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Genetic control of gonadal development proceeds through either the male or female molecular pathways, driving bipotential gonadal anlage differentiation into a testis or ovary. Antagonistic interactions between the 2 pathways determine the gonadal sex. Essentially sex determination is the enhancement of one of the 2 pathways according to genetic sex. Initially, Sry with other factors upregulates Sox9 expression in XY individuals. Afterwards the expression of Sox9 is maintained by a positive feedback loop with Fgf9 and prostaglandin D₂ as well as by autoregulative ability of Sox9. If these factors reach high concentrations, then Sox9 and/or Fgf9 may inhibit the female pathway. Surprisingly, splicing, nuclear transport, and extramatrix proteins may be involved in sex determination. The male sex determination pathway switches on the expression of genes driving Sertoli cell differentiation. Sertoli cells orchestrate testicular differentiation. In the absence of Sry, the predomination of the female pathway results in the realization of a robust genetic program that drives ovarian differentiation.
Analyses were made of 118 skull measurements of adult wild cats Felis silvestris Schreber, 1777 from Bulgaria, taken on 24 males, 20 females, and 10 animals whose sex was not known in advance. Group (cluster) analysis of cases, factor analysis, and stepvise discriminant analysis were adapted. The cluster analysis of cases indicated a high level of sex mixture (up to 40%), which suggested the importance of the outliers in the data. Six keys to sexual dimorphism, of very high statistical significance were produced, through the stepvise discriminant analysis. They included from 9 down to 1 variables each, which provided from 100% down to 93.8% of correct sex classification of wild cat skulls.
Bream, Abramis brama (L.), eggs fertilized with genetically inactivated sperm (UV irradiation dose of 1920 J m-2) were exposed to thermal cold shock to produce meiotic gynogenotes. The shock was applied at one-minute intervals from 1 to 10 min after egg insemination. The temperature of the shock was 2.0 ± 0.1°C, and its duration was 45 min. The water temperature prior to the shock was 20.0°C. Eggs fertilized with genetically inactivated sperm (putative haploids) exhibited retarded and abnormal development. The yield of gynogenesis was relatively low, except for the group to which the shock was applied 1 min after fertilization (about 30% in comparison with the controls). Ninety fish from the control and gynogenetic groups were reared for ten months. The survival of the gynogenetic bream was twofold lower than that of the controls. The gynogenotes were highly variable in size and exhibited some morphological abnormalities. The sex ratios in the control groups were close to 1:1, whereas all the gynogenotes were female.
Adult males of Schistocerca gregaria turn yellow when they become sexually mature. This is due to the deposition in the cuticle of a male-specific Yellow Protein (YP), of which the amino acid sequence is known. Yellowing only happens in crowd-reared (gregarious) males, and results from the deposition of a specific ‘Yellow Protein’. If individual males (solitarious) are isolated after the adult emergence, they become sexually mature but they do not turn yellow. On the basis of a partial YP-mRNA sequence, we established a reverse transcriptase polymerase chain reaction (RT-PCR) assay to study the developmental expression of YP in crowd-reared males, isolated-reared males and females. In crowd-reared adult males the transcription of YP gene started from day 5 on, and reached a maximum at day 12. The effects of juvenile hormone (JH), insulin (bovine), corazonin, ecdysone and 20 0H-ecdysone (20E) on the regulation of YP-mRNA synthesis were also investigated. JH made the cuticle turn yellow and, as shown by RT-PCR, YP-mRNA was induced. The effect of 100 μg JHIII was stronger than that of 10 μg. Insulin was only effective in inducing YP-mRNA synthesis at high dose (19 μg) and after more days (18 d). Corazonin and 20E made the cuticle turn black, but no YP-mRNA synthesis was observed. Ecdysone (10 and 100 μg) showed no effect on body coloration and YP-mRNA. Thus, JH was found to be the most potent inducer among the hormones tested.
Advanced knowledge of the canine genome facilitates progress in studies on genome maps of other canids, including species considered also as farm animals. In this study canine BAC (Bacterial Artificial Chromosome) probes, harbouring three genes involved in sex determination (SOX9 - sex determining region Y- box 9, AMH - anti-Müllerian hormone and AR - androgen receptor), were mapped in the dog, red fox, arctic fox and Chinese raccoon dog chromosomes. Localization of these genes can be helpful in association studies focused on monogenetic intersexual disorders.
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