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The importance of mastitis in small ruminants is important from the point of view of 3 perspectives: economic (mortality of animals, treatment costs, reduced quantity and quality of milk); hygienic (the risk of infection or poisoning of consumers by consuming infected milk), and legal (definitions of bacteriological milk quality). Coagulase-negative staphylococci are the most prevalent pathogens of the mammary gland in sheep and goats with subclinical mastitis (affecting from 45 to 48% in sheep and from 60 to 80.7% in goats). Prevalence of clinical mastitis in small ruminants is usually below 5%. Several pathogens can cause mastitis, but Staphylococcus spp. are the most frequently diagnosed causal microorganisms of intramammary infections in goats and sheep. Somatic cell counts in milk of dairy ewes can be used to define subclinical mastitis and a threshold of about 200,000 to 400,000 cells/ml will accurately identify most infected ewes. In ewe milk somatic cell counts between 300,000 cells/ml and 1,000,000 cells/ml cause changes in the composition and plasmin activity, and suggest that milk secretion is in a period of transition from normal to mastitic milk. In goats infected glands also lead to an increase in somatic cell counts; however, increased somatic cell counts in milk are also caused by other non-infection factors, such as estrus, season of milking, milk yield or stage of lactation. A standard tool in the diagnosis of mastitis for small ruminants is bacteriological examination of milk. During milking of small ruminants there is usually a transmission of Gram-positive bacteria, mainly Staphylococci. Therefore, the control of mastitis in small ruminants should take into account the optimal milking routine and milking hygiene. In particularly justified cases and the large prevalence of disease antibiotic treatment should be administered in the dry-period.
Investigation into herd-level seroprevalence of caprine herpesvirus type 1 (CpHV-1) and bluetongue virus (BTV) was conducted in 2007 in Poland. It involved the entire population of goats covered by a milk recording program in 2007, which included 49 goat herds. The number of goats examined in each herd was determined statistically in order to detect the presence of at least one seropositive animal in a herd with a 95% probability and simple random method of sampling was applied. No antibodies to CpHV-1 or BTV were detected. Further calculations were carried out to determine the herd-level true seroprevalence, taking into account sensitivity and specificity of the test as well as several other factors. It can be concluded that till the middle of 2007 population of Polish goats covered by the milk recording program remained negative with respect to CpHV-1 and BTV.
Peste des petits ruminant (PPR) is a highly contagious and economically important disease of small ruminants. Plasma sialic acid is a marker of the acute phase response in pathological conditions and significant alterations within total sialic acid serum (TSA) concentrations have been documented in various diseases. The purpose of the study was to investigate changes in TSA serum concentrations in sheep suffering from peste des petits ruminants. Eighty sheep sera were tested for antibodies against PPRV with c-ELISA and 22 of the 80 sera (27.5%) were found to be positive. Twenty two sheep naturally infected by PPRV aged between 1-2 years old were categorized as the infected group and 16 clinically healthy sheep of the same age constituted the control group. Venous blood was sampled from the sheep's jugular veins. The concentration of TSA serum was measured by Warren's thiobarbituric acid assay. Clinical, hematological and biochemical changes, including liver function tests, were also evaluated. Higher concentrations of TSA serum were found in the infected sheep (84.7±13.1 mg/dL) compared to the healthy sheep (63.5±4.7 mg/dL), (P£0.001). In addition, significant correlations were determined between TSA and clinical symptoms, hematological changes and liver function tests of the infected sheep. The findings of the study indicate that TSA plays a part in the disease processes of PPR and that determining TSA serum concentrations may be used as a supplementary laboratory test in conjunction with clinical and laboratory findings when evaluating the prognosis of PPR.
A fragment of 394 bp spanning the V1-V2 variable region of small ruminant lentiviruses (SRLV) env gene was amplified by nested-PCR from peripheral blood leukocytes of 27 sheep and seven goats from five Polish flocks. PCR products were then subjected to analysis by the heteroduplex mobility assay. SRLV found in six sheep were more similar to caprine arthritis-encephalitis virus, but no viruses closely related to maedi visna virus were found in the infected goats. However, in one sheep dual infection with SRLV belonging to both A and B group was found. The results showed that interspecies infections of SRLV occur in animals living under natural conditions.
Detection of small ruminant lentiviruses (SRLVs) in sheep and goats usually relies on serological testing. In this study, we evaluated semi-nested PCR and nested PCR techniques applied as a diagnostic tool for detection of maedi-visna virus (MVV) and caprine arthritis-encephalitis virus (CAEV) in naturally infected sheep and goats, respectively. The examination of 193 ovine and 85 caprine serum samples by the ELISA revealed the presence of specific antibodies in 133 (69%) and 18 (21.2%) animals, respectively. Presence of proviral DNA was manifested in 103 (53.4%) sheep and 12 (14.2%) goats. Despite the relatively lower sensitivity of PCR, the fact of detection of proviral DNA in 19 out of 60 ovine samples and 7 out of 67 caprine samples collected from animals previously negative by ELISA was noteworthy. In conclusion, the data demonstrated that combinations of both ELISA and PCR might afford optimal detection of SRLVs infection.
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