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1

Development of somatic embryos from suspension culture of Salix viminalis L.

100%
Wojciechowicz M. K., Zenkteler E.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2008
|
tom 50
|
nr 1
2

Effect of calcium restriction on cardenolide accumulation in two cell lines of Digitalis thapsi grown under differend light regimes

100%
Cacho M., Moran M., Corchete P., Fernandez-Tarrago J.
Acta Physiologiae Plantarum
|
1999
|
tom 21
|
nr 4
3

Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures

100%
Mikula A., Fiuk A., Rybczynski J. J.
Acta Biologica Cracoviensia. Series Botanica
|
2005
|
tom 47
|
nr 1
The paper describes structural and ultrastructural changes in primary explants, induction of embryogenesis, somatic embryo development, and four protocols for cryopreservation of cell suspensions. The changes during tissue culture of hypocotyl and cotyledon explants from 10-day-old seedlings and fragments of leaf explant of Gentiana cruciata L. were studied. Seedling explants were cultured on MS medium supplemented with 1.0 mg/l dicamba + 0.1 mg/l NAA + 2.00 mg/l BAP + 80.0 mg/l adenine sulphate. The hypocotyl callus tissue was initiated by cell divisions of the vascular cylinder, but in cotyledons only parenchyma cells took part in callus formation. The leaf blade expiants usually responded only by proliferation of the wounded surface. The effect of auxins (2,4-D, NAA, DIC) and cytokinins (kinetin, zeatin, BAP) in various concentration and combinations on leaf explant response was examined. Generally, embryos were formed sporadically on media containing NAA (1.64% responding explants) or 2,4-D (0.38%), but were not produced in the presence of dicamba. Production of somatic embryos was more effective from suspension culture than from agar medium. Liquid culture made it possible to maintain the cell suspension’s embryogenic competence for 5 years. For preservation of proembryogenic masses, four protocols of cryopreservation were studied: direct cooling, sorbitol/DMSO treatment, vitrification, and encapsulation. Direct cooling and sorbitol/DMSO treatment was unsuccessful. Vitrified tissue required a minimum 3 weeks of culture on solid medium for cell proliferation to reach the proper fresh weight for manipulation. Alginate beads with PEMs were transferred directly to liquid medium for post-freezing culture. Vitrification and encapsulation maintained high viability of post-freezing PEM, but encapsulation ensured faster restoration of G. cruciata cell suspension.
4
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Ephedrine production from suspension cultures of Ephedra alata L. callus

84%
Hegazi G. A., Ghareb H. E., Gabr M. F.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2020
|
tom 101
|
nr 1
5

The potential to produce cynarin in Cynara scolymus suspension cultures in relation to their antioxidant profile

84%
Parada D., Wielanek M., Kuzniak E., Sklodowska M.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
6

Effect of Polyscias filicifolia Bailey extracts on the behavior of the leaf miner Cameraria ohridella Deschka and Dimic on horse chestnut trees

84%
Tomczyk A., Kropczynska D., Ptak A., Titova M., Shumilo N., Oreshnikov A., Nosov A., Furmanova M.
Acta Biologica Cracoviensia. Series Botanica
|
2007
|
tom 49
|
nr 1
55-60
Extract of Polyscias filicifolia suspension culture was used to reduce damage to horse chestnut (Aesculus hippocastanum) leaves by larvae of the horse chestnut leaf miner (Cameraria ohridella). The results showed the repellent effect of biomass extracts on Cameraria ohridella moths. The number of moths caught per day on sticky traps treated with extract was more than 50% lower than the control. The same extract on leaflets caused C. ohridella females to lay ~20% fewer eggs than on leaflets without extract. P. filicifolia extract had a repellent effect on female moths in laboratory conditions as well. Only single mines were observed on leaves treated with P. filicifolia extract, five times less than on control leaves. The data indicate that P. filicifolia extract can be used as a repellent for C. ohridella in springtime when the overwintering generation emerges from pupae.
7

