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The effect of somatic cell count on the amount of daily milk yield and chemical composition of milk from 662 cows kept on 25 farms located in the region of southern Podlasie was analysed.Atotal of 5061 milk samples were evaluated. They were divided into four classes corresponding to the content of somatic cells in 1 ml of milk: <200,000; 201,000–400,000; 401,000–1,000,000; >1,000,000. It was found that, with an increase in the somatic cell count in 1 ml of milk, there was an increase in milk protein content from 3.36% to 3.54% and milk fat content from 4.02% to 4.16%, whereas the content of casein and dry matter in milk decreased from 2.56% to 2.45% and from 13.13% to 12.98%, respectively. Moreover, the percentage of casein in milk protein decreased from 76.2% to 69.2%. Also, milk yield was reduced, and the calculated losses in the milk yield resulting from an increase in the SCC were 10.6 to 17.3%.
Relationships between milk β-carotene concentrations and the cytological quality of cow’s milk. The objective of the study was to describe the relationship between milk β-carotene concentration and the cytological quality of milk of high yielding cows. The experiment was carried out at the research dairy farm of the Warsaw University of Life Sciences (WULS). From a herd of 320 Polish Holstein Friesian cows maintained in a free–stall dairy shed 58 cows were selected taking into consideration the stage of lactation (15±14 days). Cows were fed a total mixed ration (TMR) diet provided ad libitum. Four groups of cows taking into consideration concentration of β–carotene in milk has been created: 1) <150 mg L-1; 2) 151-250 mg L-1; 3) 251-450 mg L-1; 4) > 450 mg L-1. The content of β–carotene and SCC amounted at the herd level: 192 tys ml-1 and 0.312 mgL -1 respectively. The β–carotene content ranged from 0.150 to 0.451 mgL-1. The highest content of β–carotene, 0.451 mgL-1, was found in milk of cows with the lowest level of somatic cells count (SCC). The lowest level, 0.150 mgL-1, was found in milk of cows with the highest level of SCC. Milk SCC varies significantly with the concentration of β-carotene in cow’s milk. Researchers should consider monitoring for this variable as potential cofounder when exploring the relationship between mastitis, intramammary infection and nutritional management.
The aim of this study was to analyse the association between factors characterising dairy herd management and the levels of somatic cell count (SCC), and total microorganisms count (TMC) in bulk tank milk. The investigations were carried out in 2005 in 187 family farms (herd size from 6 to 60 cows), situated in adjacent parts of Warmia & Mazury, and Mazowsze regions. Based on annual geometric means, all the farms were divided into those with low and elevated SCC level (<200,000 vs. 200,000–400,000 cells/mL), and with low and elevated TMC level in milk (<50,000 vs. 50,000–100,000 cfu/mL). The milk was characterised by low SCC level in 55 farms (29.4%), whereas TMC level was low in 65 farms (34.8%). The following factors were associated with the low level of SCC: small herd size, tie-stall system of cow housing, grazing cows, use of advisory service, use of antibiotics at cow dry-off, dry storing of milking clusters between milkings, post-milking teat dipping, regular use of California Mastitis Test, treatment of clinical cases of mastitis with antibiotics in selected cows only, use of whole straw in cow feeding, and use of salt-licks containing microminerals. Factors associated with the low level of TMC were: large herd size, colostrum feeding to the newborn calf by bucket, individual housing of replacement heifers older than 3 months, pipe-line system of milking, dry storing of milking clusters between milkings, pre-milking udder and teat cleaning with wet towel soaked with a disinfectant, tank system of milk cooling and storage after milking, and regular calculation of feeding rations for cows.
The aim of this study was to evaluate the effects of somatic cell count and the polymorphic form of β4-defensin on the concentration of free fatty acids (FFA) and physico-chemical characteristics of cow’s milk. The study was carried out on 120 Polish Holstein-Friesian Black and White dairy cows.The animals were maintained in a loose barn and fed with the TMR system according to the INRA norm. The animals were divided into groups according to their polymorphic form of the defensin β4 gene: 1st – CT (def-1); 2nd – CC (def-2) and into two groups in terms of their somatic cell count:1st - <3×105 (SCC-1) and 2nd – 3×105 – 6×105 (SCC-2) cell/ml. Milk samples were collected once a month during the whole lactation. Chemical composition and some physico-chemical parameters of milk were determined by automated infrared analysis with a Milkoscan FT2 instrument. SCC were evaluated using BactoCaunt IBCm. A relationship was found between polymorphic forms of the defensin gene and the level of FFA in milk directly after milking (CT
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Wplyw stopnia zabrudzenia krow na jakosc mleka

72%
Celem pracy było określenie wpływu stopnia zabrudzenia krów na liczbę komórek somatycznych w mleku. Spośród ocenianych partii ciała 64 krów najmniejszym zabrudzeniem charakteryzowało się podbrzusze (1,2 pkt.), a następnie wymię – 1,76 pkt. Największy udział krów wykazywał lekkie zabrudzenie – 40,4%, niewiele mniej – 38,6% stanowiły zwierzęta czyste, 14,8% brudne, a tylko 6,3% krowy bardzo brudne. Wymię u 46,7% krów oceniono jako czyste, u 35% krów jako lekko zabrudzone, a u 18,3% jako brudne i bardzo brudne. Wraz ze wzrostem stopnia zabrudzenia poszczególnych partii ciała krów wzrastała liczba komórek somatycznych w mleku.
