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The present study was aimed to develop a cost-effective and efficient protocol for mass propagation of high-quality seedlings through tissue culture by using seaweed extract as biostimulants instead of synthetic chemicals. The nodal explant of field grown W. somnifera estabilised on Murashige and Skoogs medium (MS) and Gamborg B5 medium supplemented with six concentration of 2,4 D. The percentage of culture response from the nodal explant ranged from 44 to 80 and 3.0 mg l-1 2,4 D found to be best for callus induction. MS media containing different concentration of seaweed extract (10, 20, 40, 60, 80 and 100 %) were tested individually for shoot induction. The medium supplemented with 40 % seaweed extract exhibited maximum number of shoots with about 8.6 shoots/ callus and 80 % seaweed extract exhibited 4.3 shoots/ callus. It is evident from this study that seaweed extracts can be used as substitute for synthetic growth hormones for micropropagation of medicinally important plant W. somnifera for clonal propagation and conservation.
The leaves of Eucalyptus globulus (eucalyptus) are used for the treatment of diabetes mellitus in traditional medicine. The aim of this study was to evaluate the effects of eucalyptus on streptozotocin (STZ)-induced damage in pancreatic islands by stereological methods. Fifty mature normoglycaemic male Wistar rats, weighing 200–250 g, were selected and randomly divided into 5 groups (n = 10): control; STZ-induced diabetic (D) — by intraperitoneal injection of 60 mg/kg streptozotocin; treated control (TC); and treated diabetic (TD₁, ₂), respectively, received 20 and 62.5 g/kg of eucalyptus in their diet, and 2.5 g/L aqueous extract of eucalyptus in their drinking water from one week after induction of diabetes. After four weeks of the experiment, stereological estimation of volume density and total volume of islets and beta cells, volume-weighted mean islet volume, mass of the islets and pancreas, and total number of islets were carried out. Administration of eucalyptus significantly decreased the weight loss and increase of water and food intake in the treated diabetic groups in comparison to the STZ-induced diabetic (D) group. Volume density and total volume of islets, volume-weighted mean islet volume, mass of islets, and mass of pancreas of both treated diabetic groups were higher than the D group. In TD₂, these stereological parameters increased significantly compared to the D group (p < 0.001). Volume density and total volume of beta cells increased 21% and 65%, respectively, in the TD₂ group, but it was not statistically significant compared to the diabetic group (p > 0.05). The results suggested that Eucalyptus globulus with a dose-dependent manner ameliorates diabetic states by partial restoration of pancreatic beta cells and repair of STZ-induced damage in rats. This study suggests a beneficial effect of eucalyptus in the treatment of diabetes. (Folia Morphol 2010; 69, 2: 112–118)
The present survey was undertaken to explore the traditional knowledge of plant species found in four villages (Athamangalam, Vadukkuveli, Thalainayar and Perumangalam) in Sirkali taluk of Nagapattinam district, Tamilnadu state, The ethnomedicinal information was collected through oral interviews to village elders and vaidyas (those who cure disease by traditional methods) of the rural areas of this district. The knowledge available to the rural population of the area is transmitted only through oral language from generation to generation over time and therefore it needs to be recorded in order to be preserved. Medicinal plants are listed by botanical name, family name, local name, plant parts used and mode of preparation and the names of the diseases cured. About 50 plant species belonging to 27 families were described as effective herbal drugs for various ailments. The documented ethnobotanical uses of plants mostly related to the treatment in curing stomach pain, breast pain, headache, bleeding, yellow fever, muscle pain and blood purifier.
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This paper contains significant ethnobotanical information on folk-medicinal plants and their ethnopharmacological uses in Kadięehri. The aim of the study was mainly to collect and identify the plants used therapeutically by the local people, and to make available information about traditional herbal medicine. It was undertaken during the period 2011-2012 and is based on plants collected during field work. Fifty-six plants used in folk-medicine and belonging to 34 families were identified in this study. Of these, 48 species were wild, and 8 species were cultivated plants. The most common families were Rosaceae (12.5%), Lamiaceae (8.9%) and Asteraceae (7.1%); and the most common preparations were decoctions (36.7%). In addition, a cultural importance index (CI) and use report (UR) were calculated for each species. Based on the CI, the most important plants were Cydonia oblonga (0.77), Ecballium elaterium (0.66), Urtica urens (0.66), Vitis vinifera (0.66), Plantago lanceolata (0.65), Plantago major subsp. major (0.65) and Rosa canina (0.62). We found three species of plant (Astragalus noaeanus, Populus xcanescens and Salvia cyanescens) which had never before been reported to have medicinal properties.
