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Somaclonal variability in callus culture of Lycopersicon hirsutum f. typicum and Lycopersicon chilense

100%

Rzepka-Plevnes D., Kulpa D., Palka E., Wisniewska M.

Acta Scientiarum Polonorum. Hortorum Cultus

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2010

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tom 09

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nr 4

The aim of the study was to induce somaclonal variability in the callus culture of L. hirsutum f. typicum and L. chilense and to characterize them in respect to tolerance to salinity. Callus was initiated on the cotyledon fragments grown on the medium supplemented with NAA and BAP. The tolerance of callus to salt was tested on MS media containing NaCl in concentration 25, 50, 75, 100, 200 mM. Callus tolerant to 100 mM NaCl was next regenerated by ten weeks on the media with different doses of growth regulators. After this time genetic differences between selected fragments and control (MS medium containing no growth regulators) were determining using ISSR-PCR method. The results show that NaCl concentration significantly affects the regeneration of callus in L. hirsutum f. typicum and L. chilense. The dose 200 mM NaCl of the medium results, in both species, in callus dying. Comparing the genetic similarity of examined callus samples with the control ones in both species, it may be stated that the differences in their response to NaCl and applied growth regulators were generally in the range 2–30%.

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Morphological character of plants and fruits quality in cucumber after recurrent regeneration in in vitro culture

100%

Gajc-Wolska J., Szwacka M., Malepszy S., Seroczynska A.

Folia Horticulturae

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2007

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tom 19

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nr 2

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Histological study on embryogenic culture of the oak [Quercus robur L.]

100%

Ostrolucka M. G., Sunderlikova V., Tsvetkov I. N.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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1999

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tom 41

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nr 1

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Hacquetia epipactis (Apiaceae) – rozmnażanie in vitro i zdolność kiełkowania nasion w warunkach ex situ

88%

Nowak B., Sitek E., Gajewski Z.

Acta Botanica Silesiaca

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2011

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tom 06

Demographic studies often point out the poor state of insular populations of Hacquetia epipactis. The species is under strict protection. This is due to both natural and anthropogenic factors, including the collection of intact plants for gardens. As a form of integrated protection, an attempt at ex situ propagation was undertaken using seeds and tissue culture.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Acta Botanica Silesiaca

1 Acta Scientiarum Polonorum. Hortorum Cultus

1 Folia Horticulturae

1 Gajc-Wolska J.

1 Gajewski Z.

1 Kulpa D.

1 Malepszy S.

1 Nowak B.

1 Ostrolucka M. G.

1 Palka E.

1 Rzepka-Plevnes D.

1 Seroczynska A.

1 Sitek E.

1 Sunderlikova V.

1 Szwacka M.

1 Tsvetkov I. N.

1 Wisniewska M.

1 2011

1 2010

1 2007

1 1999

Somaclonal variability in callus culture of Lycopersicon hirsutum f. typicum and Lycopersicon chilense

Morphological character of plants and fruits quality in cucumber after recurrent regeneration in in vitro culture

Histological study on embryogenic culture of the oak [Quercus robur L.]

Hacquetia epipactis (Apiaceae) – rozmnażanie in vitro i zdolność kiełkowania nasion w warunkach ex situ