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A non-soil seed bank dependent on the size of clonal plants: the case of Carex cespitosa, a guerrilla species in an unknown meadow

100%

Borkowska L., Lembicz M., Kasprzykowski Z.

Polish Journal of Ecology

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2017

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tom 65

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nr 2

Long-lived clonal plants provide an optimal place for the natural storage of seeds as a ‘non-soil seed bank’. We tested the hypothesis that the size and species diversity of a non-soil seed bank deposited within the clonal plant Carex cespitosa depends mainly on the plant's size. To verify this hypothesis, an experiment was conducted on an unmown meadow in the Białowieża National Park. The emerging seedlings of different species originating from C. cespitosa tussocks of different size (large and small) were observed under greenhouse conditions for four years. The size of a non-soil seed bank was evaluated based on the number of seedlings. Significant differences in the seedling number were found between large and small sedge tussocks (Mann-Whitney test Z = 3.96, P < 0.001). However, the number of recorded species was independent of tussock size. Both types of tussocks were dominated by meadow, forest and swamp species (in decreasing order). Some of these species are no longer present in the studied area, or their occurrence is limited to small groups at the meadow's edges. A non-soil seed bank within the tussock cores of C. cespitosamay be, similar to a soil seed bank, a tool for studying the changes occurring in plant communities.

2

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Betula pendula seed storage and sowing pre-treatment: effect on germination and seedling emergence in container cultivation

100%

Tylkowski T.

Dendrobiology

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2012

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tom 67

Irregular seed crop in Betula pendula (Silver Birch) is a reason to create seed reserves, ensuring nursery production in years of crop failure. This study investigated the effects of seed moisture content and storage temperature on germinability and seedling emergence in container cultivation. Mature catkins were collected separately from 3 trees. The mixture of winged nuts and scales was dried to 3 levels of moisture content and stored at 3°C, –3°C and –10°C. After storage for 3, 5 and 6 years, the seeds were separated from scales and next dewinged and sorted in acetone into empty and filled seeds. Cleaned nuts were germinated in the Jacobsen germinator. Besides, to assess seedling emergence, seeds were sown on the surface of peat mixed with perlite, in multi-cell trays under a plastic tunnel. Significant differences in germinability were detected depending on the mother tree, seed moisture content and storage time. The viability of seeds stored at –10°C remained unchanged for 6 years regardless of moisture content (ca. 8–12%). Seeds stored at higher temperatures lost their germinability faster. An unexplained increase in seedling emergence was observed after extended seed storage at –10°C, in contrast to a gradual decrease in seedling emergence after extended storage at –3°C.

3

Selected aspects of tiny vetch [Vicia hirsuta (L.) Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination

100%

Kucewicz M., Mackiewicz K., Zrobek-Sokolnik A.

Acta Agrobotanica

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2010

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tom 63

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nr 1

Vicia hirsuta (L.) Gray S.F. (tiny vetch) is a common and persistent segetal weed. Tiny vetch seeds and pods reach different stages of maturity during the crop harvest season. Some seeds that mature before cereal harvest are shed in the field and deposited in the soil seed bank, while others become incorporated into seed material. The objective of this study was to describe selected aspects of tiny vetch seed ecology: to determine the rate of individual reproduction of vetch plants growing in winter and spring grain crops and to evaluate the germination of seeds at different stages of maturity, subject to storage conditions. The seeds and pods of V. hirsuta were sorted according to their development stages at harvest and divided into two groups. The first group was stored under laboratory conditions for two months. In the autumn of the same year, the seeds were subjected to germination tests. The remaining seeds were stored in a storeroom, and were planted in soil in the spring. The germination rate was evaluated after 8 months of storage. Potential productivity (developed pods and flowers, fruit buds) was higher in plants fruiting in winter wheat than in spring barley. Vetch plants produced around 17-26% more pods (including cracked, mature, greenish-brown and green pods) and around 25% less buds in winter wheat than in spring barley. Immature seeds were characterized by the highest germination capacity. Following storage under laboratory conditions and stratification in soil, mature seeds germinated at a rate of several percent. After storage in a storeroom, seeds at all three development stages broke dormancy at a rate of 72- 75%. The high germination power of tiny vetch seeds stored in a storeroom indicates that this plant can be classified as an obligatory speirochoric weed species.

4

Some phytopathological aspects of Scots pine (Pinus sylvestris L.) seed storage

100%

Kowalczyk A.