The aim of this report is giving the importance in need of ground leveal documentation of medicinal plants, their ingrediants and usage. To be effort has been made to evaluate plants used for medicare by the tribal people of Renla Gadda Thanda, Kodangal mandal, Mahabubnagar Dist, Telangana. Due to be deficient in of modern healthcare facilities and poor economic conditions, the tribal people of the Thanda fully depend on in the vicinity available medicinal plants for their healthcare needs. In the present report a total of 24 ethnomedicinal plants from 23 genus and 24 species occupied 18 families were recorded. The thorough information i.e., Usage, to be treated disorder, Parts used recorded. This is a first report from the Renlagadda Thanda of Kodangal mandal. The study area having wealthy in medicinal plants flora and tribal communities with latest usages. Documentation of traditional knowledge on medicinal plants from the village or thanda level is the elementary and necessary samples for novel medication.
Jasminum auriculatum belonging to the family Oleaceae are reported to have good medicinal values in traditional system of medicines. The present study deals with pharmacognostical examination of morphological and microscopical characters and phytochemical investigations of Jasminum auriculatum leaves including determination of loss on drying, ash values and extractive values. The preliminary phytochemical screening of powdered drug was also carried out, the qualitative chemical examinations revealed the presence of various phytoconstituents like alkaloids, steroids, terpenoids, saponins, carbohydrates, Glycosides, protein, mucilages, phenols ,tannins and flavonoids.
The present investigation has been carried out to find the ethno medicinal value of plants in Thanjuvur District of Tamil Nadu, India. This study of ethno medicinal value of plants and cure the various disease of around the Thanjuvur District. Ancient methods of treatment by ethno medicinal value of various types of trees, shrubs, herbs and underground modification such as root, stem, bark, leaves, flowers, fruits and seed. The ethno medicinal value of plants make grounded plant parts, and juices used to cure the various diseases like, cough, cold, asthma, eye disease, ulcer, jaundice, rheumatic arthritis, memory stimulants, wound healing and cardiovascular disease. We have identified and reported more than 38 species of medicinally important plants belonging to 25 families.
Due to toxic and adverse side effects of synthetic drugs, traditional herbal medicine has the potential as a source of new bioactive molecules. That is why we investigated this research, searching new anti-inflammatory drugs from plants growing around us. The anti-inflammatory effect of the mixture of different plants used in traditional medicine (Alkanna tinctoria, Rubia tinctorum and Artemisia herba alba) were studied using carrageenan-induced paw edema (oil mixture extract 100 mg/kg and 200 mg/kg). These material reduces carrageenan-induced inflammation in rats and shows inhibition after 4 h especially at a concentration 200 mg/
We studied medicinal and food plant species, recording an extraordinary number of species and uses in Castilla-La Mancha, Murcia and Valencia in Spain. Focusing on two demographically distinct regions - Castilla-La Mancha and Lower Segura Valley. A high proportion of the flora (20 to 30 %) is known for its medicinal properties, and, interestingly, a high number of medicinal-food plants (5 to 7 %) is recorded. The concept of "Local Food" involves the whole repertory of species that characterises the local diet (incl. local cultivars and non-cultivated gathered food plants). The number of food plant species varies between 15 and 25 % of the vascular flora, and for the gathered food plants (GFP) this decreases to a 3 to 8 %. Those GFP that are also used for medicinal purposes are only 2 to 4 % of the total vascular flora. The relevant plant families are very similar in relative numbers along the different areas: Compositae, Rosaceae and Umbelliferae, followed of Boraginaceae, Liliaceae, Cruciferae, and Caryophyllaceae. Chenopodiaceae, Polygonaceae and Gramineae are less unifomly represented or in lesser numbers. The high species diversity does not imply a general dietary relevance of this particular plant family. For instance Caryophyllaceae with a relatively low number of species comprises the "collejas" (Silene vulgaris) that have shown to be the more widely consumed species. Information regarding 145 species has been recorded. Among the Gathered Food Plant Species 81 are used in medicine, in double proportion than the cultivated food plants. 61 are orally administered, in the same form as food.
Ayurvedic medicines are considered to be the best systems of treatment in India and this system is spreading now globally. Natural products are also a part of our everyday life. Ethno veterinary medicine is developed by farmers in field and barns rather than and in scientific laboratories. It is less systematic, less formalized and usually transferred by word of mouth rather than writing. An ethnobotanical survey was conducted in 10 selected sites of Villupuram district. Twenty six plant species belonging to fourteen families were documented in the present study, to cure different diseases in animals. Interviews and detailed personal discussions were conducted with the traditional healers and local people to identify the plants and their medicinal information for six months. The study revealed that the different parts of these plants were used for treatment of different diseases. Leaves are the mostly used part to prepare medicine.
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Phenolics in the Tussilago farfara leaves

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Coltsfoot leaves (Farfarae folium) are used in the European medicine in respiratory tract diseases, for cough, bronchitis and asthmatic disorders, while in the traditional Chinese medicine only flower buds (Farfarae flos) have been recognized as a medicine. A short literature review shows that most data concern the chemical composition of the coltsfoot flowers. During the carried out studies we have isolated and identified (UV, 1H and 13C NMR, analysis of acid and enzymatic hydrolyze products) six known flavonols from the coltsfoot leaves: kaempferol and its 3-O-β-glucopyranoside and 3-O-α-rhamnopyranosyl(1→6)- β-glucopyranoside, along with quercetin derivatives: 3-O-β-arabinopyranoside, 3-O-β- glucopyranoside and 3-O-α-rhamnopyranosyl(1→6)-β-glucopyranoside. Moreover, we have detected the presence of three phenolic acids.
