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1

Improving the efficiency of halogenated benzimidazole inhibitors of protein kinase CK2

100%

Meggio F., Pagano M. A., Andrzejewska M., Cesaro L., Kazimierczuk Z., Pinna L. A.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2A

2

Studies on oligosaccharyl transferase in yeast

75%

Lennarz W. J.

Acta Biochimica Polonica

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2007

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tom 54

|

nr 4

673-677

In yeast, OT consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p. Four others are not essential Ost3p, Ost4p, Ost5p, Ost6p. All yeast OT subunits have been cloned and sequenced (Kelleher et al., 1992; 2003; Kelleher & Gilmore, 1997; Kumar et al., 1994; 1995; Breuer & Bause, 1995) and the structure of one of them, Ost4p, has been solved by NMR (Zubkov et al., 2004). Very recently, the preliminary crystal structure of the lumenal domain of an archaeal Stt3p homolog has been reported (Mayumi et al., 2007). Homologs of all OT subunits have been identified in higher eukaryotic organisms (Kelleher et al., 1992; 2003; Kumar et al., 1994; Kelleher & Gilmore, 1997).

3

Phosphorylation of a precursor of substance P [alpha-protachykinin] by a catalytic subunit of protein kinase A

75%

Hrabec E., Strek M., Hrabec Z.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

4

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Cyclic AMP deficiency stimulates a stress condition in tobacco BY-2 cells

63%

Sgobba A., Sabetta W., Blanco E., Paradiso A., Viggiano L., De Pinto M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 2

5

Immune signalling and vesicular trafficking – intertwining networks regulated by E3 ubiquitin ligases

63%

Stegmann M., Furlan G., Anderson R. G., Ichimura K., McDowell J. M., Shirasu K., Trujillo M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

6

Zolpidem withdrawal induced uncoupling of GABAA receptors in vitro associated with altered GABAA receptor subunit mRNA expression

63%

Jazvinscak Jembrek M., Vlainic J., Suran J.

Acta Neurobiologiae Experimentalis

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2015

|

tom 75

|

nr 2

Hypnotic Zolpidem produces its effects via the benzodiazepine binding site in a1-containing GABAA receptors. The aim of the study was to assess the influence of duration of Zolpidem treatment and its withdrawal, as well as the role of a1-containing GABAA receptors in the development of physical dependence and tolerance. Namely, recombinant receptors can be used to characterize mechanisms involved in different processes in the brain and to delineate the contribution of specific receptor subtypes. To address the influence of chronic Zolpidem treatment we exposed HEK293 cells stably expressing a102y2S recombinant GABAA receptors for seven consecutive days, while withdrawal periods lasted for 24, 48, 72 and 96 hours. Using radioligand binding studies we determined that chronic Zolpidem treatment did not induce changes in either GABAA receptor number or in the expression of subunit mRNAs. We observed the enhancement of binding sites and upregulated expression of subunit mRNAs only following 96-hour withdrawal. Moreover, Zolpidem treatment and its withdrawal (all time points) induced functional uncoupling between GABA and benzodiazepine binding sites in the GABAA receptor complex. Accordingly, it might be assumed that Zolpidem withdrawal-induced uncoupling of GABAA receptors is associated with altered GABAA receptor subunit mRNA expression. The results presented here provide an insight into molecular and cellular mechanisms probably underlying adaptive changes of GABAA receptor function in response to chronic usage and withdrawal of zolpidem and perhaps the observed molecular changes could be linked to the tolerance and dependence produced upon prolonged treatment with other GABAergic drugs.

7

Computational modeling of the bacterial 70S ribosome and its subunits

63%

Trylska J.

Biotechnologia

|

2009

|

nr 1

36-47

8

Limited proteolysis of E. coli ATP-dependent protease Lon - a unified view of the subunit architecture and characterization of isolated enzyme fragments

63%

Melnikov E. E., Andrianova A. G., Morozkin A. D., Stepnov A. A., Makhovskaya O. V., Botos I., Gustchina A., Wlodawer A., Rotanova T. V.

Acta Biochimica Polonica

|

2008

|

tom 55

|

nr 2

281-296

We carried out chymotryptic digestion of multimeric ATP-dependent Lon protease from Escherichia coli. Four regions sensitive to proteolytic digestion were located in the enzyme and several fragments corresponding to the individual structural domains of the enzyme or their combinations were isolated. It was shown that (i) unlike the known AAA+ proteins, the ATPase fragment (A) of Lon has no ATPase activity in spite of its ability to bind nucleotides, and it is monomeric in solution regardless of the presence of any effectors; (ii) the monomeric proteolytic domain (P) does not display proteolytic activity; (iii) in contrast to the inactive counterparts, the AP fragment is an oligomer and exhibits both the ATPase and proteolytic activities. However, unlike the full-length Lon, its AP fragment oligomerizes into a dimer or a tetramer only, exhibits the properties of a non-processive protease, and undergoes self-degradation upon ATP hydrolysis. These results reveal the crucial role played by the non-catalytic N fragment of Lon (including its coiled-coil region), as well as the contribution of individual domains to creation of the quaternary structure of the full-length enzyme, empowering its function as a processive protease

9

The structure of the ribosome - short history

63%

Bakowska-Zywicka K., Tyczewska A.