Glycanase activities in lupin roots, callus and suspension cultures of different resistance, infected by Fusarium culmorum

84%
Kuzniak-Gebarowska E., Urbanek H., Sakowski R.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1990
|
tom 38
|
nr 1-12
8

The potential of producing cynarin in Cynara scolymus L. suspension cultures in relation to their antioxidant profile

84%
Parada D., Wielanek M., Kuzniak E., Sklodowska M.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 524
Recent studies revealed that reactive oxygen species, antioxidants and related redox signals are key elements in the regulation of plant defense responses, including the synthesis of secondary metabolites. In this work the potential of artichoke (Cynara scolymus L.) cell suspensions to produce cynarin was studied in relation to the antioxidant profile of the cultures. There were determined the total antioxidant capacity and concentrations of H₂O₂, ascorbate and total phenolics, as well as activities of ascorbate and guaiacol peroxidases, ascorbate oxidase and catalase at different stages of the culture growth cycle in relation to the production of cynarin. Suspension cultures revealed a growth-linked capacity to produce cynarin as identified by qualitative HPLC analysis. The cynarin accumulation kinetics during the exponential phase of growth correlated with the total antioxidant capacity of water-soluble antioxidants whereas the profiles of total phenols and total antioxidant capacity of water-insoluble antioxidants differed. The lowest ascorbate-related antioxidant capacity coincided with the maximum cynarin accumulation in the early stationary phase. The regulation of growth-related prooxidant/antioxidant balance in the artichoke suspension cultures involved the peroxidase/phenolics/ascorbate system scavenging H₂O₂, further affected by ascorbate- and H₂O₂-metabolizing enzymes. These results may be of interest in biotechnological strategies aimed at optimizing the production of secondary metabolites in plant cultures in vitro.
9

Secretion of stress-related proteins by suspension-cultured Lupinus albus cells

84%
Wojtaszek P., Pislewska M., Bolwell G. P., Stobiecki M.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 1
A suspension culture of white lupin cells has been established, and proteins of the exocellular matrix analysed. Based on homologies of N-terminal amino-acid sequences, three stress- or defence-related proteins: acidic class III chitinase, polygalacturonase-inhibiting protein, and germinóxalate oxidase, secreted by lupin cell culture, were identified.
10

Immunolocalization of calreticulin in the protoplasts and somatic embryos of Daucus carota L. grown in suspension culture

84%
Libik M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
1999
|
tom 41
|
nr 1
11

In vitro morphogenesis of callus cells of Capsicum annuum [L.] from suspension cultures

84%
Gatz A., Lewicka E.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
12

Callus tissues of Rhodiola kirilowii [Regel] Maxim. - dynamic of growth and active compounds production

67%
Krajewska-Patan A., Dreger M., Buchwald W., Gorska-Paukszta M., Mielcarek S., Baraniak M., Mscisz A., Furmanowa M., Mrozikiewicz P. M.
Herba Polonica
|
2009
|
tom 55
|
nr 3
222-230
Rhodiola Kirilowii (Regel) Maxim. (Crassulaceae) is a traditional medicinal plant used in North Asia and China, especially in the cardiopulmonary disorders in the hypoxic conditions induced by high altitude. The presented results are the part of the investigations carried out in the Branch of Medicinal Plants of the Institute of Natural Fibres and Medicinal Plants in cooperation with the Department of Biology and Pharmaceutical Botany, Medical University in Warsaw on R. Kirilowii plants and tissue cultures. The aim of recent study was to determine the growth dynamics and active compounds production during the cultivation of callus tissues of R. Kirilowii on solid/liquid media. Tissue cultures of R. Kirilowii shown the ability to produce all the active compounds determined in the roots of plants of Polish origin. It is worth emphasizing, that rosavins, according to known literature, were not detected in roots of plants growing in Asia. The best time for collection the tissues from solid medium was fifth or sixth week of the culture – the tissues were growing dynamically and the contents of the main active compounds was high. The material from suspension should be collected in 12–15 days after inoculation. The obtained results will be applied in future investigations on the use of R. Kirilowii extracts in the experimental hypoxia in rats.
13

Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells

67%
Sobkowiak R., Rymer K., Rucinska R., Deckert J.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 1
Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd2+ either stimulates (1–4 uM) or inhibits ( 6 uM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767–72). Here, soybean cell suspension cultures were treated with various concentrations of Cd2+ (1–10 uM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd2+ concentration in culture medium increases only slightly, from 4 to 6 uM.
14

Cucumber embryogenetic haploid suspension cultures

67%
Klos M., Kondo K., Niemirowicz-Szczytt K., Burza W.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
15
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Content available

Biochemical characterizations of anther-derived and inhibitor-selected mutants with enhanced lysine in rice [Oryza sativa L.]

67%
Schaeffer G. W., Sharpe F. T., Dadley J. T.
Journal of Applied Genetics
|
1998
|
tom 39
|
nr 1
The nutritional quality of cereals, including rice, would be improved by increasing the lysine content. Experiments were designed to test whether cell lines and plants could be recovered from in vitro selections. Mutants of rice recovered from inhibitory levels of lysine plus threonine and S(2-aminoethyl)cysteine from anther-derived calli were regenerated and characterized at the whole plant, cell, and enzyme level. These procedures produced plants with 15% higher lysine in milled grains than the starting cultivar and provide excellent materials for basic studies in cell selections, lysine biochemistry, stress-related biochemistry and gene isolations. A tissue culture line is now available that exports protein more efficiently than the wild type cells and provides materials for the study of mechanisms of expression of the recessive gene(s) conditioning the enhanced lysine phenotype.
16

Cell enlargement in suspension culture of carrot [Daucus carota L.Cv.Karo]. Preliminary studies

67%
Golczyk H.
Acta Biologica Cracoviensia. Series Botanica
|
1998
|
tom 40
Cytological studies of Daucus carota L. (cv. Karo) in suspension culture were made. Cells growing on medium without ammonium were larger and more vacuolated than were cells grown on the standard medium. Their sizes (cell area) ranged widely from 200 µrm2 to 40000 µm2. They also showed a very low level of aggregation (1%) as compared to an 81% frequency of aggregates in the standard suspension. Suspension deficient in ammonium had a .small percentage of 2-9-nucleated cells. Such multlnucleate cells were not observed in suspension with both nitrate and ammonium used as nitrogen source.
17

Anthocyanins in suspension cultures originating from different parts of sweet potato plant

67%
Konczak-Islam I., Yoshinaga M., Nakatani M., Yamakawa O.
Biotechnologia
|
1999
|
nr 3
18

Wpływ elicytacji jasmonianem metylu na akumulację kwasów fenolowych w kulturach zawiesinowych Ginkgo biloba L.

59%
Szewczyk A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 524
Określono wpływ jasmonionianu metylu na akumulację fenolokwasów w kulturach zawiesinowych Ginkgo biloba. Kultury okazów żeńskich prowadzono na podłożu Murashige - Skoog’a z dodatkiem pikloramu (4 mg·dm⁻³) oraz BA (2 mg·dm⁻³). Jasmonian metylu w stężeniu 25 µM dodawano w 14 dniu od założenia hodowli. Analizy ekstraktów metanolowych z biomasy prowadzono metodą HPLC. Detekcję aglikonów fenolokwasów prowadzono po hydrolizie ich glikozydów 2 mg·dm⁻³ wodnym roztworem HCl. Wyniki wskazują, że dodatek jasmonianu metylu może stymulować kultury zawiesinowe Ginkgo biloba do zwiększenia produkcji kwasów fenolowych, szczególnie ferulowego i syryngowego.
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