The main aim of this study was to evaluate the effect of the lactation stage (SL) on the fatty acids (FA) profile of raw milk of Brown Short-haired goats reared under organic regime. An integraf part of the study was also to assess the effect of the SL on the physico-chemical characteristics and somatic cell count (SCC) of milk. Milk records and samplings of each goat were carried out seven times from April to October. SL had a significant effect on contents of almost all monitored physicochemical properties, somatic cell counts (SCC) and FA of milk and also on average daily milk yield (DMY). DMY gradually decreased with advanced lactation (from 3.44 to 1.44 litre), whereas the content of total solids (TS) increased in the course of lactation (from 10.9 to 14.0%). Also the content of milk fat (F) increased in the course of lactation (from 3.2 to 4.7%). Contents of total protein (TP) and casein (C) were relatively high in early lactation, decreased as lactation peaked and increased towards to late lactation (3.7% of TP and 2.6% of C). Titratable acidity (TA) gradually increased from 90th day (6.2°SH) to the end of lactation (8.5°SH), while positive correlations with TS, F, TP,C and lactose were found. SCC increased as lactation advanced, moreover, a positive correlation among SCC and TA and TS was found. The SL had a significant effect on all FA groups. PUFA gradually decreased towards to late lactation, which was caused mainly by the content of linoleic acid. Similarly, the presence of linolenic acid and PUFA/SFA ratio showed a decreasing tendency with advanced lactation. Also the CLA content was the lowest at the end of lactation. PUFA n-6/n-3 ratio increased in mid lactation (7.8) and hereafter decreased towards to late lactation (3.5). In our opinion, a less favourable presence of particular groups of FA in late lactation was related with a decrease in pasture quality under organic conditions.
Despite the fact that cholesterol is a comparatively stable component of cows’ milk its concentration is, within a certain range, subject to significant variation related to the season (probably the feeding system), lactation stage and somatic cell count in milk. The highest differences (about 25%) in the amount of cholesterol per g milk fat were observed between the first and last lactation stage. Despite the decreasing milk yield with the progress of lactation, the amount of cholesterol secreted with milk increased significantly. In the milk of cows for which the somatic cell count was below 100 thousand/ml the cholesterol content was by about 10% lower than that in milk characterized by a higher somatic cell count.The positive correlation coefficients obtained between the amount of cholesterol expressed as mg/100 ml milk and the per cent of fat and protein indicate that selection conducted for increasing the concentration of nutritive components in milk will result in an increased cholesterol content.However, the quantity of cholesterol per 1 g milk fat will decrease. There was observed no correlation between the content of cholesterol in milk and the polymorphic forms of LGB.
The aim of this study was to compare the concentrations of two acute phase proteins, haptoglobin (Hp) and serum amyloid A (SAA), in serum and milk of Holstein-Friesian dairy cows grouped according to somatic cell count (SCC) thresholds (<100,000; <400,000; >400,000 cells/ml) in composite milk samples. The SCC was assessed quantitatively by FOSSOMATIC 90 analyser, serum and milk Hp and SAA concentrations were determined using commercial ELISA kits (Tridelta Development, Ltd., Wicklow, Ireland). We have found significantly higher Hp and SAA concentrations in the group with SCC >400,000 cells/ml compared to groups with lower SCC thresholds in composite milk samples. In contrast, significant differences were not recorded between the groups in serum Hp and SAA concentrations. The concentrations of Hp in serum and milk were found to be correlated (r = 0.69, P = 0.0003), whereas there was a lower correlation trend in the case of serum versus milk concentrations of SAA (r = 0.43, P = 0.0478). High significant correlations were observed either between milk haptoglobin values and SCC or milk serum amyloid A concentrations and SCC (r = 0.83, r = 0.81, P<0.0001; respectively). An increase in SCC in cows suffereing from mastitis has been accompained by strong elevation of the milk Hp and SAA, significantly correlated with serum Hp (r = 0.64, P = 0.0014) and poorly correlated with SAA values determined in serum (r = 0.43, P = 0.0478). According to the results obtained in this study we can conclude that measurements of the acute phase proteins, haptoglobin and serum amyloid A predominantly in milk, may be useful tool in diagnosing mastitis and may be a useful marker of milk quality.
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