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The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved. In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.
With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.
In this study buckwheat flour was used as a supplement of a commercial gluten-free formulation (MLECZNA and NISKOBIAŁKOWA). The effect of 30 or 50% substitution of formulation basis with buckwheat flour on the technological and rheological properties of dough was observed. The dough was analysed for contents of proteins, elements and resistant starch. Viscoelastic properties of the examined dough were measured using Rheostress 1 (ThermoHaake) in an oscillation mode. Changes in the viscoelastic properties were expressed as elastic modulus (G’) and viscous modulus (G”). The substitution of 30 or 50% of the gluten-free formulation basis with buckwheat flour increased the level of total proteins, macro- and microelements, as total mineral components, and resistant starch. Irrespective of the level of buckwheat flour in gluten-free formulations a decreasing water binding capacity was confirmed. Dynamic rheological studies of gluten-free dough of MLECZNA formulation showed the higher elastic (G’) and viscous (G”) moduli in comparison with NISKOBIAŁKOWA formulation. Both analysed moduli decreased when buckwheat flour replaced a part of the analysed commercial gluten-free formulations. It indicated that the buckwheat flour could diminish the structural function of compounds substituting for gluten in gluten-free formulations. Based on the results obtained it could be concluded that buckwheat flour is a promising valuable supplement of gluten-free formulations.
The study was aimed to estimate changes in the use of dietary supplementation with preparations containing magnesium among adult inhabitants of Warsaw. The survey was carried out in 1994 on 396 and in 1999 on 336 persons. Respondents were randomly selected. A significant increase of the percentage of people using preparations containing magnesium was observed (from 28% to 41%). Analysis of the influence of demographic factors on the frequency of magnesium supplementation showed changes in the demographic structure of respondents using preparations with magnesium. In 1999 women used supplements more often than men; the frequency of use increased also with age and educational level. The supplements were used significantly more often by people suffering from chronic diseases. No differences between magnesium doses used in 1994 and 1999 were observed. The median dose was 67 mg per day in 1994 and 100mg per day in 1999. The percentage of persons using magnesium in doses exceeding the upper limit of intake (350 mg per day) declined significantly (from 7% to 0,7%).
Food supplementation studies often assume that animals will select artificial diets in their natural environment, and that high-protein, high-energy foods are the most appropriate supplement. These assumptions were tested in red-backed voles Clethrionomys gapperi Vigors, 1830 using food-choice experiments with sunflower seeds, oats, and commercial diets in the laboratory and field. Preferred level of dietary protein was also examined using isocaloric diets that varied only in protein content (14%, 20% and 30%). Preferences exhibited in the above trials were subsequently examined relative to natural forage. Voles demonstrated a strong preference for sunflower seeds over oats, dried alfalfa, and rabbit, guinea pig, rat and cat food. Voles preferred the 14% protein diet over the 20% and 30% protein diets. Although sunflower seeds contain more than 20% protein, voles consistently preferred this food over natural forage, perhaps because of their high fat and energy content. This indicates that tradeoffs in protein content may be made to maximize energy. We suggest that red-backed voles will select sunflower seeds in their natural environment, and that their preference for low protein likely reflects their herbivorous diet. This study highlights the importance of an a priori understanding of species-specific preferences and requirements when designing food supplementation studies.
The aim of the study was to describe the role of psychosocial determinants of using vitamin and mineral supplements among students. Through using the theory of planned behaviour, a more detailed approach to assess the psychosocial factors influencing dietary supplement use among students was created. Attitude toward behaviour was the statistically significant factor influencing both intention and behaviour concerning use of vitamin and mineral supplements among the population. Subjective norm and perceived behavioural control was not a good predictor of using supplements among the population.
Background. Athletic performance can be substantially enhanced with supplements and functional food which are considered by scientists as efficient, safe and legal, such as protein, carbohydrate and protein-carbohydrate supplements, isotonic sports drinks, carbohydrate-protein bars, carbohydrate bars, creatine and caffeine. Objective. The study is aimed at an analysis and evaluation of the prevalence of using effective ergogenic aids (creatine, caffeine, isotonic drinks, carbohydrates, and proteins) in a group of Polish professional athletes. Material and Methods. The research was conducted on 600 athletes (216 women, 384 men) practicing various sports disciplines; the younger group (18-23 years old) consisted of 307 people, while the older one (24-35 years old) was comprised of 293 subjects. A questionnaire was used with questions concerning the frequency and types of consumed supplements. Results. Nearly half of the athletes (48,2%) admitted to taking supplementation, of which 36.7% consumed the supplements occasionally and 11.5% continually. The majority of the group (75.4%) claimed to be consuming isotonic drinks, which were the most commonly chosen nutritional aid enhancing physical performance, most frequently supplementing the diet in a continuous manner (41.2%). The least frequently used supplement was creatine, chosen by only one in three interviewees (34,5%). The ergogenic aids were used more often by men than women (50.5% vs. 44.1%), and so were nutrients based on proteins (51.8% vs. 32.0%), carbohydrates (60.7% vs. 46.8%), protein-carbohydrates (45.6% vs. 32.9%), as well as creatine (39.8% vs. 25.0%). The studies showed the inessential difference in the frequency of taking supplementation based on the interviewees’ age (0.4%). Conclusions. Competitors who use supplements over those who choose not to, seems to reflect the continuous lack of the athletes’ sufficient awareness of the effectiveness, safety, and health benefits of dietary supplementation that enhances physical performance.
The paper has aimed to determine total polyphenol compound content and antioxidant ac­tivity of fresh aloe leaves and aloe preparations obtained experimentally and manufactured. Research material constituted fresh sieved aloe leaves, aloe gel obtained experimentally from the leaf inner, pulp made of sieved aloe leaf skin and manufactured aloe gel - a diet supplement. Most of polyphenol compounds were found in skin of aloe (390.8 mg/100 g) which showed the highest antioxidant activity (6.6 jumol ofTrolox in 1 g of skin). The next position was deserved to whole leaves (213.2 mg of GAE in 100 g of preparation - 4.2 ¿umol ofTrolox in 1 g of leaves). The next was aloe gel obtained in laboratory (94.9 mg of GAEin 100 g of gel -0.9jUmol ofTrolox in 1 g of gel). The least polyphenol compounds (34.6 mg of GAE in 100 g of preparation) and the lowest antioxidant activity (0.13 jumol ofTrolox in 1 g of preparation) were in manufactured aloe gel. After calculating these values in dry mass a similar antioxidant activity of whole aloe leaf and preparations obtained experimentally (gel and skin) was determined. It was almost 4 times lower in manufactured gel.
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