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Public and private EST (Expressed Sequence Tag) programs provide access to a large number of ESTs from a number of plant species, including Arabidopsis, corn, soybean, rice, wheat. In addition to the homology of each EST to genes in GenBank, information about homology to all other ESTs in the data base can be obtained. To estimate expression levels of genes represented in the DuPont EST data base we count the number of times each gene has been seen in different cDNA libraries, from different tissues, developmental stages or induction conditions. This quantitation of message levels is quite accurate for highly expressed messages and, unlike conventional Northern blots, allows comparison of expression levels between different genes. Lists of most highly expresses genes in different libraries can be compiled. Also, if EST data is available for cDNA libraries derived from different developmental stages, gene expression profiles across development can be assembled. We present an example of such a profile for soybean seed development. Gene expression data obtained from Electronic Northern analysis can be confirmed and extended beyond the realm of highly expressed genes by using high density DNA arrays. The ESTs identified as interesting can be arrayed on nylon or glass and probed with total labeled cDNA first strand from the tissue of interest. Two-color fluorescent labeling allows accurate mRNA ratio measurements. We are currently using the DNA array technology to study chemical induction of gene expression and the biosynthesis of oil, carbohydrate and protein in developing seeds. In the future, high density DNA arrays containing the whole gene complement of an organism will become available for the analysis of genome-wide patterns of gene expression.
Soybean cultivation is becoming more and more popular in many countries, including Poland, due to the high nutritional value of its seeds, its resistance to diseases and pests, as well as lower propensity to lodging compared to other legumes. Under the climatic conditions of Poland, soybean yield is largely dependent on weather conditions in a particular growing season, primarily temperature and rainfall. Studies on the effect of tillage systems used for soybean cropping under the climatic conditions of Poland are scarce. This proves the need to continue such research. The aim of this study was to compare the effect of conventional tillage (CT) and no-tillage (NT) on yield, weed infestation and seed qualitative composition of the ‘Mazowia’ soybean cultivar grown under the conditions of the Lublin Upland. A field experiment was carried out in the period of 2009-2012 at the Czesławice Experimental Farm (51°18′23″N 22°16′2″E). In 2010 the soybean plantation was terminated due to adverse weather conditions and related inhibited plant emergence. The experiment was set up on loess-derived grey-brown podzolic soil as a split-block design in four replicates. The present study showed that the soybean seed yield obtained under conventional tillage conditions was higher by 14.6% compared to that obtained under no-tillage. A higher plant density after emergence and before harvest as well as a higher plant height were found in the conventional tillage treatment. The protein, oil and fibre content in soybean seed was significantly affected only by weather conditions in particular growing seasons. A higher weed weight and richer floristic composition of weeds were found under no-tillage conditions in relation to conventional tillage.
Biological nitrogen fixation (BNF) is the process that provides organic nitrogenous compounds to the plants by using molecular nitrogen in atmosphere. Higher plants are not capable to use molecular nitrogen in atmosphere as a nitrogen source to generate essential proteins. Therefore plants either should be fertilized by adequate nitrogenous fertilizers or the microorganisms which are capable to produce nitrogenase should provide nitrogen to the plants by BNF. From among a number of factors affecting BNF, soil moisture content and ambient temperatures are considerably effective on the fixation rate. Therefore the global warming would be dramatically defective on BNF, thus effects of soil moisture as well as soil and ambient temperatures on BNF should evaluate prior rising temperature. A pot experiment was carried out to determine the effects of soil water contents on BNF. Four different soil water contents (%25, %50, %75 and %100 of water holding capacity) were adjusted either every 3 days or just after plants indicate wilting point. Non-inoculated pots were added to experiment as a control. The results revealed that BNF is affected by different level of soil water content. The mechanism of this effect would not be the direct effect of water, but the side effect of water on soil oxygen content; therefore, an aeration capability.
Between 2014 and 2020 there have been substantial rearrangements within the Common Agricultural Policy, which – in harmony with the former reform phases of CAP – has aimed to implement real conceptual and technical changes in the system of direct subsidies and market measures. As a result of this process, the system of subsidies coupled to production could be extended in Hungary, too. This new approach, which was totally different from the previous reform phases, does not restrict, but rather expands the possible amount and areas of implementation. This, in many cases, may result in a number of favourable impacts for the beneficiary sectors. Sectors, which have not received any EU subsidies before, can be supported and the member states have substantial say in forming the conditions according to their needs. In general, it is still a question, what effects can be expected, what actual consequences of the measures can be quantified on the basis of the currently available information. Following these, the main goal of this paper is to characterize the consequences of the coupled support payment by the soy production.
Progress, which is brought by new advances in modern molecular biology, allowed interference in the genome of live organisms and gene manipulation. Introducing new genes to the recipient organism enables to give them new features, absent before. Continuous increase in the area of the biotech crops triggers continuous discussion about safety of genetically modified (GM) crops, including food and feed derived from them. Important issue connected with cultivation of genetically modified crops is a horizontal gene transfer and a bacterial antibiotic resistance. Discussion about safety of GM crops concerns also food allergies caused by eating genetically modified food. The problem of genetic modifications of GM crops used for livestock feeding is widely discussed, taking into account Polish feed law.
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