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1

Occurrence of Yersinia enterocolitica O:9 in feces from cows seropositive for brucellosis

100%
Szulowski K., Weiner M., Iwaniak W.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 01
The diagnosis of brucellosis is mainly based on serological tests. All animals classified as serologically positive are obligatorily slaughtered and subjected to bacteriological examination. B. abortus has not been reported in Poland since the eradication of bovine brucellosis in 1980. On the other hand, B. suis biovar 2 is sporadically isolated from cattle. In accordance with the instructions of the Chief Veterinary Officer, samples of feces from animals slaughtered following positive serological results for brucellosis have been examined for the presence of Yersinia enterocolitica O:9 since 2011. Because of the similarity of its O-polysaccharide component of S-LPS with that of Brucella, this microorganism is considered to be a major contributory factor of false positive serological reactions (FPSR). The paper presents the results of the bacteriological and molecular examination of feces from 26 cows seropositive for brucellosis and 30 healthy cows, negative for brucellosis, for the presence of Y. enterocolitica O:9. Y. enterocolitica O:9 was found in 7 of the positive cows, whereas all samples from cows negative for brucellosis were free from this bacteria. These results indicate that Y. enterocolitica O:9 may be responsible for some of the positive results for brucellosis in cattle.
2

Characterization of the first Polish isolate of Neospora caninum from cattle

100%
Gozdzik K., Cabaj W.
Acta Parasitologica
|
2007
|
tom 52
|
nr 3
Neospora caninum was isolated from the brain of an apparently healthy calf born to a seropositive cow. The calf was killed 12 hrs after birth and homogenate of its brain was inoculated into Vero cells. Neospora-like tachyzoites were detected 66 days later. The identity of the parasite was confirmed by polymerase chain reaction amplification of N. canium-specific fragments using Np21 and Np6 primers. This first Polish isolate of N. caninum was designated NC-PolB1.
3
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The use Limousin bull semen reduced the risk of abortion in Neospora seropositive dairy cows

80%
Garcia-Ispierto I., Lopez-Gatius F., Santolaria P., Yaniz J., Nogareda C., Pabon M., Lopez-Bejar M., Almeria S.
Wiadomości Parazytologiczne. Suplement
|
2005
|
tom 51
4

Polymerase chain reaction (PCR) detection of Neospora caninum DNA in oocytes and embryos of seropositive cows

80%
Moskwa B., Bien J., Gozdzik K., Cabaj W.
Wiadomości Parazytologiczne. Suplement
|
2008
|
tom 54
5

The suitability of milk in detection of Neospora caninum infection in cows

80%
Moskwa B., Cabaj W., Pastusiak K., Bien J.
Acta Parasitologica
|
2003
|
tom 48
|
nr 2
Neospora caninum, an apicomplexan protozoan causes economical losses due to reproductive failure associated with abortion among cattle. The diagnosis of neosporosis is difficult, due to nonspecific clinical signs and there are no parasitological examinations which can help to recognise neosporosis in vivo. Milk of seropositive cows was examined for both, IgG level to antigens of N. caninum and the presence of DNA of the parasite. Our results revealed that a milk sample from a seropositive cow diluted from 1:2 to 1:8 showed more than five times higher IgG level against N. caninum antigen when compared with a seronegative sample. Additionally, PCR test with Np6 and Np21 primers gave a 328 bp product in examined samples and confirmed the presence of N. caninum DNA in milk of seropositive cows. Data confirmed that vertical transmission of N. caninum from dams to older animals, in the late or post lactation period by milk should be taken into account. Moreover, the milk could be suitable in detection of N. caninum infection in cows using two complementary methods: ELISA and PCR.
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