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Soil organic matter (SOM) is an essential soil constituent. The deficiency of SOM is an essential problem in many regions in Poland. Diversity of organic matter and the environment create varying structures and compositions, and thus differences in properties of humic substances (HS) especially humic acids (HAs). It was found that soil amendment with organic matter resulted in improving of soil and HAs properties compared with non-treatment ones. The results obtained provided the following evidences. With respect to HAs: an increasing content of carboxylic groups in HAs from amended soils; a major content of aromatic ring systems; higher carbon, nitrogen, hydrogen, and sulphur and lower oxygen contents comparing to the control; the addition of straw caused an increase of carbon content in HAs particles and, consequently a decrease of the C:H ratio; higher content of oxygen functional groups compared with non-treated ones. Organic matter from straw is more resistant to fast decomposition as compared to compost and it shows higher long-term sorption capacity. Therefore, straw may be an equivalent to natural sources of SOM in terms of agriculture and ecosystems protection.
The aim of the study has been to define the stocks and the basic properties of soil organic matter in agricultural-used soil sequence located in the former shoreline of disappearing lakes. The study area is located in the catchment of the Sumowskie Lakes, the Brodnica Lake District, North-Eastern Poland. The investigations involved preparation of five soil pits located in the south-western part of the former Sumowskie Lake bottom and on the southern slope of the adjacent kame hill. The greatest soil transformation is reflected in the quality and quantity of the soil organic matter. Indexes of organic matter quality and TOC stocks are significantly changing along the studied transect. The strongly humidified organic matter is found in mursh horizons. Gyttja layers above the groundwater level have a medium humification index. Horizons saturated with water are very low humidified. TOC stocks drop along the analysed soil sequence from the biogenic plain to the top of the kame hill.
Dehydrogenases are exclusively intracellular enzymes, which play an important role in the initial stages of oxidation of soil organic matter. One of the most frequently used methods to estimate dehydrogenase activity in soil is based on the use of triphenyltetrazolium chloride as an artificial electron acceptor. The purpose of this study was to compare the activity of dehydrogenases of forest soils with varied physicochemical properties using different triphenyltetrazolium chloride assays. The determination was carried out using the original procedure by Casida et al., a modification of the procedure which involves the use of Ca(OH)2 instead of CaCO3, the Thalmann method, and the assay by Casida et al. without addition of buffer or any salt. Soil dehydrogenase activity depended on the assay used. Dehydrogenase determined by the Casida et al. method without addition of buffer or any salt correlated with the pH values of soils. The autoclaved strongly acidic samples of control soils showed high concentrations of triphenylformazan, probably due to chemical reduction of triphenyltetrazolium chloride. There is, therefore, a need for a sterilization method other than autoclaving, ie a process that results in significant changes in soil properties, thus helping to increase the chemical reduction of triphenyltetrazolium chloride.
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Sustainable development of the farms in Poland

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Based on statistical data of the Central Statistical Offi ce regarding Polish farms two linear-dynamic multicriteria optimization models have been created. The first model concerned plant production, the other plant and animal production. In both models, objective functions maximized agricultural income and production, and minimized loss of organic soil matter. Balancing these three objectives is the essence of a farm’s sustainable development. The models have been solved with goal programming. The optimal solution yielded a production structure allowing for the highest quality of production, under given conditions of agricultural income, with no degradation of the natural environment. The goal of the following article is to confirm whether it is possible to simultaneously realize the production, economic and ecological goals of Polish farms over the course of four years.
Due to the ease and low cost of implementation, a commonly used method of determining the humus content in soils is the loss-on-ignition (LOI) method. Several regression equations and transformation factors are reported for LOI conversion to soil organic matter (SOM) or total organic carbon (TOC) content. The vast majority of the conversion factors have been developed for surface horizons of lowland soils, while there are only few findings from the mountainous areas. 476 mineral and 79 organic (forest litter) samples from 31 soil profiles located in different altitude zones of the Karkonosze Mountains and under different vegetation were used for analysis. LOI was determined by the drying-weighing method and TOC by the dry combustion method with CO₂ absorption. The average LOI/TOC ratio was about 2, but varied in accordance to LOI value. At the LOI value above 8–10%, the LOI/TOC is maintained at a constant level of 2.0, but with a decreasing LOI it may significantly extend, up to 20 at LOI <1%. In the mountain soils, the best compatibility of TOC determined and estimated based on LOI was obtained when using different conversion factors ( 7.3, 5.0, 3.1 and 2.0) for the four LOI ranges (0–2, 2–4, 4–8, and > 8%, respectively) or when using separate regression equations for LOI of <8 and >8%. Because of huge TOC overestimation by LOI method at LOI values lower than 8–10%, the conversion LOI to TOC is not recommended, unless the direct measurement of TOC content is currently unavailable (e.g. in archival databases).1
Three sites with both degraded alpine Kobresia meadow (DM) and adjacent mix-seeded perennial grasses pastures (MSP) in the headwater region of the Yellow River, Qinghai-Tibetan Plateau, were selected to investigate plant and soil organic carbon (SOC), total nitrogen (TN) and their isotope composition. The SOC and TN in the top soil layer to a depth of 10 cm were significantly affected by ploughing and planting mix-seeded perennial grasses. The SOC content in 0–10 cm soil layer of MSPs was 25.6%, 5.5% and 12.9% lower than those of DMs at the I-III sites, respectively, and the rate of OC density loss was 23.8, 14.5 and 18.2%, respectively. The soil TN content in 0–10 cm soil layer of MSPs was 16.6%, 2.2% and 9.4% lower than those of the DMs at three sites, respectively, and the TN density was 15.6, 10.6 and 15.3% lower than those of DMs, respectively. The plant and soil ¹³C values (-27.03‰, -25.16‰, respectively) suggest that the vegetation of both DMs and MSPs are C₃ plant communities. The 15 N value in the soil (>4‰) was significantly greater than in plants (<2‰). No differences of either ¹³C or ¹⁵N abundance between MSPs and DMs at Site I and II, but were found at Site III, indicating that the effects were site specific. The rehabilitation of a degraded Kobresia meadow has a significant influence on the soil properties, SOC and TN. Caution should be taken in site selection before performing conversion.
The aim of this work was to test the suitability of microbiological methods for the ecotoxicological evaluation on the example of soils of two sites (I and II) (more and less contaminated and situated in Saxony-Anhalt, Germany). Contents of Zn, Pb, Cd, Ni, Cr, and Cu in soil ranged 787–210, 210–110, 8–1, 49–12, 130–17, and 161–70 mg kg⁻¹, respectively being significantly higher in site I. The sites differ also in respect to pH (5.2–7.5), Corg (5.66–8.27%) and type of soil. The parameters tested were following: substrate-induced respiration (SIR), activity of luminous bacteria, substrate utilization patterns on BIOLOG ECO-and GN-plates, and phospholipid fatty acid (PLFA) profiles. The respiratory coefficient QR, peak respiratory maximum and BIOLOG ECO-plates were suitable for an ecotoxicological assessment of contaminated soils. QR values (> 0.3) and peak maximum values (> 40 h) indicated stress of soil microorganisms at the most heavy metal contaminated site (site I). PLFA analysis can be used to detect various environmental stresses in the soil. The trans/cis ratio of monosaturated fatty acids (> 0.1) and the fungal/bacterial biomass ratio were able to distinguish the stress conditions in soils connected with heavy metal contamination. With increasing heavy metal content in soils the PLFA pattern are changed directionally.
No consensus has emerged on the sensitivity of soil respiration to increasing temperatures under global warming due partly to the lack of data and unclear feedbacks. Our objective was to investigate the general trends of warming effects on soil respiration. This study used meta-analysis as a means to synthesize data from eight sites with a total of 140 measurements taken from published studies. The results presented here suggest that average soil respiration in forest ecosystems was increased approximately by 22.5% with escalating soil temperatures while soil moisture was decreased by 16.5%. The decline in soil moisture seemed to be offset by the positive effects of increasing temperatures on soil respiration. Therefore, global warming will tend to increase the release of carbon normally stored within forest soils into the atmosphere due to increased respiration.
The effect of aluminum complexation on the net decomposition rate of purified humic acid (HA) and fulvic acid (FA), extracted from a podzol B horizon in the Netherlands, were studied at room temperature (20°C). Net decomposition rates were determined from C02 evolution from samples as measured by gas-chromatography. Increasing aluminum additions significantly reduced the decomposition rate of HA, of FA ,and of organic matter in podzol B horizons, whereas pH had no notable effect on decompostion rates. Results indicate that at the current high depletion rates of organically-bound soil Al, due to acid rain, the decomposition of soil humic compounds may increase significantly. This may have serious consequences for the ecologically relevant pool of humic compounds in forest soils.
Abundance of microorganisms and their communities’ structure in disturbed peatlands characterized by different chemical properties and plants cover were studied. Naturally regenerating four disturbed peatlands from different regions of Lithuania were selected for the investigation. The aim of the study was to investigate invasive moss Campylopus introflexus cover effect on microbial communities. The peat under Eriophorum angustifolium, Polytrichum strictum and bare peat were sampled for the comparison. The abundance of microorganisms in sampling plots depends on the content of total nitrogen, potassium, soil organic matter and pH as well as on growing plants. In the peat under C. introflexus, the number of ammonifying and mineral N assimilating bacteria was significantly lower in comparison with other plants or bare peat. The results on fungi abundance in peat under C. introflexus allow to predict that the distribution of moss doesn‘t affect negatively the development of fungi. A total of 236 fungal strains belonging to 21 genera were isolated from the studied peat samples. Species from the Penicillium, Trichoderma and Mortierella genera dominated among them. The structure of microbial communities varied in separate peatlands and under various plants. However, all examined microorganism communities seemed to be able adapted to organic matter degradation.
The objective of the study was the estimation of quality of organic matter of the humus horizon of soil fertilised every year, for ten years, with spent mushroom (Agaricus L.) substrate, as compared to a soil without such amendment. Fractionation of organic matter was performed, as well as quality analyses of humic acids and fractionation of organic nitrogen complexes. In the humus horizon of the soil fertilised with spent mushroom substrate lower acidity was noted, and higher levels of C, N, P and Ca. In the soil amended with the substrate greater accumulation of soil humus was noted (by nearly 50%), and especially of the fraction of humic acids, which had a positive impact on increasing the value of the ratio of humic to fulvic acids. The fertilisation with the substrate only slightly modified the quality parameters of humic acids. In the soil fertilised with the substrate a higher level of carbon and nitrogen was observed – related with the soluble forms of organic matter, as well as greater accumulation of nitrogen, mainly in a stable non-hydrolysing form.
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