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The purpose of the study was to determine the concentration of colostral immunoglobulins, crude protein and certain electrolytes in the colostrum of the mare at different times after parturition. Relationships among the results obtained and the foals’ maturity and health were evaluated. Colostral samples were collected from 40 mares at first suckle and afer 6, 12 and 24 hours of foaling. The frozen samples were stored for 3 to 4 months and then thawed to evaluate the colostral specific gravity (g/ml). In 10 dams colostral specific gravity was measured immediately after collection and then the concentration of crude protein, colostral immunoglobulins, potassium, sodium and calcium were determined. The highest colostral specific gravity as well as concentrations of colostral immunoglobulins and crude protein were found at first suckle. Aferwards the values decreased and 12 to 24 hours after parturition the specific gravity was close to its value measured in the milk of a mare, and averaged 1.039. The colostral specific gravity in younger (4-9-years-old) mares was on the average 0.01 g/ml higher than in the older ones (10-16-years-old). Colostral specific gravities significantly correlate (r=0.90) with colostral immunoglobulin concentrations and crude protein. The concentrations of calcium and potassium were maintained on the same level whereas that of sodium decreased. At first suckle the sodium concentration was 29.2±4.4, 12 hours later it dropped to 20.56±4.9 and 24 hours after parturition it decreased to 17.75±2.8 mmol/1. Observations carried out during parturition and clinical evaluation of the foals’ maturity and health proved the physiological course of parturition.
Analysis of sIgA concentrations in the contents of the cervical canal of the uterus and of the oral cavily in women with Candida or without fungi in ontocenoses of these organs. The aim of the study was to search for fungi in ontocenoses of genital organs and oral cavity (the fungal reservoir for multifocal infections) in women; evaluation of the concentration of sIgA in the contents of the cervical canal of the uterus and of the oral cavity. 102 women (age: 18- 35 years) were examined. Fungi were isolated from ontocenoses of the vagina and the oral cavity; axenic strains were differentiated with API 20 C and API 20 C AUX tests (bioMerieux). The concentrations of sIgA in the content of the cervical canal of the uterus and from the oral cavity were evaluated by LC-Partigen IgA (Behring) tests. Candida occurence in the oral cavity was significant (p<0,02) higher than in the vagina. Candida albicans (6 codes) was the predominat species; there were also C. tropicalis, C. kefyr, C. krusei, C. guilliermondii and C. glabrata. There were no significant differences between sIgA concentrations and the presence or absence of fungi in the vagina or oral cavity.
The aim of the study was to determine IgG (total) concentrations in milk from cows with different forms of mastitis. In 51 samples of milk from black-white breed cows (15 from healthy udders, 12 from subclinical aseptic mastitis, 14 from subclinical septic mastitis and 10 from clinical mastitis) the level of IgG were measured by single radial immunodiffusion. The concentrations of IgG in mammary lacteal secretions from quarters with clinical mastitis (1477,8 mg/l) were significantly higher (p≤0,05) than in the control milk (604,6 mg/l) and in milk from subclinical aseptic (776,9 mg/l) and septic (850,1 mg/l) mastitis. There were no significant differences in concentrations of IgG in milk from aseptic and septic subclinical mastitis.
The objective of the studies was to determine the dynamics of certain parameters of nonspecific cellular and humoral immunity in piglets in the post-natal period. The parameters of immune responses were determined in blood samples of 7 randomly chosen piglets before feeding them their first doses of colostrum, 48 h after colostrum feeding and at the age of 7, 14, 21 and 35 days of life. The following parameters were examined: the NBT, phagocytic index, test of intracellular killing, level of total protein and γ-globulin complex, lysozyme activity and IgG concentration. It was found that the nonspecific mechanisms of cellular and humoral immunity increased along with the age of piglets. Moreover, the serum IgG level diminished quickly from 30 g/l at the age of 48 h to 3,7 g/l at the age 35 days. The results of examination indicate that piglets are the most sensitive to diseases between their first 14 to 35 days of life.
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