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Background: The aim of the present study was assessing the possibility of experimental allogenic transplantation of cat cornea endothelial cells, multiplied in vitro, into the anterior chamber of the eyeball in recipient cats. The reason for undertaking the research is the need to develop a method that would help in the cornea treatment in animals with corneal opacification following cataract surgery, as well as lens dislocation, injuries and endothelium degeneration. Methods: Cats aged 10-12 months were used in the experiment. Cornea fragments consisting of the posterior limiting membrane and posterior epithelium were placed in Iscove;s medium with addition of 10% foetal calf serum. Multiplied in vitro cells were injected into the anterior chamber of recipient cats. The cornea was subject to histological, histometric and SEM examination on the 3rd, 7th, 20th and 30th day after the surgery. Results: Micromorphological examination of the cornea showed full restitution of its endothelium 30 days after transplantation. Complete regeneration of structures indispensable for normal functioning of the posterior epithelium occurred as a result of implantation. Conclusions: In this study the results show that implantation of the cells of posterior corneal epithelium of donor cats, multiplied into vitro and injected into the anterior chamber of recipient cats. The cornea regained its full function, the layer of the posterior epithelium was regenerated and the stroma stabilized, presenting the image of full and proper corneal translucency.
Objective: There is increasing evidence that the transplanted bone marrow stromal cells (BMSC) significantly promote functional recovery after central nervous system damage in the animal models of various kinds of CNS disorders, including cerebral infarct. However, there are several shortages of information when considering clinical application of BMSC transplantation for patients with neurological disorders. In this meeting, therefore, we discuss what we should clarify to establish cell transplantation therapy in clinical situation and describe our recent works for this purpose. Methods and Results: The BMSC have multiple abilities to differentiate into the neural cells and to promote neuronal survival and axon elongation, contributing to rebuild the neural circuits in the injured CNS. Using optical imaging and MRI techniques, the transplanted BMSC can non-invasively be tracked in the living animals for at least 8 weeks after transplantation. Clinical MR apparatus can visualize the tagged BMSC in the brain. FDG PET is quite valuable to monitor the recovery of brain metabolism after transplantation. The BMSC can be expanded using the animal protein-free culture medium within a clinically relevant period. G-CSF is useful to enhance their proliferation when the BMSC are obtained from the aged patients. There are optimal dose and timing of BMSC transplantation to yield significant therapeutic benefits. Conclusion: It is urgent issues to develop clinical imaging technique to track the transplanted cells in the CNS and evaluate the therapeutic significance of BMSC transplantation to establish it as a definite therapeutic strategy in clinical situation in very near future.
The results of transplantations of organs and tissues in our country are comparable to these obtained in the best centres in the world. Only the number of the performed operations is significantly smaller than the actual needs. The aim of the study was having knowledge and stance about transplantations and judging knowledge about valid in Poland law rules about drawing and transplanting organs, tissues and cells in research community. On the basis of carried out researches it was certified, that the knowledge about transplantation organs is high. For most people the ascertainment of death brain is the same like death body. The necessary is to make training for future doctors where they can learn to make decisions about transplantations organs from death people and where they can learn to explain any problems of ethic-law nature of transplantation."
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