Phytopathologia Polonica

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2007

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tom 45

5

GGT-assisted glutathione degradation is functional to apoplastic redox control and seed storage proteins accumulation

84%

Trentin A., Pivato M., Giaretta S., Fabrega-Prats M., Arrigoni G., Prasad D., Masi A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

6

Hydrotime model analysis of cornflower achene germination

84%

Bochenek A., Golaszewski J., Kondrat B., Szymczak M., Jagielska T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

7

Symulowanie warunkow przechowywania nasion rzepaku w silosach

84%

Tys J., Szwed G.

Rośliny Oleiste - Oilseed Crops

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1997

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tom 18

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nr 2

451-457

8

Silencing the [alpha]-gliadins in wheat

84%

Folck A., Wieser H., Lorz H., Becker D.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

9

Alpha-D-galactosidase activity in stored yellow lupin [Lupinus luteus L.] seeds

84%

Lahuta L. B., Gorecki R. J., Michalczyk D., Piotrowicz-Cieslak A. I.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

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2000

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tom 03

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nr 1

The activity of alpha-D-galactosidase and contents of soluble saccharides were studied in Juno yellow lupin (Lupinus luteus L.) seeds stored. Seeds harvested at three stages of maturity (15, 25, 35 days after flowering - DAF) were stored at 21°C for two years (frozen immediately after harvest) or were dried after harvest to 8% of water content and stored at +20°C for 5 and 6 years. A high alpha-D-galactosidase activity in maturing and mature seeds could have been one of the causes of decreasing viability and vigour of the seeds stored. The hydrolysis of raffinose oligosaccharides decreased the ratio of these saccharides to sucrose. The decrease in the content of raffinose oligosaccharides was accompanied by an increase in galactosyl cyclitol contents.

10

On the geographic variation of Ciboria batschiana (Zopf) Buchwald, the main pathogenic fungus on acorns of Quercus robur and Q.petraea in Europe

84%

Schroder T.

Dendrobiology

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2002

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tom 47 Supplement

Mycelial growth of the pathogenic fungus Ciboria batschiana (Zopf) Buchwald was examined at nine temperature levels between -3°C and +35°C using pure cultures of the fungus from eleven different provenances located in Germany, Poland, the Czech Republic and Slovenia. The fungus was isolated mainly from cotyledons of acorns of the oak species Quercus robur L. and Quercus petraea (Matt.) Liebl. Even at -3°C the mycelium of the fungus grew well. There were significant differences between the provenances in respect to growth rate and temperature tolerance, especially at low temperatures.

11

Generative propagation of Harlequin flower [Sparaxis tricolor [Curt] Ker.Gawl]

84%

Kapczynska A., Piskornik M.

Folia Horticulturae

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2003

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tom 15

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nr 2

12

Picea abies in Northrhine-Westphalia

67%

Schmitt H. P., Heyder J.

Dendrobiology

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2009

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tom 61 Supplement

The Sauerland region of southern Westphalia has the most economic stands of Norway spruce in the state of Northrhine-Westphalia (Germany). As shown by historical research and genetic analysis, the seeds for these stands were imported from Thuringia around the year 1880. Therefore, the two states are collaborating closely to secure the genetic variety of the spruce and develop new sources of seed supply. The survival of the Hochsauerland Norway spruce is now under threat: these stands have already been harvested or are due for harvesting shortly, in addition, many of the older stands were destroyed by the gale "Kyrill" in 2007, and only a few stands have remained. Since its foundation, the Forest Gene Bank of Northrhine-Westphalia has taken the following measures to preserve the gene pool of the Hochsauerland spruce and conserve it in the long term: storage of seed from 100 representative trees in all the most valuable stands of spruce (since 1985); establishment of seed orchards; and establishment of replacement stands. Thanks to those measures, the genetic information of the Norway spruce stands of Thuringian origin has been secured, and substantial stocks of seed are available for reforestation.

13

Sprawozdanie Naukowego Instytutu Buraka Cukrowego w Getyndze

67%

Gazeta Cukrownicza

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1989

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tom 97

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nr 06

14

Seed storage, germination and seedling emergence in Rhamnus catharticus

67%

Tylkowski T.

Dendrobiology

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2007

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tom 58

67-72

This study was aimed to improve the understanding of germination ecology and to explain the invasive character of the common buckthorn (Rhamnus catharticus) in North America. Its fully mature seeds are characterized by a lack of dormancy. In laboratory conditions, favourable thermal conditions were identified for seed storage, germination and seedling emergence. At the cyclically alternating temperature of 20~30°C (16+8 h daily), seeds of this species showed a high germination rate within few weeks. Two other thermal variants, 3~15°C and 3~20°C (16+8 h daily), were much less effective for seed germination. However, cold stratification (at 3°C) in a moist mixture of peat and sand, lasting 4–8 weeks, caused a remarkable increase in germination rate also at 3~15°C. Seeds extracted from ripe fruits and dried (to a moisture content of about 10%) showed high germination and emergence rates after storage for 3.5 years at –3°C.

15

Phenotypic variation in relation to seed storage protein polymorphism in Bromus secalinus L. (Gramineae) populations from North-Eastern Poland

67%

Skrajna T., Kubicka H., Rzymowska Z.

Polish Journal of Ecology

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2012

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tom 60

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nr 1

Bromus secalinus L. is an annual speirochoric species growing in agrocenoses of winter crops. In the past, B. secalinus L. could be found throughout Poland. The regression of the species has caused it to be classified as a rare species threatened with extinction. The study was conducted in the 2007–2010 time period, in north-eastern Poland where a considerable increase in the number of sites and abundance of B. secalinus were observed. The plant material was collected from agrocenoses of winter crops, at 14 sites representing the habitat diversity of the species occurrence. Eight morphological features were taken into account when evaluating populations of B. secalinus. The morphological diversity of the populations of B. secalinus was correlated with different habitat conditions (type, pH of soil and cultivated plant). The populations growing on the sites with optimum trophic and moisture conditions as well as those populations growing in very poor conditions were found to differ the most. A comparison of B. secalinus was done and six groups of cluster similarities were found using the agglomeration method Electrophoretic analysis of seed storage proteins of B. secalinus L from the studied populations were performed using SDS-PAGE. Analysis revealed relatively high genetic polymorphism irrespective of whether genetic variation was compared between the populations from different sites or between the subpopulations from only site number 4. Three homologenous groups were formed which were related to the cultivated plant coverage, and partially, to the type of soil. In the investigated populations and subpopulations, protein bands of molecular weight from 8 to 68 kDa were observed. The SDS-PAGE method was found suitable for the assessment of genetic variation in populations of B. secalinus L from many sites as well as within the subpopulations from one site.

16

Kinetyka zmian zawartości ergosterolu podczas przechowywania nasion rzepaku

67%

Gawrysiak-Witulska M., Wawrzyniak J., Stuper K., Rusinek R.

Acta Agrophysica

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2015

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tom 22

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nr 1

Ergosterol jest najważniejszym sterolem tworzonym przez większość grzybów i jest stosowany jako chemiczny wskaźnik obecności zasiedlających grzybów. Celem pracy było zbadanie wpływu temperatury i wilgotności nasion rzepaku na ich jakość mikrobiologiczną. W pracy oceniano skażenie mikrobiologiczne rzepaku na podstawie poziomu stężenia ergosterolu. Materiałem badawczym były nasiona rzepaku odmiany Californium przechowywane w komorze termostatycznej (25±1 i 30±1°C) wyposażonej w trzy aparaty higrostatyczne służące do utrzy-mania wilgotności względnej powietrza na stałym poziomie. Przed rozpoczęciem eksperymentu rzepak nawilżano do wilgotności 10,5; 12,5; 15,5% (w.b). Nasiona przechowywano do czasu, aż ich zdolność kiełkowania obniżyła się poniżej 75%. Podczas przechowywania, co 6 dni, pobierano próby do badań. Przeprowadzone badania wykazały istotny wpływ wilgotności nasion i temperatury przechowywania na przyrost stężenia ergosterolu.

17

Wielkość i kształt jako cechy rozdzielcze nasion dębu szypułkowego (Quercus robur L.)

67%

Tylek P.

Acta Agrophysica

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2012

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tom 19

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nr 3

Uzyskanie kwalifikowanego materiału siewnego wymaga wykonania szeregu czynności fizycznych, mających źródło w znajomości praw rządzących procesami rozdzielczymi. Podstawą ich są różnice w niektórych właściwościach fizyko-mechanicznych pomiędzy poszczególnymi składnikami mieszanin ziarnistych. Znajomości takiej wymaga ponadto konstruowanie podzespołów maszyn i urządzeń do uszlachetniania materiału siewnego oraz efektywna eksploatacja czyszczalni i sortowników mechanicznych oraz innych maszyn do obróbki nasion. Zarówno współczesne jak i dawne konstrukcje separatorów są oparte na doświadczeniu praktyków, a nie teoretycznych koncepcjach mających źródło w gruntownej znajomości agrofizyki nasion. Ze względu na powyższe niezbędna jest szczegółowa analiza cech rozdzielczych dębu, uwzględniająca także cechy nie wykorzystywane w leśnictwie do tej pory, a które powinny być wzięte pod uwagę w konstrukcji przyszłościowych rozdzielaczy uniwersalnych. Mechaniczna separacja nasion wymaga znajomości ich podstawowych parametrów geometrycznych, w tym wielkości i kształtu. Pomiary wspomnianych parametrów wykonano korzystając z komputerowej analizy obrazów nasion, uzyskanych z aparatu cyfrowego. Celem pracy była analiza wielkości i kształtu żołędzi dębu szypułkowego (Quercus robur L.) opisana współczynnikami kształtu, z uwzględnieniem przynależności do klas żywotności. Przyjęto klasyfikację nasion na 3 frakcje pomiarowe, biorąc za podstawę rozwój zarodka oraz szacowaną na tej podstawie spodziewaną zdolność kiełkowania nasion. Wyniki badań wykazały, że cechy geometryczne żołędzi nie mogą być uznane za cechy rozdzielcze, zatem nie ma możliwości przeprowadzenia procesu separacji w oparciu o wielkość nasion. Podczas badań zaobserwowano także, że tradycyjny sposób suszenia żołędzi, celem przygotowania do przechowywania, nie umożliwia uzyskania jednorodnej wilgotności końcowej. Jedynie nasiona żywotne o dużych zarodkach wykazują właściwą wilgotność, znacząco wyższą od pozostałych. Stanowi to zapewne jedną z przyczyn spadku zdolności kiełkowania nasion w trakcie przechowywania.