Biotechnologia

|

2009

|

nr 1

14-23

10

Rational design of new competitive inhibitors of human CK2 activity

63%

Moro S., Ben D. D., Zagotto G., Meggio F., Sarno S., Pinna L. A.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 2A

11

Low molecular mass products of depolymerization of purified mucin - attempts at isolation and characterization

63%

Minkiewicz I., Gindzienski A.

Acta Biochimica Polonica

|

1999

|

tom 46

|

nr 4

Samples of crude mucin were incubated at room temperature for 48 and 96 h in a sodium azide containing buffer, pH 7.0. Then each sample was purified, reduced and alkylated with iodo[14C]acetamide. Electrophoretic analysis demonstrated that radioactivity was incorporated into the mucin subunits and proteins of 100 and 140 kDa. The results of our experiments suggest that the released proteins can be a part of mucin molecule, cleaved by proteolysis and reduction of disulfide bridges.

12

Axoplasmic localisation of the NFkappaB p65 subunit in the rat brain

63%

Sulejczak D., Skup M.

Acta Neurobiologiae Experimentalis

|

2000

|

tom 60

|

nr 2

13

cAMP-dependent protein kinase: dynamics of catalysis and docking

63%

Taylor S. S., Akamine P., Yang J., Wu J., Iyer G., Anand G., Valiev M., Weare J. H., Johnson D. D.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 2A

14

Crystal structure of an altered catalytic subunit of cAMP-dependent protein kinase in complex with the PKC-kinase inhibitor bisindolyl-maleimide 2 in two different conformations - implications for selectivity

63%

Gabel M., Breitenlechner C. B., Engh R. A., Bossemeyer D.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 2A

15

Anti-peptide specific antibodies for the characterization of different glutamate dehydrogenase subunits in yellow lupine

63%

Lehmann T., Dabert M., Ratajczak L.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

16

Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima

51%

Huang Z., Long H., Jiang Q.-T., Wei Y.-M., Yan Z.-H., Zheng Y.-L.

Journal of Applied Genetics

|

2010

|

tom 51

|

nr 1

9-18

Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the В genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the В genome, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene famailies. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat.

17

Expression of N-methyl-D-aspartate (R)(GluN2B) - subunits in the brain structures of rats selected for low and high anxiety

51%

Lehner M., Wislowska-Stanek A., Skorzewska A., Maciejak P., Szyndler J., Turzynska D., Sobolewska A., Krzascik P., Plaznik A.

Journal of Physiology and Pharmacology

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2011

|

tom 62

|

nr 4

18

Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers

51%

Moczulski M., Salmanowicz B. P.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 4

Ograniczanie wyników

Improving the efficiency of halogenated benzimidazole inhibitors of protein kinase CK2

Studies on oligosaccharyl transferase in yeast

Phosphorylation of a precursor of substance P [alpha-protachykinin] by a catalytic subunit of protein kinase A

Cyclic AMP deficiency stimulates a stress condition in tobacco BY-2 cells

Immune signalling and vesicular trafficking – intertwining networks regulated by E3 ubiquitin ligases

Zolpidem withdrawal induced uncoupling of GABAA receptors in vitro associated with altered GABAA receptor subunit mRNA expression

Computational modeling of the bacterial 70S ribosome and its subunits

Limited proteolysis of E. coli ATP-dependent protease Lon - a unified view of the subunit architecture and characterization of isolated enzyme fragments

The structure of the ribosome - short history

Rational design of new competitive inhibitors of human CK2 activity

Low molecular mass products of depolymerization of purified mucin - attempts at isolation and characterization

Axoplasmic localisation of the NFkappaB p65 subunit in the rat brain

cAMP-dependent protein kinase: dynamics of catalysis and docking

Crystal structure of an altered catalytic subunit of cAMP-dependent protein kinase in complex with the PKC-kinase inhibitor bisindolyl-maleimide 2 in two different conformations - implications for selectivity

Anti-peptide specific antibodies for the characterization of different glutamate dehydrogenase subunits in yellow lupine

Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima

Expression of N-methyl-D-aspartate (R)(GluN2B) - subunits in the brain structures of rats selected for low and high anxiety

Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers