lower in stems and rhizomes. However, the rhizomes were in turn the plant organ containing relatively the highest amount of the bound forms of both acids (0.01% of dry mass). Ursolic acid was dominant in the whole plant, but the ratio of oleanolic to ursolic acid was significantly different in individual organs, decreasing from the upper (fruits 1:2.4, leaves 1:2) to the lower (stems 1:3.5, rhizomes 1:5.2) parts of the plant. This pattern of distribution of triterpenoids in the plant may have an important physiological and ecological meaning.
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