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Parameters of variability and diversity of tetrameric STRs for practical use in Zlotnicka White and Zlotnicka Spotted pigs

100%

Vrtkova I., Filistowicz A., Buczynski J. T., Vrtek S., Falkova L.

Animal Science Papers and Reports

2017

tom 35

nr 3

As a goal of this paper, the assessment of genetic variability of Złotnicka White (ZW) and Zlotnicka Spotted (ZS), was chosen in order to verify the appropriateness of 11 tetrameric Short Tandem Repeats (STRs) panel for use in genetic resources of pigs. Analyses were carried out in sets of 91 ZW and 250 ZS pigs. Seventy-one alleles in ZW and 85 alleles in ZS were detected at all 11 STRs loci. An average number of alleles at locus (MNA) was 6.455 in ZW and 7.727 in ZS. An average number of effective alleles (MNe) was 3.532 in ZW and 3.431 in ZS. Observed heterozygosity Ho was 0.659 in ZW and 0.637 in ZS. On average, polymorphism information content (PIC) reached 0.639 and 0.619 per locus in ZW and ZS. The probability of identity of two independent samples PI using all 11 STRs loci in ZW amounted to 3.118 x 10⁻¹⁰ and to 5.921 x 10⁻¹⁰ in ZS while the probability of identity related individuals PISibs was 1.331 x 10⁻⁴ and 1.749 x 10⁻⁴ in ZW and ZS. The power of exclusion for loci combinations when both parents are known, when only one of the parent is known and for two putative parents P1, P2 and P3 were in ZW versus ZS 0.99903 v. 0.99887, 0.97998 v. 0.97654 and 0.99999 v. 0.99998, respectively. Identified estimates of stated parameters illustrate suitability of tetrameric STRs for practical application in the management of genetic resources, verification of parentage and traceability in ZW and ZS. Based on the results, we recommend the panel of tetrameric STRs loci as suitable for parentage, traceability and differentiation of subpopulations in genetic pig resources of similar history. Hₑ.

Crystal structure of rat transthyretin at 2.5 A resolution: First report on a unique tetrameric structure

100%

Wojtczak A.

Acta Biochimica Polonica

1997

tom 44

nr 3

The first observation of a unique tetrameric molecular structure of transthyretin from rat (rlTR, prealbumin) is reported. The structure has been determined by X-ray diffraction using molecular replacement and the structure of human transthyretin (hTTR) as a starting model. Crystals of native rat transthyretin are tetragonal, space group P4 3 2 1 2, and have four independent monomers in the asymmetric unit of the crystal lattice. Data were collected to 2.5 A resolution and the structure has been refined to R = 18.9% for 13584 data points between 6-2.5 A resolution. Like hTTR, the rat protein is also a 54000 Da tetramer with four identical polypeptide chains of 127 amino-acid residues. Of the 22 amino-acid residues which are different in the human and rat TTR sequences, none are in the thyroxine binding domain. Analysis of these data reveal that the tertiary structure of rTTR is similar to that of hTTR with only small differences in the flexible loop regions on the surface of the protein. As a result of local changes in flexible loop regions near residues 30-41, 60-65 and 102-104, the structure of rTTR monomers is more compact than that of the corresponding hTTR monomers. The loop between residues 30-41 is bound closer to the monomer core in the former as compared with the latter structure and there is a wider opening of the space formed between these loops at two adjacent monomeric subunits. These conformational changes do not affect the interfaces between the monomeric subunits and are not transmitted to the thyroxine binding site so that its topology remains not altered.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Animal Science Papers and Reports

1 Buczynski J. T.

1 Falkova L.

1 Filistowicz A.

1 Vrtek S.

1 Vrtkova I.

1 Wojtczak A.

1 2017

1 1997

Wyniki wyszukiwania

Parameters of variability and diversity of tetrameric STRs for practical use in Zlotnicka White and Zlotnicka Spotted pigs

Crystal structure of rat transthyretin at 2.5 A resolution: First report on a unique tetrameric structure