Wyniki wyszukiwania

1

Recognition of the 5' splice site by the spliceosome

100%

Konarska M. M.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 4

The splicing of nuclear pre-mRNAs is catalyzed by a large, multicomponent ribonucleoprotein complex termed the spliceosome. Elucidation of the molecular mechanism of splicing identified small nuclear RNAs (snRNAs) as important components of the spliceosome, which, by analogy to the self-splicing group II introns, are implicated in formation of the catalytic center. In particular, the 5' splice site (5'SS) and the branch site, which represent the two substrates for the first step of splicing, are first recognized by U1 and U2 snRNPs, respectively. This initial recognition of splice sites is responsible for the global definition of exons and introns, and represents the primary target for regulation of splicing. Subsequently, pairing interaction between the 5'SS and U1 snRNA is disrupted and replaced by a new interaction of the 5'SS with U6 snRNA. The 5'SS signal contains an invariant GU dinucleotide present at the 5' end of nearly all known introns, however, the mechanism by which the spliceosome recognizes this element is not known. We have identified and characterized a specific UV light-induced crosslink formed between the 5'SS RNA and hPrp8, a protein component of U5 snRNP in the spliceosome that is likely to reflect a specific recognition of the GU dinucleotide for splicing. Because recognition of the 5'SS must be linked to formation of the catalytic site, the identification of a specific and direct interaction between the 5'SS and Prp8 has significant implications for the role of this protein in the mechanism of mRNA splicing.

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The crosstalk between splicing of protein-coding genes and processing of microRNAs located within their introns

84%

Skorupa K., Sobkowiak L., Jarmolowski A., Szweykowska-Kulinska Z.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 3

3

Sklejanie na styk fornirow w metodzie poprzecznej jest nadal poprawiane

67%

W K.

Biuletyn Informacyjny Ośrodka Badawczo-Rozwojowego Przemysłu Płyt Drewnopochodnych w Czarnej Wodzie

|

1994

|

tom 35

|

nr 3

237

Ograniczanie wyników

1 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Biuletyn Informacyjny Ośrodka Badawczo-Rozwojowego Przemysłu Płyt Drewnopochodnych w Czarnej Wodzie

1 Jarmolowski A.

1 Konarska M. M.

1 Skorupa K.

1 Sobkowiak L.

1 Szweykowska-Kulinska Z.

1 W K.

1 2013

1 1998

1 1994

Recognition of the 5' splice site by the spliceosome

The crosstalk between splicing of protein-coding genes and processing of microRNAs located within their introns

Sklejanie na styk fornirow w metodzie poprzecznej jest nadal poprawiane