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The experiment was conducted in the years 2001–2003 at the Experimental Station in Złotniki. The aim of the performed investigations was to evaluate economic effectiveness of different fungicidal protection programs in winter wheat. Winter wheat of cv. Sakwa was cultivated using the following two variants of seed treatment: 1) Raxil 060 FS at the dose of 60 ml/100 kg grain, 2) Raxil 060 FS + Latitude 125 FS at the doses of 60 and 200 ml/100 kg, and five variants of fungicidal foliar protection: 1) Vista 228 SE, 2) Sportak Alpha 380 EC, 3) Sportak Alpha 380 EC + Vista 228 SE, 4) Sportak Alpha 380 EC + Vista 228 SE + Juwel 250 SC, 5) control – without protection. The use of the above plant protection products contributed to the increase of winter wheat grain yield from 0.60 t/ha to 2.07 t/ha. This increase of yield covered costs of performed chemical control. The economic analysis showed that most effective variant of winter wheat chemical protection was seed treatment with Latitude 125 FS with additional two foliar treatments with the following fungicides: Sportak Alpha 380 EC and Vista 228 SE. Irrespective of the applied seed dressing, additional application of Juwel 250 SC at the stage of early milk maturity turned out to be economically not justified.
In the Institute of Plant Protection Branch in Sośnicowice an investigation was performed on establishing novel methods for testing biological activity or seed dressings against snow mould (Microdochium nivale). Seeds of winter wheat used for the experiments in artificial conditions were disinfected in sodium hypochlorite, subsequently infected with the pathogen, and treated with seed dressing fungicides (Baytan Universal 19.5 WS, Zaprawa Funaben T, Maxim 025 FS, Raxil Extra 515 FS and Vitavax 200 FS). In laboratory tests inoculation with conidial suspension was applied, and in climatic chamber experiments either conidial suspension or inoculum grown on sand-cornmeal medium was used. The field experiment with the same seed dressings was carried out on naturally infested plots, and the disease developed under snow cover lasting for 58 days. Two novel climatic chamber methods (Z and PK) proved to be suitable for testing biological activity of seed dressing fungicides against snow mould (M. nivale) as correlation coefficients with field results at P=0.05 were 0.9760 and 0.9453. Method Z was recognised as more suitable. Differences between seed dressings were statistically insignificant. The laboratory method was recognised as useful for controlling the pathogen (M. nivale), but its usefulness for controlling the disease (snow mould) may not be sufficient under a range of different winter conditions.
In the years 1992-94 studies were carried out on the occurrence of the broad bean weevil at the broad bean plantations and evaluation of the effect of insecticidal seed dressings upon this pest. The first beetles of the broad bean weevil begin their flight over the plantation before plant flowering (the 3rd decade of May, the 1st decade of June). A maximum of the laid out eggs was noted in the 2nd and 3rd decades of July. The best effects of the pest control were obtained by applying the insecticidal seed dressing Gaucho 350 FS-and by spraying plants at the pod setting phase with insecticide Karate 025 EC. The performed treatments reduced the seed damage of field bean by, on average, 16.6% and increased the yield by, on average, 0,62 t per ha.
In vitro antifungal activity assay of different concentrations of 2-hydroxy-4-methoxybenzaldehyde isolated from Decalepis hamiltonii against six important seed-borne fungal pathogens viz., Alternaria alternata, Drechslera tetramera, Fusarium oxysporum, F. proliferatum, Pyricularia oryzae and Trichoconis padwickii isolated from paddy seeds revealed that, the compound 2-hydroxy-4-methoxybenzaldehyde showed significant antifungal activity. Among the fungi tested, F. proliferatum showed highest inhibitory activity, whereas P. oryzae showed least inhibitory activity. The minimal inhibitory concentration (MIC) varied between 350 μg/ml and 650 μg/ml depending on the fungal species. Comparative evaluation of the active compound with the synthetic fungicide thiram at recommended dosage revealed that, the antifungal activity of the active compound obtained from the plant was almost equivalent. Evaluation for nutritional parameters and dry matter losses (DML) revealed that, total carbohydrates, water soluble proteins, lipids and dry matter losses were significantly confined in 2-hydroxy-4-methoxybenzaldehyde treated paddy seeds compared with control seeds. This plant being an edible one can be exploited in the management of seed-borne pathogenic fungi and in the prevention of biodeterioration of grains and mycotoxin production during storage in an eco-friendly way.
The aim of presented experiments was to determine the usefulness of plant extracts for dressing of cereal seeds. Water extracts (macerations, infusions) prepared from different morphological parts of 39 plant species were used as dressing preparations. Non-disinfected seeds of two cultivars of barley (Hordeum vulgare L.): Rudzik (brewing barley) and Stratus (common barley) were used for the purpose of dressing. The experiment was conducted as a filter paper test (PN-94 R-65950) while determining the germination viability (1st date) and the germination capacity (2nd date) as well as the healthiness of the seeds. A significant differentiation of the effect of the extracts on vitality and healthiness of seeds was demonstrated depending on the origin of the extract (plant species), the way it was prepared and an interaction between these factors. The germination viability of seeds of brewing barley was influenced mostly by infusions from fruits of Coriandrum sativum, from seeds of Linum usitatissimum, from bark of Quercus robur, from roots of Levisticum officinale, from roots of Arctium lappa and from flowers of Verbascum thapsiforme. The most favourable activity on germination capacity was revealed using the infusions from seeds of L. usitatissimum, from roots of A. lappa, from fruits of C. sativum and macerations from roots of A. lappa, from fruits of C. sativum and from bark of Q. robur. The infestation of seeds by microflora was reduced by macerations from leaves of Betula verrucosa, from seeds of L. usitatissimum, from fruits of Juniperus communis, and infusions from flowers of Crataegus oxyacantha, from seeds of L. usitatissimum, from leaves of B. verrucosa. The most favourable impact on viability of the seeds of common barley was revealed for infusions from roots of L. officinale, from stigmas of Zea mays, from flowers of C. oxyacantha and macerations from flowers of Lavandula vera, from leaves of Mentha piperita and from roots of L. officinale. A positive effect on the germination capacity was exerted by infusions from the stigmas of Z. mays, from flowers of C. oxyacantha, from rhizomes of Acorus calamus, from bark of Frangula alnus, and macerations from bark of F. alnus, from leaves of M. piperita, from flowers of C. oxyacantha and from herb of Marrubium vulgare. The contamination of the seeds by microflora was reduced most effectively with infusions from young sprouts of Pinus sylvestris, from roots of Inula helenium, from leaves of Juglans regia, from herb of M. vulgare, from stigmas of Z. mays and macerations from rhizomes of A. calamus, from herb of M. vulgare, from leaves of J. regia, from seeds of L. usitatissimum and from flowers of L. vera.
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