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This work aimed to underline the dynamics of photosynthetic and oxidative stress parameters of ‘Matador’ and ‘Andromeda’ spinach species after short-term 1 and 2 kJm–2 UV-B radiation effect. When plants reached 3–4 leaves growths stage, the exposure to 1 kJm–2 and 2 kJm–2 UV-B radiation was done once for 68 and 136 minutes, respectively. The photosynthetic and oxidative stress parameters were determined 2, 24, 48 and 72 hours after exposure. The stimulating effect of UV-B emerged on the 3rd day after exposure. The positive effect of UV-B was more pronounced for ‘Matador’. The highest DPPH radical-scavenging capacity and the highest concentration of α-tocopherols were detected 24 hours after 2 kJ UV-B exposure, but the decrease in photosynthetic rate was the highest as well. Meanwhile, on the 3rd day after 1 kJ UV-B exposure, the indicators of oxidative stress of ‘Matador’ decreased, and the photosynthetic rate increased. This study highlights that low UV-B radiation acts as an eustress, by awaking positive changes in photosynthetic and oxidative stress parameters of spinach.
The erythrocytes of 12-month old Sod1 −/− mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 −/− mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 +/+ and Sod1 +/− mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme.
In the current study the influence of single compounds and concurrent exposure to popular insecticides: organophosphate (OP) – chlorpyrifos (CPF) and synthetic pyrethroid (PYR ) – α-cypermethrin (CM) on some oxidative stress parameters and cholinesterase (ChE) activity in rats was investigated. Animals received by gavage 10 mg of single compounds or 5 mg of each per kg bw daily in rapeseed oil for 14 and 28 days. Concentrations of total thiols and TBARS, activity of catalase and cholinesterase were measured in tissues. Total thiol concentrations declined in plasma in all experimental groups after 14 and 28 days, while in liver a decrease was noted after only 14 days in animals receiving CPF and after 28 days in rats treated with CM alone with a mixture of pesticides. Lipid peroxidation presented as TBARS concentration was elevated mostly after 2 weeks of exposure in brain and liver but not in plasma in all experimental groups. Catalase activity increased in erythrocytes in all groups treated with insecticides, while in liver CM administered alone reduced the activity of the enzyme. Cholinesterase was markedly depressed to a different degree in plasma and brain of animals receiving CPF alone or in combination, while CM did not significantly elevate brain ChE. The results of this study seem to indicate that CM and CPF apart from known modes of action demonstrate their toxicity also through free radical mediated mechanisms. It is also evident that CM administered with CPF does not affect the cholinesterase inhibition generated by the latter.
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