Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 16

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  succinate dehydrogenase
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Histoenzymological localization of glycerol 3 phosphate dehydrogenase and succinate dehydrogenase in the mesencephalon of a minor carp Barilius bendelisis (Hamilton)

100%
Singh N.
Acta Ichthyologica et Piscatoria
|
1988
|
tom 18
|
nr 2
2

Novel yeast cell dehydrogenase activity assay in situ

100%
Berlowska J., Kregiel D., Klimek L., Orzeszyna B., Ambroziak W.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 2
The aim of this research was to develop a suitable method of succinate dehydrogenase activity assay in situ for different industrial yeast strains. For this purpose different compounds: EDTA, Triton X-100, sodium deoxycholate, digitonin, nystatin and β-mercaptoethanol were used. The permeabilization process was controlled microscopically by primuline staining. Enzyme assay was conducted in whole yeast cells with Na-succinate as substrate, phenazine methosulfate (PMS) as electron carrier and in the presence one of two different tetrazolium salts: tetrazolium blue chloride (BT) or cyanoditolyl tetrazolium chloride (CTC) reduced during the assay. In comparabile studies of yeast vitality the amount of intracellular ATP was determined according to luciferin/luciferase method. During the succinate dehydrogenase assay in intact yeast cells without permeabilization, BT formazans were partially visualized in the cells, but CTC formazans appeared to be totally extracellular or associated with the plasma membrane. Under these conditions there was no linear relationship between formazan color intensity signal and yeast cell density. From all chemical compounds tested, only digitonin was effective in membrane permeabilization without negative influence on cell morphology. Furthermore, with digitonin-treated cells a linear relationship between formazan color intensity signal and yeast cell number was noticed. Significant decreasing of succinate dehydrogenase activity and ATP content were observed during aging of the tested yeast strains.
3

Fluorek - kompetycyjny i niekompetycyjny inhibitor dehydrogenazy bursztynianowej

84%
Stachowska E., Chlubek D., Machoy Z.
Bromatologia i Chemia Toksykologiczna
|
1999
|
tom 32
|
nr 2
111-117
Zbadano typ hamowania dehydrogenazy bursztynianowej [E.C. 1.3.99.1] (SDH) przez fluorek w zmiennych warunkach doświadczenia. Badania prowadzono z preparatami cząstek submitochondrialnych, co pozwoliło śledzić zachowanie enzymu w naturalnym otoczeniu błony mitochondrialnej. Ustalono, że w zależności od warunków doświadczalnych - a więc Stężeniu fluorku i bursztynianiu w roztworze, jon fluorkowy może zachowywać się jak inhibitor kompetycyjny lub niekompetycyjny.
4

Morphohistochemical profile of red and pink muscles in freshwater fish

84%
Nejedli S., Kozaric Z., Gjurcevic Kantura V., Zobundzija M., Petrinec Z., Matasin Z., Vlahovic K.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 11
1307-1310
The morphohistochemical profile of red and pink muscles from the caudal portion of the trunk at the height of the horizontal septum in the Carassius auratus gibelio and Perca fluviatilis 20-25 cm long from the Gare.nica (Croatia) fish farm have been studied. Muscle samples, taken on arrival from live fish, were frozen in liquid nitrogen, cut on the cryo cut into 10 µm thick slices stained with hemalum and eosin and with the procedure for the detection of succinate dehydrogenase (SDH). The diameter of muscle fibers from the obtained preparations was measured using a micrometer scale with 10 µm divisions on a Nikon microscope. The red muscle of the Carassius auratus gibelio and Perca fluviatilis is known to have a triangular cut. It is positioned laterally beneath the skin and runs craniocaudally along the horizontal septum. Underneath the surface red muscle there is a pink muscle, shorter than the red one. In the Carassius auratus gibelio it is wide and divided into the surface (pink I) and deep (pink II) layer, while in Perca fluviatilis it is narrow, short and unchanged, but comprises a narrow transitional zone towards the red muscle. The red muscle is composed of red muscle fibers of small diameter with potent SDH activity. The pink muscle is composed of two types of fibers . intermediary small-diameter muscles of pronounced SDH activity and large-diameter muscles of less pronounced SDH activity as well as white muscles of large diameter with poor SDH activity. Intermediary fibers in the Carassius auratus gibelio accounted for 77.80% of the fibers in the pink I layer and 44.90% of the fibers in the pink II layer. White fibers in the same fish accounted for 22.20% of the fibers in the pink I layer and 55.10% of the fibers in the pink II layer. Intermediary fibers in the pink muscle of the Perca fluviatilis accounted for 58.80% fibers, whereas white fibers accounted for 41.20% of all fibers.
5

