Wyniki wyszukiwania

1

Uptake and subcellular distribution of intraventricularly injected [1-3H]dolichol in rat brain

100%

Jankowski W., Strosznajder J.

Acta Biochimica Polonica

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1992

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tom 39

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nr 2

The uptake of C95[l-3H]dolichol in the form of liposomes into rat brain after the intracerebral and intraperitoneal injection was investigated. Efficient, time-dependent uptake of dolichol into the brain was observed exclusively after the intraventricular injection. Within 24 h after the injection about 10% of the applied dolichol was found in the brain and 1.5% in liver. The distribution of dolichol in various parts of rat brain decreased in the order: cerebellum > midbrain > grey matter and brain stem > white matter. Sevtn days after the injection total radioactivity in the brain decreased and concomitantly a significant increase was observed in blood circulating and liver of the rat. The highest activity was found in grey matter and it remained a few times higher in comparison with that in whitematter. About 80% of the dolichol taken up by the brain membrane was recovered in the following subcellular fractions: crude nuclear fraction > microsomes > mitochondria > synaptosomes > myelin.

2

The toxicity of cerium nitrate to Elodea canadensis: subcellular distribution, chemical forms and physiological effects

86%

Chu W. Y., Cai S. J., Fu Y. Y., Li F. F., Xu T., Qiu H., Xu Q. S.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

The rare earth elements are increasingly being used as trace supplements in different fields. In this study, subcellular distribution, the chemical forms and toxicity of cerium (Ce) were evaluated for Elodea canadensis. The effect of Ce (5–20 mg L⁻¹) applied for 7 days was assessed by measuring changes in the nutrient elements, photosynthetic pigments, malondialdehyde and antioxidant systems. Ce accumulation was greatest in the cell walls, followed by the organelles and the soluble fraction. Ce levels were higher in cellulose and pectin than in other biomacromolecules. The toxic effects caused by Ce were shown by a reduction in photosynthetic pigments, disruption of nutrient elements, and increases in MDA content. E. canadensis shows Ce-induced oxidative stress by modulating antioxidant enzymes, such as guaiacol peroxidase and catalase. Elevated Ce levels may represent a potential risk for aquatic ecosystems.

3

Subcellular distribution of alanine aminotransferase activity in maize [Zea mays L.] leaves

86%

Orzechowski S., Socha-Hanc J., Paszkowski A.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 4

4

The subcellular distribution of the p53 tumour suppressor, and organismal ageing

86%

Wesierska-Gadek J., Schmid G.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 3

5

Organisation and composition of the plant nuclear matrix. Characterisation and subcellular distribution of a MAR-binding protein: AcMFP1

86%

Samaniego R., Diaz de la Espina S.M.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

6

Gelsolin in human colon adenocarcinoma cells with different metastatic potential

72%

Litwin M., Mazur A. J., Nowak D., Mannherz H. G., Malicka-Blaszkiewicz M.

Acta Biochimica Polonica

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2009

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tom 56

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nr 4

739-743

Gelsolin, one of a major actin-binding proteins, is involved in the regulation of actin cytoskeleton organization by its severing and capping activity towards actin filaments. Human colon adenocarcinoma cell line LS180 and its selected variants of different metastatic potential were used to check for a correlation between gelsolin level, its subcellular localization and the invasive capacity of cells. Based on immunoblotting experiments, a decreased level of gelsolin was detected in the most invasive 5W subline when compared to the parental cell line LS180. The intracellular distribution of actin filaments and gelsolin in colon adenocarcinoma cells was examined by confocal microscopy. In the 5W subline, unlike in the other examined cells, gelsolin was colocalized with filamentous actin at the cell periphery. In summary, in human colon adenocarcinoma cells, gelsolin level and its subcellular distribution seem to correlate with their metastatic potential.

