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The toxic action of cadmium in the bone tissue is known, but its mechanisms are still unexplained. We examined whether Cd influences collagen content and its solu­bility in the femoral bone of three-week-old female rats exposed to 5 or 50 mg Cd/l in drinking water. Non-cross linked collagen was extracted with 0.5 M acetic acid, and two acid-insoluble collagen fractions were extracted with pepsin and 4.0 M guanidine hydrochloride, respectively. SDS/PAGE showed the presence of two colla­gen types, I and V, in all three extracted fractions. Exposure of rats to Cd for 6 months increased the amount of acid-soluble collagens type I and V and decreased the level of acid-insoluble collagens. The amount of total collagen extracted from the bones of rats exposed to 50 mg Cd/l was reduced by about 14% as compared to con­trol and those intoxicated with 5 mg Cd/l. The solubility of type I bone collagen (de­termined as the percentage of acetic-soluble fraction of total collagen) was increased 2.9- and 3.0-fold in rats intoxicated with 5 and 50 mg Cd/l, respectively. Similarly, the solubility of type V collagen was increased 2.3- and 2.7-fold, respectively. Our re­sults indicate that Cd treatment affects bone collagen by decreasing its content and increasing its solubility.
Using the general Hildebrand-Scatchard-Fedors theory of solubility, the mole fraction (x2) of solubility of phytochemicals contained in the dry green tea leaves was calculated which determines the profile of pharmacological activity.The applicative purpose of the study was to estimate the actual solubility of phytochemicals – S|real.| [mol/dm3] in water and in water-ethanol solutions of diversified polarity (εM) for their selective extraction and optimal formulation of oral solid dosage form.The basic physico-chemical and structural quantities of phytochemicals and corresponding mathematical equations of general Hildebrand-Scatchard-Fedors theory of solubility were used to calculate the actual solubility – S|real.| and the level of hydrophilic-lipophilic balance (HLB).The calculated actual solubility values – S|real.| [mol/dm3] collated with correlation equations enabled the assessment of phytochemical capability for the process of mass exchange on phase boundary. Correlation equations for the dependence log P = f (– log S|real.|) point to the structural preferences of phytochemicals in the kinetics of the mass exchange (diffusion) through the natural phase boundary.Calculations and correlations between the values characterizing the actual solubility – S|real.|, media polarity (water, ethanol and their solutions) and the partition coefficient (log P) including the level of hydrophilic-lipophilic balance (HLB) show that basing on thermodynamic components of the general Hildebrand-Scatchard-Fedors theory of solubility, the diffusion profile of phytochemicals contained in the green tea extract (Ext. Camellia sinensis L. aqu. siccum) through the biological phase boundary as well as optimal choice of the extraction medium for selective extraction of the class of phytochemicals can be estimated.
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Extrusion-cooking of wheat starch

72%
During the study the impact of extrusion-cooking process parameters on the wheat starch physical properties changes was investigated. The process was characterized by small energy consumption within a range 270-1069 kJkg-1. Extrusion-cooking technique allows creating the degree of gelatinization of processed starch. It is possible to achieve low or high level of gelatinization depending on the process parameters. Expansion index of the extrudates decreased with increase of wheat starch moisture content. It was found that the use of the extrusion process resulted in increase of water absorption and cold water solubility of starch. The highest value of WAI was 690% and WSI was 19%.
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Aluminium solubility in the presence of citric acid

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The present study points to an important role of citric acid in limiting toxicity of aluminium ions for pea plants which was confirmed by the plants’ growth, development and yield. Excess of aluminium influenced plant nutrition with Ca, P, Mg, and K as this toxic element did not only gather in the roots of the studied plants but also in their above-ground parts. Limitation of aluminium toxicity by its complexing with citric acid clearly influenced a increase in the Fe uptake by plants and an decrease in the Mn uptake which was statistically proved.
