Mannosylphosphodolichol synthase (MPD-synthase) (EC 2.4.1.830) catalyzing formation of MPD from GDPMan and dolichylphosphate (PD) has been purified from T. reesei cellular membranes almost to homogeneity. Selective solubilization of the enzyme was followed by one step purification on Phenyl-Sepharose column. SDS/ PAGE of the purified enzyme fraction revealed the presence of a protein band of 31 kDa corresponding to the apparent molecular mass of the MPD-synthase purified from S. cerevisiae [Babczinski, P. et al. (1980) Eur. J. Biochem. 105,509-515; Haselbeck A. (1989) Eur. /. Biochem. 181, 663-6681. During solubilization, the enzyme was stabilized by the presence of a lipophilic substrate dolichylphosphate and phospholipids as well as by protease inhibitors. The Phenyl-Sepharose purified enzyme had an absolute requirement for dolichylphosphate and was activated by cAMP dependent protein kinase.
Green tea offers not only pleasant, delicate flavor, but also provides health benefits. The extract contains, among others, polyphenols responsible for antioxidant and anti-inflammatory properties. They reduce the risk of cancer and their presence exerts preventive activity against cardiovascular diseases. Analysis of selected physicochemical and solubilizing properties of pharmacopoeial-true solutions of dry green tea extract. The caffeine content was determined in the extract and in dry residue after solubilization by high performance liquid chromatography. The process of micellar solubilization of cholesterol granules and ketoprofen was carried out in model solutions of green tea extract. The obtained results indicate that the prepared ‘ex tempore’ leaf green tea infusion subjected to short thermal exposure will be characterized by significant solubilization abilities. The outcomes of the research pointed to the possibility of developing a solid oral dosage form with titrated dry green tea extract of expected pharmacotherapeutic profile.
Badania prowadzono w celu ustalenia optymalnych warunków roztwarzalności fosfogipsu pochodzącego z Gdańskich Zakładów Nawozów Fosforowych w wodnych roztworach: KOH, Ca(OH)2, HNO3, kwasu cytrynowego, K2CO3, KC1 i NH4Cl. Stwierdzono, że pod wpływem wodnych roztworów KOH, Ca(OH)2, K2CO3 następuje neutralizacja fosfogipsu z jednoczesnym unieruchomieniem zawartych w nim fosforanów. W przypadku KOH i K2CO3 następuje roztwarzanie głównego składnika fosfogipsu, tj. CaSO4. K2CO3 powoduje znaczny wzrost roztwarzalności fluorków zawartych w fosfogipsie.
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