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The trials conducted with selected chemical and biological insecticides in 1998-2000 showed the highest effectiveness of Karate Zeon 100 CS (lambda-cyhalotrine) in European corn borer (ECB) larvae control in sweet corn. The efficacy of biological insecticides containing Bacillus thuringiensis ssp. kurstaki: Biobit 3.2 WP and Lepinox WDG was very variable between the years. Reasons for insufficient efficacy of these products are discussed. The most appropriate time for the application of a chemical insecticide against ECB larvae are plant developmental stages since the beginning of pollen shedding to full blooming (63–67 BBCH scale). The efficacy of treatment was the highest at that time.
Pheromone-responsive plasmids constitute a unique group of ~ 20 plasmids identified, as yet, only among enterococcal species. Several of their representatives, e.g. pAD1, pCF10, pPD1 and pAM373 have been extensively studied. These plasmids posses a sophisticated conjugation mechanism based on response to sex pheromones - small peptides produced by plasmid-free recipient cells. Detailed analysis of regulation and function of the pheromone response process revealed its great complexity and dual role - in plasmid conjugation and modulation of enterococcal virulence. Among other functional modules identified in pheromone plasmids, the stabilization/partition systems play a crucial role in stable maintenance of the plasmid molecule in host bacteria. Among them, the par locus of pAD1 is one of the exceptional RNA addiction systems. Pheromone-responsive plasmids contribute also to enterococcal phenotype being an important vehicle of antibiotic resistance in this genus. Both types of acquired vancomycin resistance determinants, vanA and vanB, as well many other resistant phenotypes, were found to be located on these plasmids. They also encode two basic agents of enterococcal virulence, i.e. aggregation substance (AS) and cytolysin. AS participates in mating-pair formation during conjugation but can also facilitate the adherence of enterococci to human tissues during infection. The second protein, cytolysin, displays hemolytic activity and helps to invade eukaryotic cells. There are still many aspects of the nature of pheromone plasmids that remain unclear and more detailed studies are needed to understand their uniqueness and complexity.
The study presents a new strategy of synthesis of the pine-tree lappet moth sex pheromone via hydrolysis of the (5Z,7E)-1,1-diethoxy-5,7-dodecadiene (propheromone) and their using in biological tests with pheromone traps. The field trials showed that a sticky delta traps with propheromone at dose of 2.4 mg was the most suitable for monitoring of the population of the pine tree lappet moth.
In the recent years, attracting the clearwing moth adults by the sex pheromones has been the most frequently applied method of the collecting. Some practical remarks concerning the use of sex pheromones in faunistic studies on Sesiidae are given.
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