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The study included 260 hospitalised children with suspected infection with human ascaris. In serological diagnostics a protein antigen obtained from Ascaris lumbricoides was used. ELISA method was applied. IgG antibodies were detected. Positive results were found in 15% of the examined children. No relation to the gender or demographic conditions was found. The most frequently observed symptoms in the patients with Ascaris lumbricoides were: abdominal pain - 87%. diarrhoea 15%. In 31% of the cases eosinophilia was found. Scatoscopy was carried out for all the patients, using the PARASEPT system and Kato and Miura methods as well as decantation and tlotation. The examination. which was repeated three times, did not show cysts or eggs. Serological investigation exhibits higher sensitivity than the traditional methods. Their use in recognising ascariasis in humans significantly facilitates diagnostic procedures, especially in the lung phase of the disease, the larval stage or in cases of infection with an individual parasite, when the faeces samples do not contain the eggs. Serological investigation is also useful in all cases of suspected VLM.
Brucellosis was confirmed in boars imported for breeding purposes to Poland from one of the EU countries. In serological investigations, in which the RBT, ELISA, CFT, SAT and 2-ME were used, positive reactions to brucellosis were found in 9 out of 23 animals. All the animals were slaughtered, and bacteriological examination was performed. Brucellae were isolated from the tissues of 7 boars. The characteristics of the strains isolated showed that they belonged to Brucella suis biovar 2, which is typical for Europe. The examination of animals for brucellosis at quarantine stations seems to be crucial for the protection of herds from Brucella infection.
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