The research was conducted on weed infestation of potato crops in fields with different soils in several (9) municipalities in the Zamość region in 2014–2015 were compared with the results of research carried out in 1991–1995. The phytosociological photos were created by Braun-Blanquet’s method, of which 87 representative images were selected for analysis. The photos were taken on three complexes of agricultural suitability of soils: good wheat, defective wheat and good rye. Generally identified 55 species of weeds (43 annual and biennial and 12 perenial). The cultivation of potato less were infested on the complex good wheat and defective than good rye. One photo in the first research period (1991–1995) accounted from 45 to 52 species, while in the second period (2014–2015) from 41 to 49 species. In potato canopy dominated nitrophilous species: Chenopodium album, Echinochloa crus-galli, Matricaria maritima subsp. inodora, Stellaria media, Galinsoga parviflora, Elymus repens and Equisetum arvense. In general, irrespective of the soil complex, the resumption of testing after 20 years in selected locations in various municipalities of the Zamość region indicates is very limited of certain taxa, such as Galeopsis tetrahit, Veronica persica, Papaver rhoeas, Avena fatua, Sinapis arvensis, Spergula arvensis, Fallopia convolvulus and Polygonum persicaria, in addition to Galium aparine and Stellaria media on poorer soil complexes. An increase in infestation of potato was noted for Chenopodium album, Echinochloa crus-galli, Setaria pumila, Anthemis arvensis, Atriplex patula and Lamium purpreum. Among perennial taxa, crops are infested in the highest degree by Elymus repens and Equisetum arvense.
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