18

Rzepak – jakość nasion, procesy zbioru, suszenia, przechowywania

67%

Tys J., Rybacki R.

Acta Agrophysica

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2001

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tom 44

19

Studies on genetic changes in rye samples [Secale cereale L.] maintained in a seed bank

67%

Chwedorzewska K. J., Bednarek P. T., Lewandowska R., Krajewski P., Puchalski J.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 3

The aim of this study was to identify genetic changes in rye seeds induced by natural ageing during long-term storage and consecutive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples varying in their initial viability after one and three cycles of reproduction was analyzed by AFLP (amplified fragment length polymorphism) fingerprinting. Seven EcoRI/MseI primer combinations defined 663 fragments, and seven PstI/MseI primer combinations defined 551 fragments. The variation in the frequency of the seventy-four EcoRI/MseI bands was statistically significant between samples. These changes could be attributed to genetic changes occurring during storage and regeneration. However, the PstI/MseI fragments appeared to be uninfluenced by seed ageing, regeneration and propagation. A combined Principle Coordinate Analysis revealed differences between samples with different initial viability. We showed that materials with low initial viability differ in their response from highly viable ones, and that the changes exhibited in the former case are preserved through regeneration cycles.

20

Effect of seed storage time on the photosynthetic activity of Quercus robur, Fagus sylvatica, Tilia cordata, and Fraxinus excelsior seedlings

67%

Lorenc-Plucinska G., Preuss A., Michalak A., Stobrawa K., Plucinski P., Tylkowski T., Suszka J., Chmielarz P.

Biological Letters

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2005

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tom 42

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nr 2 Spec.Vol.

Ograniczanie wyników

A non-soil seed bank dependent on the size of clonal plants: the case of Carex cespitosa, a guerrilla species in an unknown meadow

Betula pendula seed storage and sowing pre-treatment: effect on germination and seedling emergence in container cultivation

Selected aspects of tiny vetch [Vicia hirsuta (L.) Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination

Some phytopathological aspects of Scots pine (Pinus sylvestris L.) seed storage

GGT-assisted glutathione degradation is functional to apoplastic redox control and seed storage proteins accumulation

Hydrotime model analysis of cornflower achene germination

Symulowanie warunkow przechowywania nasion rzepaku w silosach

Silencing the [alpha]-gliadins in wheat

Alpha-D-galactosidase activity in stored yellow lupin [Lupinus luteus L.] seeds

On the geographic variation of Ciboria batschiana (Zopf) Buchwald, the main pathogenic fungus on acorns of Quercus robur and Q.petraea in Europe

Generative propagation of Harlequin flower [Sparaxis tricolor [Curt] Ker.Gawl]

Picea abies in Northrhine-Westphalia

Sprawozdanie Naukowego Instytutu Buraka Cukrowego w Getyndze

Seed storage, germination and seedling emergence in Rhamnus catharticus

Phenotypic variation in relation to seed storage protein polymorphism in Bromus secalinus L. (Gramineae) populations from North-Eastern Poland

Kinetyka zmian zawartości ergosterolu podczas przechowywania nasion rzepaku

Wielkość i kształt jako cechy rozdzielcze nasion dębu szypułkowego (Quercus robur L.)

Rzepak – jakość nasion, procesy zbioru, suszenia, przechowywania

Studies on genetic changes in rye samples [Secale cereale L.] maintained in a seed bank

Effect of seed storage time on the photosynthetic activity of Quercus robur, Fagus sylvatica, Tilia cordata, and Fraxinus excelsior seedlings