A pilot study on the histochemical evaluation of Na-K-ATPase and succinate dehydrogenase in rat intestinal villi

84%
Grudzinski I. P.
Roczniki Państwowego Zakładu Higieny
|
2002
|
tom 53
|
nr 1
Effects of poisoning with sodium nitrite on Na/K-ATPase and succinate dehydrogenase (SDH) activity were examined in the small intestine mucosa of male Wistar rats. The animals were treated per os with either an aqueous solution of sodium nitrite (10 mg NaNO2/kg b.w.) or normal saline (control) daily for 3 or 12 weeks. Histochemical analyses show that sodium nitrite decreased Na/K-ATPase and SDH in rat intestinal villi, and the nitrite side effect(s) was mainly observed in animals treated for 12 weeks.
6

Influence of enflurane and isoflurane on some indicatory enzymes of the cerebellar cortex

84%
Kaminski M., Plewka A., Kmieciak-Kolada K., Herman Z. S., Was M., Chmielnicki Z., Dyaczynska-Herman A.
Polish Journal of Environmental Studies
|
1995
|
tom 04
|
nr 2
Of both drugs studied only enflurane enhanced the activities of the following enzymes: succinate dehydrogenase (SD), thiamine pyrophosphatase (TPP-ase), and acid phosphatase (AcP). The activation of metabolic processes was maximal in group 3 (after 60 min anaesthesia) and group 4 (1 hr after 60 min anaesthesia), and persisted in group 5 (24 hr after 60 min anaesthesia). Furthermore, we found an increase in the activity of lysosomal enzymes and an abnormal, diffuse Mg+2-ATP-ase reaction in the perikaryons of Purkinje cells. This indicates that these cells were damaged.
7

Succinate dehydrogenase and acid phosphatase activity in Phaseolus lunatus testa

84%
Aniszewski T.
Acta Biologica Cracoviensia. Series Botanica
|
2006
|
tom 48
|
nr 2
Succinate dehydrogenase (SDH) and acid phosphatase (AcP) activity in Phaseolus lunatus seed testa are demonstrated in enzyme tests, and uptake and transport of vital and indicator dyes such as methylene blue, Congo red and tetrazolium in the seed testa are examined by light and transmission electron microscopy. SDH activity was observed in the vascular bundles (endotesta) and in some cells in endo- and mesotesta. AcP activity was located near cell walls in both meso- and endotesta. In the vascular bundles there was very little AcP activity. Vital and indicator dyes were conducted from the exotesta (hilum) to endotesta. Vesicle mobilization was observed in the mesotesta. Strong enzyme activity in the meso- and endotesta and vesicle mobilization in the mesotesta suggest the potential active role of testa strata in imbibition and the initial nutritional stage of germination.
8

Cytochemical study of dehydrogenase activity in two euglenid species of the genus Parastasia Michajlow,1966