The quality of five commercial monocalcium phosphates (MCP) and dicalcium phosphates with natural admixtures of hydrated and dehydrated forms was assessed on the basis of their crystalline phases determined by roentgenographic irradiation, Ca and P contents, and solubility in water, 2% citric acid, 0.4% HCl, and ammonium citrate solutions. The phosphates were used in diets fed between days 1 and 35 of life to 300 Hubbard Flex male broilers, 6 replications (cages) of 10 chickens per treatment. Performance indices, Ca, P and Mg retention, and the concentration of these elements in blood and bone ash, as well as the physical parameters of femur and tibia bones were measured. The content of P in phosphates varied between 17.7% and 23%, their solubility in citric acid and HCl solutions ranged between 89–99%. The roentgenograms indicated that phosphate No. 1 contained pure MCP; No. 2, MCP with admixture of anhydrous dicalcium phosphate (DCP); No. 3, DCP with an admixture of MCP; No. 4, dicalcium phosphate dehydrate (DDCP); No. 5, DDCP with an admixture of DCP. The type of phosphate used in chicken diets did not influence body weight or feed intake. Phosphorous retention, Ca and P in serum, and some bone parameters were better in chickens fed the diet containing pure MCP (P<0.01). Lower concentrations of Ca and P in bones and worse parameters of bone elasticity were found in chickens fed diets containing DDCP with DCP. In the biological experiment, the overall best results were obtained in chickens fed diets containing pure hydrated monocalcium phosphate
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Modyfikacja skrobi ziemniaczanej metodą ekstruzji

72%
W trakcie badań określano wpływ parametrów procesu ekstruzji na zmiany właściwości fizycznych skrobi ziemniaczanej. Proces charakteryzował się niedużą energochłonnością. Współczynnik ekspandowania ekstrudatów malał wraz ze wzrostem wilgotności surowca. Stwierdzono, że zastosowanie procesu ekstruzji powodowało wzrost wodochłonności i rozpuszczalności skrobi. Najwyższa wartość współczynnika wodochłonności (WAI) wynosiła 569%, a współczynnika rozpuszczalności w wodzie (WSI) 43%.
The effect of three different moisture statuses, i.e., 150, 200 and 300% FWC (Field Water Capacity) on copper (Cu), iron (Fe) and manganese (Mn) solubility and solution activity was investigated on soil samples characterised by different levels of copper contamination. Soils (200 g) were weighted into polyethylene containers (500 cm3) and amounts of bidistilled water were properly added to reach the targeted moisture status of 150, 200 and 300% of the FWC. The incubation was held under laboratory conditions at the temperature of 19-20oC for a period of 30 days. Supernatants (10 cm3) were collected at given intervals of time, filtered and analysed for pH (potentiometrically) as well as for Cu, Fe, and Mn (spectrophotometrically). It was found that the activity of Cu2+ ions decreased gradually with increasing pH of the solution, irrespective of the moisture status and that this process was more pronounced for 150 and 200% than for 300% FWC. The activity of Fe varied in a narrow range i.e. –5.0 and –6.0 molcdm-3 and was related to pH changes (in the range 4.0-7.5). The impact of increased moisture status on the solubility process was less pronounced. Further studies should be undertaken in order to elucidate such Fe behaviour. Manganese solution activity varied mostly between – 4.0 and –7.0 molcdm-3, and was found to be less sensitive to pH changes. But it must be pointed out that the effect of high pH on the increase of Mn activity was limited, which implied that Mn2+ activity was moisture-dependent, basically. Care should be taken to avoid any submersion of soils subjected to contamination or pollution by trace metals, since any excess of stagnant water (anoxic conditions) leads to increased solubility and simultaneous activity of trace metals in the solution. This process is greatly strengthened by significant amounts of soil-born Fe and organic matter.
This paper presents the research on permeation enhancing properties of Nepeta cataria var. citriodora (catnip) dry extract in comparison to oleanolic acid and ursolic acid. Progesterone was chosen as a model substance for permeation test. The hydrogels made of hydroxypropylmethylcellulose with progesterone, enhancers and ethanol were applied in the study. The in vitro progesterone penetration test was based on the method proposed by Fürst, using artificial lipophilic membranes which were made of colloxylin and dodecanol. Statistical analyses showed an increase in penetration of progesterone caused by catnip dry extract in comparison to ursolic acid and blank sample. HPLC assay was applied to study the effect of enhancers on progesterone physicochemical properties. The solubility of progesterone was tested in solvent systems corresponding to liquid phases of gels. The statistical increase in progesterone solubility was observed in the presence of dry extract in comparison to the result from ursolic acid-containing sample. The partition coefficient of progesterone was evaluated by standard procedures. The statistically significant reduction of log P values for progesterone was determined in the presence of catnip dry extract.
The chemical composition and solubility of acid whey powders obtained from nanofltered and neutralised wheys were studied. The change in the composition and acidity of acid whey powders caused by nanofiltration and neutralisation significantly influenced their solubility. With a decrease in ash content and acidity, the solubility was observed to increase. The correlation was found between ash content (r=0.843) and acidity to insolubility index (r=0.709). The study showed that the location of neutralisation in the technological process of acid whey powder had a significant influence on its properties. More favourable would be to apply acid whey neutralisation after nanofiltration. Diafiltered acid whey powders were characterised by the best properties, even better than those of the control sweet whey powder.
W preparatach otrzymanych na skalę pilotującą w Japonii z lucerny, owsa, trawy rajgras suszonych liofilizacyjnie i rozpyłowo a także w koncentratach z lucerny wzbogaconych w naturalne pigmenty oznaczono zawartość białka i jego rozpuszczalność oraz poziom związków fenolowych.
W pracy przedstawiono wyniki badań nad właściwościami trójskładnikowych układów, kwercetyna/-cyklodektryna/woda. Stwierdzono zwiększenie rozpuszczalności kwercetyny w wodzie na drodze kompleksowania tego związku przez cyklodesktrynę. Określono zdolność zmiatania wolnych rodników przez otrzymane układy oraz podstawowe parametry opisujące proces kompleksowania. Po raz pierwszy zastosowano modele Apelblata i Buchowskiego-Ksiazczaka do modelowania procesu zwiększenia rozpuszczalności kwercetyny na drodze kompleksowania. W pracy przeprowadzono także badania nad dynamiką procesu kompleksowania. Stwierdzono, że kwercetyna tworzy z -cyklodektryną kompleksy o stechiometrii 1:1. Powstanie kompleksu zwiększa rozpuszczalność kwercetyny w wodzie, jednak nie wiąże się to bezpośrednio ze wzrostem właściwości antyutleniających otrzymanych roztworów. Fakt ten jest najprawdopodobniej konsekwencją ograniczenia dostępności niektórych grup funkcyjnych kwercetyny na drodze maskowania wewnątrz cząsteczki cyklodekstryny.
The results indicated that corn had a higher resistance to swelling than rice varieties but a higher solubility than rice varieties with the same amylose content. The gelatinization enthalpy was also higher. The higher the amylose content was, the lower was the fall in the amylographic consistency during the cooking period (observed with rice samples). The consistency at the end of the cooking step and rétrogradation was directly related to the amylose content. All samples were significantly affected by the extrusion variables (screw speed and die diameter) particularly at 15% moisture content. The degree of cooking increased as the die diameter and amylose content decreased. The effect of screw speed (rpm) was dependent on the moisture level, being related directly at 15% and inversely at 30%. The thermograms of extruded samples indicated that at the extrusion temperature of 150°C a complete melting of crystallites was not achieved, transitions were shifted to higher temperatures and the lipid complex formation was observed during extrusion, which seemed to be higher for extruded samples with 15% moisture.
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