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Background. Enormous diversities exist regarding shape, number, and arrangement of the olfactory lamellae, distribution of the sensory and non-sensory epithelium as well as the abundance of various receptor cells among different teleosts. This study was aimed at describing the morphology, histology and SEM ultrastructure of the olfactory epithelium of a spotted snakehead fish, Channa punctatus (Bloch, 1793). Materials and Methods. OE of C. punctatus was examined through LM and SEM. Results. Olfactory epithelium of C. punctatus containing 18 to 20 lamellae provides a large surface area. Sensory epithelium possesses both ciliated and microvillous olfactory receptor cells (ORC), which are intermingled and distributed throughout the epithelium. ORCs are arranged in alternate rows with supporting cells (SCs). ORC is columnar cell with a round cell body, an axonal process and a long dendrite. The apical surfaces of the ORCs are provided either with 10–12 cilia or numerous microvilli and the SCs with fingerprint like microridges. ORCs with rod-shaped dendrite ending were found in small numbers. Non-sensory epithelium is composed of ciliated nonsensory cells (cNSC) and covered with a dense carpet of cilia, which help in ventilating the olfactory chambers. Conclusion. A large surface area of OE, the high density of both ciliated and microvillous ORC and adequate arrangement for ventilating the olfactory chambers indicate that C. punctatus is very much dependent on its olfactory sense.
The structural organisation of tunica intima in the aorta is important for its integrity, prediction, and diagnosis of atherosclerosis. The goat is a suitable model for cardiovascular studies, but the structure of its tunica intima is scarcely reported. This study, therefore, aimed to describe features of the goat aortic tunica intima by light and transmission electron microscopy. Sixteen healthy male domestic goats (capra hircus) aged between 6 and 24 months were used: 8 for light and 8 for electron microscopy. The animals were euthanised with sodium pentabarbitone 20 mg/mL and fixed with 3% phosphate buffered glutaraldehyde. For light microscopy, specimens from various regions of the aorta were routinely processed for paraffin embedding and 7 μm sections stained with Mason’s trichrome. Those for transmission electron microscopy were post fixed in osmium tetroxide, embedded in Durcupan, and ultrathin sections stained with uranyl acetate and counter stained with lead citrate. Endothelium comprises round and squamous cells, linked to the subendothelial material by a simple and sometimes lamellated basement membrane. In the subendothelial zone, a heterogenous population of cells are connected with interlinked collagen and elastic fibres. Both cells and fibres are connected to the internal elastic lamina. The composite structure and interlinkages in the tunica intima permit unitary function and increase mechanical strength, thus enabling it to withstand haemodynamic stress. (Folia Morphol 2010; 69, 3: 164–169)
The fine anatomical structures of the olfactory epithelium of Cyprinus carpio (Linnaeus) have been systematically studied with the help of the scanning electron microscope (SEM). The olfactory rosette is an oval structure composed of a number of lamellae arranged on a median raphe. A large part of the lateral surface of the rosette is covered with non-receptor epithelium, whereas the receptor epithelium occupies a much smaller area in the middle part. The nonreceptor epithelium is covered with a tuft of ciliated supporting cells, among which the stratified epithelial cells and mucous cells are located. The receptor epithelium is represented by the flagellated and microvillus receptor and supporting cells. Different cells on the olfactory epithelium correlate with the functional significance of the fish concerned.
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