67%
Wita I.
Acta Protozoologica
|
1989
|
tom 28
|
nr 1
9

Jon fosforanowy kompetycyjnym inhibitorem dehydrogenazy bursztynianowej

67%
Stachowska E., Machoy Z., Chlubek D.
Bromatologia i Chemia Toksykologiczna
|
2000
|
tom 33
|
nr 3
263-267
Badano zależność pomiędzy aktywnością dehydrogenazy bursztynianowej [E.C. 1.3.99.1] a jonem fosforanowym w niejonowym roztworze buforu Tris-kakodylowego. Preparatem pomiarowym były cząstki submitochondrialne, pozbawione szczawiooctanu - inhibitora związanego z centrum aktywnym enzymu. Na podstawie przeprowadzonych badań wykazano, że fosforan jest inhibitorem kompetycyjnym enzymu (przy stosunkowo niskich jak dla warunków pomiarowych stężeniach fosforanu - rzędu 10 mmoli/dm3). Uzyskane wyniki zwracają uwagę na fakt, że stosowane często do pomiaru aktywności enzymów bufory fosforanowe (gdzie stężenie fosforanu osiąga nawet 200 mmoli/dm3) mogą wpływać niekorzystnie na aktywność niektórych enzymów oddechowych.
10

Effects of niclosamide on Oncomelania hupensis, the intermediate snail host of Schistosoma japonicum: an enzyme-histochemical study

67%
Wang W., Li H., Liang Y., Dai J.
Acta Parasitologica
|
2009
|
tom 54
|
nr 2
172-179
An enzyme-histochemical study of five enzymes, namely succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), cytochrome oxidase (CCO), cholinesterase (CHE) and nitric oxide synthase (NOS), was elucidated in the soft tissues of Oncomelania hupensis, the intermediate host snail of Schistosoma japonicum, before and after the treatment with a suspension concentrate of niclosamide (SCN). Following the treatment of SCN, a marked loss occurred in the activity of the five enzymes mentioned above. LDH and SDH showed their strongest activity in the buccal mass and muscular fibers, CCO in buccal mass and liver, CHE in pellicle and ganglia, and NOS in muscular fibers and pharyngeal canal. The results indicate that SCN impairs the activities of the enzymes influencing the transfer of neurotransmitter and energy supply in O. hupensis, ultimately leading to the loss of various physiological functions, which is considered to be a cause of death in O. hupensis.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The influence of heavy metal ions on the viability and metabolic enzyme activity of the marbled crayfish Procambarus virginalis (Lyko, 2017)

67%
Marenkov O., Prychepa M., Kovalchuk J.
International Letters of Natural Sciences
|
2018
|
tom 70
The article shows the results of studies on the influence of heavy metal ions (manganese, nickel, lead) on the viability and metabolic enzyme activity of marbled crayfish Procambarus virginalis (Lyko, 2017) (Decapoda). Due to the fact that marbled crayfish got into the reservoirs of the Dnipropetrovsk region in 2015, it was necessary to study the possibilities of its adaptation to environmental factors of reservoirs for further prediction of its distribution or even acclimatization under conditions of toxicological contamination of the ponds of the steppe Prydniprovya. In the experiment with marbled crayfish, chronic effects of various concentrations of heavy metal ions on the physiological state and enzyme activity were investigated. The obtained results showed that among the investigated heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. According to the results of the research, significant changes were noted in the individual biochemical parameters of marbled crayfish under the influence of manganese, lead and nickel ions. The most significant changes in the activity of lactate dehydrogenase were detected in muscle tissues affected by manganese and nickel ions. A significant decrease in the activity of succinate dehydrogenase in muscle of marbled crayfish was determined after the action of heavy metal ions. Investigation of changes in the activity of alkaline phosphatase under the influence of the ions of manganese, lead and nickel has its own characteristics, which indicates certain violations in the tissues of cell membranes. Changes in the activity of enzymes were also reflected in the overall protein content. In conclusion, changes in these parameters may indicate a rapid biochemical response of crustaceans to the toxic effects of heavy metals.
12

Histochemical studies on Fasciola hepatica in mice. II. Oxidoreductase activity in experimentally induced fasciolosis

67%
Kolodziejczyk L.
Acta Parasitologica
|
1996
|
tom 41
|
nr 4
CFW strain white mice were infected with various doses (10, 30, 50 and 100) of Fasciola hepatica metacercariae administered per os. Using histochemical methods, the activity of selected oxidoreductases (α-glycerophosphate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, and cytochrome oxidase) was evaluated in the liver of the mice suffering from acute and chronic fasciolosis. Intensified anaerobic and aerobic respiration was recorded. The host’s compensatory mechanism observed in the form of intensified metabolism, was found to be related to the infection intensity.
13