Out of 750,000 known plants in the world, a major part are medicinal and aromatic plants – a source of raw material for folk and documented systems of medicines worldwide. The folk and documented medicine in India use about 6,000 plants, although, less than 50 species have been scientifically studied and cultivated to any sizeable extent. The main factor behind the slow pace of domestication of medicinal plants is the absence of knowledge on cultivation practices and lack of suitable technology. About 90% of the medicinal plants for trade are harvested from the wild and the demand for traditional medicinal plants is increasing rapidly. Continuous exploitation of several medicinal plant species from the wild has resulted in their population decline. Hence, an effective strategy is needed for their sustainable utilization and conservation. Cultivation is the most effective way of conservation. Cultivation can also ensure production of standardized raw materials. Thereby, enhances the quality of the manufactured products. The methods and techniques of modern chemical agriculture cannot be adopted for the cultivation of medicinal plants as they should be free from harmful residues. Pesticides and other harmful chemicals have been detected in some herbal products. Hence, to ensure a safe, residue-free and reliable material for use in herbal drug industry, there is an urgent need to adopt strategies for cultivation of medicinal plants that are consistent with principles of good agricultural practices.
Background. Saharan plants are known by their high content of antioxidant products like phenolic compounds due to the extreme climatic conditions. They constitute the basis of treatments used by local population for various diseases. The purposes of this study were to measure the total phenolic compounds and total flavonoid compounds, to determine antioxidant capacity, and to evaluate the antibacterial activity of three wild Saharan medicinal plants. Material and methods. Hexane and ethyl acetate fractions of ethanokwater extract and the residu of the extracted aqueous layer of Ferula vesceritensis fruits, Genista saharae aerial parts and Zilla macropterae fruits were assayed to determine their antibacterial activity using the disc diffusion method against: Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853). In addition, the total phenolic compounds and total flavonoids and antioxydant activity using DPPH test of ethyl acetate fractions (EAF) of plant parts studied were investigated. Gallic acid, quercetin and vitamin C were used for these parameters. Results. Among the extracts tested, ethyl acetate fractions of all plants and hexane fraction of F. vesceritensis showed activity against S. aureus. Good activity was shown by EAF of G. saharae. According to the results, it is observed that Z. macropterae fruits possess a good antioxidant activity. Conclusion. The results indicate that the ethyl acetate fraction of G. sahara Aerial parts possesses a good antibacterial activity against S. aureus, which justifies its use in traditional medicine for treating respiratory diseases. Furthermore, evaluation of in vitro antioxidant capacity of Ethyl acetate fractions of these plants, particular Z macroptera fruits, has also provided interesting results. Zilla macroptera fruits may therefore be a good source of antioxidants.
The folk herbal medicine among the ethnic communities Bakhtyari and Chaharmahali in Chaharmahal and Bakhtyari districts, South-West of Iran, was studied. A total number of 61 medicinal plant species belonging to 23 families are described. The most commonly represented families were Lamiaceae (18%), Asteraceae (13%), Fabaceae (11.5%) and Apiaceae (11.5%). Most of the uses were found to be new when compared with published literature on ethnomedicine of Iran. In general, inhabitants of the studied area still have a strong belief in the efficiency of medicinal plants. The results of the study reveal that some of species play an important role in primary healthcare system of these tribal communities.
Introduction: In Iranian traditional medicine, Zataria multiflora Boiss (Lamiaceae family) is reputed due to its antiseptic effects. Objective: The purpose of this study was to evaluate the antibacterial and biofilm killing effects of Z. multiflora essential oil and main components against Pseudomonas aeruginosa. Methods: The main components of essential oil were identified by gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS). The antibacterial properties of Z. multiflora oil and main components were determined by assessing the MIC and MBC values, and their inhibition percent of biofilm killing effects were determined by the evaluation of optical density. The role of each main component in these activities was determined according to the chemical profiles of essential oil. Results: Thymol (38.7%), carvacrol (30.6%), and p-cymene (8.3%) were main components of twenty five components of essential oil. Carvacrol had the higher role in antibacterial activity against P. aeruginosa, followed by thymol. P-cymene enhanced the antibacterial activities of thymol and carvacrol against P. aeruginosa. Carvacrol showed the weak role in biofilm killing effect. In spite of the low antibacterial activity of p-cymene against P. aeruginosa, it can enhance the antibacterial activity of thymol or carvacrol. Conclusion: Z. multiflora essential oil can be used for the management of P. aeruginosa infections. Determining the precise role of each components needs investigating in their behavior in different media.
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