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Systemy oceny kompetencji rozwojowej oocytów oraz zarodków ssaków oparte na technologii mikrofluidycznej typu Lab-on-Chip

100%

Kempisty B., Walczak R., Antosik P., Szczepanska P., Wozna M., Bukowska D., Zaorska K., Dziuban J., Jaskowski J.

Medycyna Weterynaryjna

2011

tom 67

nr 01

This review presents basic criteria for evaluating the developmental competence of oocytes and embryos, and contains a detailed description of the microfluidic-technology-based Lab-on-Chip. The developmental competence of oocytes is acquired through a complex process associated with oocyte growth and maturation, the storage of large amounts of mRNA and proteins, and with the formation of proper cell morphology. The full maturation of oocytes is required for successful monospermic fertilization and embryonic preimplantation development. The morphology of the gamete is one of the most important factors influencing the developmental competence of the cell. There are several indicators for the assessment of oocyte morphology, most of them including the color and granularity of the cytoplasm. Intensive research is under way to develop and introduce new non-invasive methods of oocyte and embryo quality assessment as a major factor in the improvement of assisted reproductive techniques. The Lab-on-Chip technology, as an independent micro-cytometric device, is a combination of reproductive biology techniques and micro-optic electronics. In the future, Lab-on-Chip systems may be used as an important diagnostic instrument for evaluating the quality of mammalian oocytes and embryos.

Zastosowanie mikrochipu fluidycznego typu Lab-on-Chip do oceny jakości oocytów i zarodków ssaków

100%

Kempisty B., Walczak R., Sniadek P., Piotrowska H., Wozna M., Jackowska M., Bukowska D., Antosik P., Dziuban J., Jaskowski J. M.

Medycyna Weterynaryjna

2011

tom 67

nr 08

The developmental competence of oocytes and embryos is one of the basic factors in determining whether the oocytes will be able to grow and mature and whether the embryos will be able to develop in the preimplantation stage and implant properly. There are several methods of evaluating the developmental competence of oocytes and embryos. The most frequently used include the oocyte maturity test using brilliant cresyl blue (BCB), and molecular analyses, such as genomics, transcriptomics, and proteomics. The Lab-on-Chip (LOC) system is a microcytometer that analyzes the spectrum of light absorption. The analysis of oocyte and embryo competence with this system concerns the thickness and color of the zona pellucida, as well as the color and granularity of the cytoplasm. The microfluidic chip includes two micro fibers and two channels: one leading in and one leading out. The total measuring system comprises a docking station with a chip, micro-spectrophotometer, and laptop. The system can therefore be used under non-laboratory conditions. Analysis with this system is a new and noninvasive method of bovine and porcine oocyte and embryo quality assessment, and may in the future be one of the main tools used in evaluating the developmental competence of these cells. This article presents the possible function and use of a system based on the microfluidic technology. Moreover, the construction and applications of the LOC are discussed, including the possibilities of LOC application in analyses of the developmental competence of bovine and porcine oocytes and embryos.

Ocena kompetencji rozwojowej oocytów oraz zarodków świń na podstawie badań molekularnych i mikrofluidycznych

80%

Kempisty B., Walczak R., Sniadek P., Dziuban J., Piotrowska H., Zawierucha P., Zaorska K., Antosik P., Bukowska D., Jaskowski J. M., Nowicki M., Zabel M.

Medycyna Weterynaryjna

2011

tom 67

nr 10

Evaluation of oocyte developmental competence has an important influence on the ability of these cells to attain maturation, successful fertilization, development of embryo to the blastocyst stage and proper implantation. Factors determining the reproductive potential of gametes included: (1) expression of important transcription factors, (2) epigenetic changes, which influence the silencing of selected genes transcription, (3) transcription regulation, and (4) post-transcriptional regulation. The epigenetic changes mainly include: DNA methylation, histones modifications and changes in chromatin structure in the oocytes. In several studies, the association between oocyte morphology (mostly determined by cumulus cell layers and granularity of cytoplasm) and the ability of these cells to attain maturation and fertilization has been described. The use of biochemical, metabolomical and molecular markers is the most frequently applied tool in the assessment of oocyte developmental competence. However, most of these methods are invasive and lead to the decreased viability of the analyzed cells. Searching for new, objective and noninvasive techniques leads to the development of a microfluidic chip system, which shows the physical (spectral) properties of oocytes and embryos in comparison to the biological parameters. In this article, selected issues associated with the genetic regulation of such processes as: maturation of oocytes, fertilization and early stages of embryonic development, have been presented. Moreover, the regulation of transcription and post-transcriptional modification during oogenesis and embryogenesis in mammals, with special relation to pigs and the possibilities of applying of microfluidics in assessment of oocyte and embryo developmental competence was shown.

Ograniczanie wyników

3 Medycyna Weterynaryjna

3 Antosik P.

3 Bukowska D.

3 Dziuban J.

3 Kempisty B.

3 Walczak R.

2 Jaskowski J. M.

2 Piotrowska H.

2 Sniadek P.

2 Wozna M.

2 Zaorska K.

1 Jackowska M.

1 Jaskowski J.

1 Nowicki M.

1 Szczepanska P.

1 Zabel M.

1 Zawierucha P.

3 2011

Wyniki wyszukiwania

Systemy oceny kompetencji rozwojowej oocytów oraz zarodków ssaków oparte na technologii mikrofluidycznej typu Lab-on-Chip

Zastosowanie mikrochipu fluidycznego typu Lab-on-Chip do oceny jakości oocytów i zarodków ssaków

Ocena kompetencji rozwojowej oocytów oraz zarodków świń na podstawie badań molekularnych i mikrofluidycznych