Evaluation of the influence of milk and fat diet on morphology and histochemistry of rat liver under inhalatory exposure to ammonium fluoride aerosol

67%
Machoy-Mokrzynska A., Kolodziejczyk L.
Acta Poloniae Toxicologica
|
2001
|
tom 09
|
nr 2
14

Histochemical studies on Fasciola hepatica in mice. I. Oxidoreductase activity in juvenile and adult liver flukes

67%
Kolodziejczyk L.
Acta Parasitologica
|
1996
|
tom 41
|
nr 4
Histochemical assays were made to study the activity of the following respiratory enzymes: α-glycerophosphate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, and cytochrome oxidase in tissues of Fasciola hepatica during the parasite’s development in the liver of the definitive host. The respiratory enzyme activity was assessed in the tegument, parenchyma, oral sucker, and in the caecum of juvenile and adult F. hepatica inhabiting the host’s liver parenchyma (infection week 1 to 4) and bile ducts (infection week 5-7), respectively. Changes in the intensity of the parasite’s reaction to the dehydrogenases studied were found to be habitat-dependent (liver parenchyma vs. bile ducts). No cytochrome oxidase activity was detected in F. hepatica tissues.
15

Histochemical and enzyme-histochemical studies of Oncomelania hupensis (Gredler, 1881), the intermediate host of Schistosoma japonicum

59%
Wang W., Dai J., Li H., Liang Y.
Acta Parasitologica
|
2011
|
tom 56
|
nr 1
The distribution of glycogen, DNA and histone, and localization of activity of ten enzymes such as glucose-6-phosphatase (G-6-Pase), cytochrome oxidase (CCO), lactate dehydrogenase (LDH), 5′-nucleotidase (5′-NT), succinate dehydrogenase (SDH), glucose-6-phosphate dehydrogenase (G-6-PDH), alkaline phosphatase (ALP), acid phosphatase (ACP), Mg2+-adenosine triphosphatase (Mg2+-ATPase), and cholinesterase (CHE) in Oncomelania hupensis (Gredler, 1881) snails, the intermediate host of Schistosoma japonicum, was surveyed using the histochemical and enzyme-histochemical techniques. The results showed that the glycogen with high activity was widely distributed in snails. DNA showed its strongest reaction in testis. The histone was distributed in sites of the radula, parenchyma of penis and ovary. LDH and SDH were widely distributed in snails and all were abundant. Sites of the strongest enzyme activities of G-6-PDH were evident in the reproductive system together with central ganglia. Only the ovary showed a strong enzyme activity of G-6-Pase. Most tissues and organs contained CCO, and the activity of this enzyme was very strong. 5′-NT showed a strong enzyme activity in the ovary and testis. Mg2+-ATPase was localized in sites of the liver, stomach and reproductive gland. A strong enzyme activity of ALP appeared in the digestive system. ACP showed a low activity in snails. The central ganglia, nervous stem, liver, branchial duct, epithelia of the head and foot regions showed a very strong enzyme activity of CHE. The findings could provide a theoretical basis for development of highly effective molluscicides with low toxicity to other biota, as well as means for novel snail control strategies.
16

Oddzialywanie kadmu w zaleznosci od dawki i stopnia kumulacji na jelito cienkie ciezarnych samic szczurzych

51%
Kaminska O.
Bromatologia i Chemia Toksykologiczna
|
1995
|
tom 28
|
nr 2
161-167
Intoksykacja kadmem ciężarnych samic szczurzych od 7 do 19 dnia ciąży, w dawkach w zależności od grupy doświadczalnej równych 0,2, 2,0 oraz 20,0 mg Cd/kg masy ciała, powodowała w jelicie cienkim spadek aktywności procesów fosforylacji oksydatywnej korelujący z wielkością dawki oraz zawartością metalu w ścianie badanych odcinków jelita cienkiego. Równolegle obserwowano aktywację beztlenowych szlaków uzyskiwania energii.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.