7

Brain spectrin and friends

72%

Riederer B. M., Wintergerst E. S., Valcourt E., Routtenberg A.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 1

Brain spectrin (fodrin, calspectin), is an actin binding protein, and composed of two α- and two β-subunits which combine by head-to-head interaction to a heterotetramere. Together with other proteins it forms a proteinaceous meshwork underlying the cytoplasmic surface of the plasma membrane. In the mammalian brain, three forms of spectrins were identified, an axonal, a somato-dendritic and a astroglial one. A subcellular localization at the electron microscopic-level suggests that these forms are not only attached to membranes but expand into the cytoplasm. It was shown that they differ in their subcellular distribution, and their temporal appearance during postnatal brain development. The subplasmalemmal cytoskeleton is composed of brain spectrin and a variety of proteins such as actin and calmodulin, and we discuss here two additional proteins, FI and parvalbumin, which may bind to brain spectrin and may have to be included in the circle of proteins interacting with brain spectrin.

8

The differences in physiological responses, ultrastructure changes, and Na plus subcellular distribution under salt stress among the barley genotypes differing in salt tolerance

58%

Jabeen Z., Hussain N., Han Y., Shah M. J., Zeng F., Zeng J., Zhang G.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

Plants adopt several strategies to maintain cellular ion homeostasis, including physiological, biochemical, cellular, subcellular, and molecular mechanisms for fighting against salt stress. We investigated the responses of tolerant Tibetan wild barley (XZ16), tolerant (CM72) and sensitive (Gairdner) barley cultivars at physiological, cellular, and molecular levels. The results revealed that salinity induced a significantly greater reduction in total root length, surface area, diameter, and total volume in Gairdner than in CM72 and XZ16. Analysis of gene expression using quantitative RT-PCR showed that transcripts of vacuolar H⁺-ATPase and inorganic pyrophosphatase (HvHVA/68 and HvHVP1) were more abundant in leaves and roots of XZ16 and CM72 than those of Gairdner. Observation of electron microscopy detected the difference in the damage of leaf and root ultrastructure among the three genotypes under salt stress, with XZ16 and Gairdner being least and most affected, respectively. Subcellular study showed that a primary strategy to protect the cytosol against sodium toxicity was compartmentalization of sodium ions into soluble fraction (vacuoles). Gairdner showed drastically stronger sodium-specific fluorescence visualized by CoroNa-Green, a sodium-specific fluorophore, than CM72 and XZ16.

9

Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana

58%

Lesicka-Gorecka J., Szarzynska B., Sawczak M., Bagdiul I., Gorski P., Jarmolowski A., Szweykowska-Kulinska Z.

Acta Biochimica Polonica

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2008

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tom 55

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nr 3

517-524

HYL1 is a nuclear protein involved in the processing of miRNAs but its exact function remains unknown. Arabidopsis thalianahyl1mutants exhibit hypersensitivity to ABA. We decided to answer the question whether ABA affects the HYL1 protein localization within the cell and show that it does not. We also studied the expression of HYL1 in different tissues and organs. In this paper we show for the first time the expression profile of the HYL1 protein using anti-HYL1 antibodies. The protein is present in seedlings and mature plants in all organs studied, with the highest amount in inflorescences. A. thalianaHYL1 protein has several repetitions of a 28-amino-acid sequence at the C-terminus that confer protein instability. Our bioinformatic analysis of HYL1 homologs in different Brassicaspecies shows that this repetition is typical only for Arabidopsis. This may suggest a relatively late evolutionary acquisition of the C-terminal domain.

Ograniczanie wyników

Uptake and subcellular distribution of intraventricularly injected [1-3H]dolichol in rat brain

The toxicity of cerium nitrate to Elodea canadensis: subcellular distribution, chemical forms and physiological effects

Subcellular distribution of alanine aminotransferase activity in maize [Zea mays L.] leaves

The subcellular distribution of the p53 tumour suppressor, and organismal ageing

Organisation and composition of the plant nuclear matrix. Characterisation and subcellular distribution of a MAR-binding protein: AcMFP1

Gelsolin in human colon adenocarcinoma cells with different metastatic potential

Brain spectrin and friends

The differences in physiological responses, ultrastructure changes, and Na plus subcellular distribution under salt stress among the barley genotypes differing in salt tolerance

Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana