Wyniki wyszukiwania

1

Nitric oxide as a signalling molecule

100%

Wrzosek A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

AMP-deaminase from hen stomach smooth muscle - physico-chemical properties of the enzyme

88%

Swieca A., Rybakowska I., Koryziak A., Klimek J., Kaletha K.

Acta Biochimica Polonica

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2004

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tom 51

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nr 1

AMP-deaminase from hen stomach smooth muscle was isolated and physico-chemical properties of the purified enzyme were investigated. The enzyme had an activity optimum at pH 6.5, and poorly deaminated the substrate analogues tested. At optimum pH (6.5), in the absence of regulatory ligands (control conditions), the enzyme manifested hyperbolic substrate-saturation kinetics with half-saturation constant GS0.5) of about 4.5 mM. Additions of adenine nucleotide effectors (ATP, ADP) activated the enzyme strongly at all the concentrations tested, diminishing significantly the value of S0.5 constant. In contrast, the regulatory effect of orthophosphate was variable, and depended on the orthophosphate concentration used. The molecular mass of the enzyme subunit determined in SDS/PAG electrophoresis was about of 37 kDa. The obtained results suggest that in different types of hen muscle, similarly as in humans and rats, expression of AMP-deaminase is under the control of independent genes.

3

Role of immunohistochemical and histochemical profiling in H and E-based diagnosis of scrotal leiomyosarcoma of dartos muscle

75%

Wincewicz A., Lewitowicz P., Sulkowska U., Sulkowski S., Horecka-Lewitowicz A., Koziel D., Gluszek S.

Folia Histochemica et Cytobiologica

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2013

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tom 51

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nr 4

4

Synthesis and biological properties of new chimeric galanin analogue GAL(1-13)-[Ala10,11]ET-1(6-21)-NH2

75%

Ruczynski J., Konstanski Z., Cybal M., Petrusewicz J., Wojcikowski C., Rekowski P., Kaminska B.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 2

Several chimeric peptides consisting of the N-terminal fragment of galanin (GAL) and C-terminal fragments of other bioactive peptides (e.g. substance P, bradykinin, neuropeptide Y, mastoparan) have been synthesized and reported as high-affinity galanin receptor antagonists. Recently we have synthesized a new chimeric peptide, GAL(1-13)-[Ala10,11]ET-1(6-21)-NH2, consisting of the N-terminal fragment of GAL and the C-terminal fragment of endothelin-1 (ET-1) analogue. This chimera was previously shown to be a moderate-affinity ligand to hypothalamic galanin receptors with a KD value of 205 nM. However, its biological action has been unknown so far. In our studies we characterized the biological properties of this new chimeric analogue, investigating its action on rat isolated gastric smooth muscles and influence on insulin secretion from rat isolated islets of Langerhans. Data acquired in the course of our studies suggest that analogue GAL(1-13)-[Ala10,11]ET-1(6-21)-NH2 does not seem to be a potent galanin receptor antagonist in the gastrointestinal tract.

5

Motifs of the caldesmon family

75%

Czurylo E. A.

Acta Biochimica Polonica

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2000

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tom 47

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nr 4

Seven highly conserved regions were found in caldesmon molecules from various sources using the multiple sequence alignment method. Their localization coincides with regions where the binding sites to other proteins were postulated. Less conserved and highly divergent regions of the sequences are described as well. These results could refine the planning of caldesmon gene manipulations and accelerate the precise localization of binding sites in the caldesmon molecule and, as a consequence, this could help to elucidate its function in smooth muscle contraction.

6

Gap junction density in human myometrium at term revealed by an anti-peptide antibody and laser scanning confocal microscopy

75%

Kilarski W. M., Severs N. J., Gourdie R. G., Rezapour M., Backstrom T., Roomans G. M., Ulmsten U.

Folia Histochemica et Cytobiologica

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1993

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tom 31

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nr 4

7

The role of tyrosine kinase in prostaglandin E2 and vanadate-evoked contractions in rabbit duodenum in vitro

75%

Grasa L., Arruebo M. P., Plaza M. A., Murillo M. D.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

Prostaglandin E2 (PGE2) can interact with at least four cell surface receptors (EP1-EP4) in smooth muscle, which evokes a variety of intracellular responses depending on the G protein to which the cell surface receptors are coupled. The activation of G protein-coupled receptors and receptor tyrosine kinases can lead to the phosphorylation of tyrosine residues of various cellular proteins. The aim of this study was to examine the role of tyrosine phosphorylation in PGE2, vanadate and carbachol-evoked contractions. PGE2, vanadate, and carbachol induced contractile motor responses in the longitudinal smooth muscle of rabbit duodenum. PGE2-evoked contractions decreased in the presence of genistein or tyrphostin B44. PGE2-evoked contractions increased in the presence of vanadate. Vanadate-evoked contractions decreased in the presence of genistein. In contrast, tyrphostin 47 increased the vanadate-evoked contractions. Vanadate-evoked contractions were reduced in the presence of Ca2+-free solutions, verapamil, or indomethacin. U-73122 decreased PGE2-evoked contractions. Carbachol-evoked contractions decreased in the presence of genistein, tyrphostin B44 or tyrphostin 47. Our results suggest that PGE2, vanadate or carbachol-evoked contractions are mediated by protein tyrosine phosphorylation. Protein tyrosine phosphorylation might cause an increase in calcium influx through voltage-dependent channels and the release of prostaglandins in the longitudinal smooth muscle of the rabbit duodenum.

8

Insight into the kinetics and the mode of the interaction between smooth muscle calponin and F-actin

75%

Kolakowski J., Dabrowska R.

Acta Biochimica Polonica

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2002

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tom 49

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nr 2

Kinetics of the smooth muscle calponin-F-actin interaction was studied by stopped- flow measurements of light scattering and fluorescence intensity of pyrene-labelled F-actin. The intensity and character of the changes in light scattering, and thus the mode of calponin binding to actin filaments leading to changes in their shape and bundling, depend on the molar ratio of the two proteins. Parallel measurements of pyrene-fluorescence quenching upon calponin binding revealed that intrinsic conformational changes in actin filaments are delayed relative to the binding process and are not markedly influenced by the mode of calponin binding. Bundling of actin filaments by calponin was not correlated with fluorescence changes and thus with alterations in the structure of actin filaments.

9

Effects of protoporphyrins on production of nitric oxide and expression of vascular endothelial growth factor in vascular smooth muscle cells and macrophages

75%

Jozkowicz A., Dulak J.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 1

Heme oxygenase-1 (HO-1), an inducible enzyme degrading heme to biliverdin, iron and carbon monoxide, is involved in regulation of inflammation and angiogenesis. Tin protoporphyrin (SnPPIX) and zinc protoporphyrin (ZnPPIX) are commonly used as competitive inhibitors of HO-1. We aimed to compare the effects of SnPPIX and ZnPPIX on the production of vascular endothelial growth factor (VEGF), activity of inducible nitric oxide synthase (iNOS) and cell viability. All experiments were performed on rat vascular smooth muscle cells and murine RAW264.7 macrophages treated with 3-10 ,uM protoporphyrins. Some cells were additionally stimulated with IL-1β or with lipopolysaccharide. After a 24 h incubation period SnPPIX and ZnPPIX significantly reduced the generation of VEGF in vascular smooth muscle cells and RAW264.7, both in resting and stimulated cells. The inhibitory potentials of both protoporphyrins on VEGF synthesis were very similar. In contrast, analysis of iNOS activity revealed that results obtained with different HO-1 inhibitors are discrepant.

10

The use of magnesium in bronchial asthma: a new approach to an old problem

75%

Kowal A., Panaszek B., Barg W., Obojski A.

Archivum Immunologiae et Therapiae Experimentalis

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2007

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tom 55

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nr 1

11

Size, shape and secondary structure of calponin

75%

Czurylo E. A., Eimer W., Kulikova N., Hellweg T.

Acta Biochimica Polonica

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2000

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tom 47

|

nr 3

The overall size and shape of the chicken gizzard calponin (CaP) h1 molecule was investigated by dynamic light scattering (DLS) measurements. From the DLS experiments, a z-averaged translational diffusion coefficient is derived (5.75 0.3) 10-7cm2s-1, which corresponds to a hydrodynamic radius of 3.72 nm for calponin. The frictional ratio (1.8 for the unhydrated molecule and 1.5 for the hydrated one) suggests a pronounced anisotropic structure for the molecule. An ellipsoidal model in length 19.4 nm and with a diameter of 2.6 nm used for hydrodynamic calculations was found to reproduce the DLS experimental data. The evaluation of the secondary structure of CaP h1 from the CD spectra by two independent methods has revealed that it contains, on average, 23% helix, 19% beta-strand, 18% beta-turns and loops, and 40% of remainder structures. These values are in good agreement with those predicted from the amino-acid sequence. Predictions used for CaP h1 were applied to other isoforms of known sequences and revealed that all calponins share a common secondary structure. Moreover, the predicted structure of the calponin CH domain is identical to that found by X-ray studies of the spectrin, fimbrin and utrophin CH domains.

12

The effect of a selective inhibition of potassium channels on the relaxation induced by nitric oxide in the human pregnant myometrium

75%

Modzelewska B., Kleszczewski T., Kostrzewska A.

Cellular and Molecular Biology Letters

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2003

|

tom 08

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nr 1

The aim of this study was to investigate whether apamin-sensitive K+ channels play a role in the NO induced relaxation of the human pregnant myometrium. Concentration-response curves for sodium nitroprusside (SNP) (10-9 – 10-4 M) were constructed in the absence and presence of 10-8 M apamin and 10-7 M charybdotoxin (CTX). Preincubation with apamin resulted in a significant attenuation of the relaxation caused by SNP, while pre-treatment with CTX insignificantly decreased the SNP induced relaxation. Our findings suggest that apamin-sensitive K+ channels exist in the human pregnant myometrium and play a role in modulation of the myometrium response to NO donors.

13

Adenoviral expression of a C-terminal targeting region inhibits CADTK activation in vascular smooth muscle cells

75%

He Y., Kozlowski P., Li X., Mercatante D., Earp H. S., Graves L. M.

Cellular and Molecular Biology Letters

|

1998

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tom 03

|

nr 3

14

Energy - dependent transport of Ca2 in the intracellular structures of the smooth muscle

75%

Babich L. G., Shlykov S. G., Borysova L. A., Kosterin S. A.

Biophysics of Membrane Transport

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1994

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tom 12

|

nr 2

15

The influence of beta-adrenergic receptors on uterus myoelectric activity in sheep sensitised with stilboestrol

75%

Dejneka J., Zieba D., Rauluszkiewicz S.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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1998

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tom 01

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nr 1

The research was conducted on 5 inter-breed hybrid sheep, at the age of 10 to 24 months and of the body mass of 30-45 kg, which had bipolar electrodes implanted to uterus horns and shank. The registration of uterus myoelectric activity was performed on sheep 24 and 48 hours after sensitising with Stilboestrol, by the use of Reega Duplex TR XVI electroencephalograph. After recording the output activity, the sheep were applied intravenously in continuous injection: adrenaline 0,12-0,18 μg ˇ kg-1 ˇ min-1, oxytocin 0,25-0,4 i.u., propranolol 1mg ˇ kg-1, isoprenaline 0,4-1,5 μg ˇ kg-1 ˇ min-1, salbutamole 0,4-0,8 μg ˇ kg-1 ˇ min-1. The experiment results are presented in the inclosed drawings. The conducted research revealed that the stimulation of beta-adrenergic receptors causes the decrease or inhibition of uterus myoelectric activity of sheep sensitised with Stilboestrol.

16

Flavonoids and nitric oxide synthase

75%

Olszanecki R., Gebska A., Kozlovski V. I., Gryglewski R. J.

Journal of Physiology and Pharmacology

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2002

|

tom 53

|

nr 4,1

Induction of NOS-2 in macrophages and smooth muscles within vascular wall with concomittant suppression of endothelial NOS-3 activity is considered to be a hallmark of vascular inflammation that triggers atherogenesis. Accordingly, drugs designed to reverse these changes should not only support vaning function of NOS-3 but also suppress proinflammatory NO production by NOS-2. It means that using selective inhibitors of induction of NOS-2 (they spare ex definitione constitutive activity of NOS-3) is a more rational approach than using isselectivel. inhibitors of activity of previously induced NOS-2. First of all, those drugs are never sufficiently selective. In our work we tried to identify inhibitors of NOS-2 induction within the group of flavonoids, known stimulators of NOS-3 with putative antiatherogenic effects. Representatives of four main groups of flavonoids: flavonols (kaempferol, quercetin, rutin), flavones (apigenin, primuletin), flavanols (catechine) and flavanones (hesperetin, hesperidin, naringenin) were tried on NOS-2 induction and activity in the in vitro model of LPS-treated macrophages (cell line J774.2). While none of these compounds inhibited activity of NOS-2, all with unexpectedly scattered potencies inhibited induction of NOS-2 protein in LPS-treated J774.2 cells, as evidenced by Western blotting technique. Subsequently, RT-PCR and Northern blotting methods revealed that so far the most potent compounds, kaempferol and apigenin, at micromolar concentrations did inhibit NOS-2 induction at the level of NOS-2 gene transcription. We conclude that some of flavonoids are potent inhibitors of NOS-2 induction. At the same time they may increase endothelial NOS-3 activity. Could these flavonoids become natural parents of future drugs, which will be used for reversal of inflammatory component of atherothrombosis?

17

Comparison of PTHrP expression in the epididymis of juvenile and adult European bisons

75%

Czykier E., Zabel M., Surdyk-Zasada J.

Folia Histochemica et Cytobiologica

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2003

|

tom 41

|

nr 3

18

Melatonin and serotonin effects on gastrointestinal motility

63%

Thor P. J., Krolczyk G., Gil K., Zurowski D., Nowak L.

Journal of Physiology and Pharmacology. Supplement

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2007

|

tom 58

|

nr 6

19

The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus

63%

Rajkovic J., Peric M., Stanisic J., Novakovic R., Djokic V., Rakocevic J., Tepavcevic S., Lubudovic-Borovic M., Gostimirovic M., Heinle H., Gojkovic-Bukarica L.

Journal of Physiology and Pharmacology

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2020

|

tom 71

|

nr 1

20

Comparison of the effect of alpha1- and alpha2-adrenoceptor agonists and antagonists on muscle contractility of the rabbit abdominal aorta in vitro

63%

Gnus J., Rusiecka A., Czerski A., Zawadzki W., Witkiewicz W., Hauzer W.

Folia Biologica

|

2013

|

tom 61

|

nr 1-2

Ograniczanie wyników

Nitric oxide as a signalling molecule

AMP-deaminase from hen stomach smooth muscle - physico-chemical properties of the enzyme

Role of immunohistochemical and histochemical profiling in H and E-based diagnosis of scrotal leiomyosarcoma of dartos muscle

Synthesis and biological properties of new chimeric galanin analogue GAL(1-13)-[Ala10,11]ET-1(6-21)-NH2

Motifs of the caldesmon family

Gap junction density in human myometrium at term revealed by an anti-peptide antibody and laser scanning confocal microscopy

The role of tyrosine kinase in prostaglandin E2 and vanadate-evoked contractions in rabbit duodenum in vitro

Insight into the kinetics and the mode of the interaction between smooth muscle calponin and F-actin