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Chromatographic analysis of rapeseed glucoside fractions

100%

Amarowicz R., Kozlowska H., Shimoyamada M., Okubo K.

Polish Journal of Food and Nutrition Sciences

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1992

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tom 01

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nr 1

2

Simplification of a haemolytic micromethod for toxic saponin quantification in alfalfa

88%

Gorski P. M., Jurzysta M.

Acta Agrobotanica

|

1988

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tom 41

|

nr 2

A simplification of a haemolytic micromethod is presented. In the orginal method, alfalfa (Medicago media Pers.) leaf sap is spotted on the plate covered with a blood-gelatine suspension. In the presented method, the mashed alfalfa pulp is used instead of sap. Due to saponin diffusion and the reaction with erythrocytes, a haemolytic ring appears, which has a width proportional to the concentration of toxic saponins. It is shown that the width of haemolytic ring does not depend on the sample weight ranging from 20 to 100 mg. This allows for the omission of laborious sap pressing and sample quantification. Individual alfalfa plants with different saponin contents were tested using leaf sap and leaf pulp for analyses. Good agreement was found with sap and leaf pulp methods. The correlation obtained by both methods was high, r = 0.87. The modified method requires only a small amount of plant material and makes the analyses of large numbers of individual plants per day possible. The method is especially recommended for breeding purposes.

3

Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiflorum A.DC.

75%

Urbanska N., Nartowska J., Skorupska A., Ruszkowski D., Giebultowicz J., Olszowska O.

Herba Polonica

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2009

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tom 55

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nr 3

103-108

The sum of saponins in the hairy root lines (6, 17) of Platycodon grandiforum A.DC. was compared. Hairy root line 6 showed a higher total saponin content (6.92%) than the line 17 (6.01%). According to the Chinese Pharmacopoeia standards the content of saponins in Platycodi Radix should be not less than 2%. Our results seem to indicate that the hairy root culture of Platycodon grandiforum A.DC. is a good source of saponins. The Haemolytic Index of the hairy root line 6 was 1600. Digitonin was used as a reference. Moreover, the haemolytic activity of TLC subfractions of saponins varied.

4

Analysis of gypsogenin saponins in homeopathic tinctures

75%

Szakiel A., Henry M.

Acta Biochimica Polonica

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2007

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tom 54

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nr 4

853-856

A relatively simple and short procedure for the quantitative determination of gypsogenin saponins was performed to evaluate homeopathic tinctures in which those compounds can be regarded as one of the active constituents. This method comprises partial hydrolysis of saponins, subsequent extraction of liberated prosaponin (gypsogenin 3-O-glucuronide) and its analysis by high performance liquid chromatography. Glycyrrhizic acid was used as an internal standard. This method was successfully applied to the analysis of mother tinctures obtained from Saponaria officinalis. Thus, the determination of triterpenoid saponins can be used as a convenient and sufficient method of standardization of selected homeopathic tinctures

5

Metoda oznaczania saponin w niektórych środkach spożywczych

75%

Andrzejewska M.

Roczniki Państwowego Zakładu Higieny

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1975

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tom 26

|

nr 1

6

The ability of Candida maltosa for hydrocarbon and emulsified hydrocarbon degradation

75%

Chrzanowski L., Kaczorek E., Olszanowski A.

Polish Journal of Environmental Studies

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2006

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tom 15

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nr 1

The yeast strain Candida maltosa EH 15 was used as a biological agent in the hydrocarbon and emulsified hydrocarbon biodegradation. Six different surface active compounds were used to emulsify hydrocarbons. Biodegradation degree and biomass quantity were determined daily over 7 days. The obtained results indicated the strong ability of Candida maltosa EH 15 for hydrocarbon biodegradation. The addition of the tested surfactants influenced hydrocarbon biodegradation; however, biodegradation effectiveness varies with the type and concentration of surfactant.

7

The effect of alfalfa [Medicago sativa] saponins on Botrytis tulipae and Phoma narcissi growth

75%

Saniewska A., Bialy Z., Jurzysta M.

Phytopathologia Polonica

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2003

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tom 27

8

The influence of alfalfa saponins and mustard glucosinolates on the fertility of Colorado potato beetles [Leptinotarsa decemlineata Say]

75%

Waligora D.

Journal of Plant Protection Research

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2002

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tom 42

|

nr 4

9

Effect of alfalfa [Medicago sativa L.] saponins on development and fertility of grain weevil [Sitophilus granarius L.]

75%

Wawrzyniak M., Blazejewska A., Jurzysta M.

Acta Scientiarum Polonorum. Agricultura

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2003

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tom 02

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nr 2

10

The effect of Medicago arabica, M. hybrida and M. sativa saponins on the growth and development of Fusarium oxysporum Schlecht f. sp. tulipae apt.

63%

Jarecka A., Saniewska A., Bialy Z., Jurzysta M.

Acta Agrobotanica

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2008

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tom 61

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nr 2

In the present work it was shown that total saponins originated from M. hybrida and M. sativa substantially limited mycelium growth of F. oxysporum f. sp. tulipae and symptoms of fusariosis on tulip bulbs. Out of 15 individual tested saponins originated from M. arabica, M. hybrida and M. sativa, four compounds: 3-O-[β-D-glucopyranosyl (1→2)α-L-arabinopyranosyl] hederagenin, hederagenin 3-O-β-D-glucopyranoside, medicagenic acid, medicagenic acid 3-O-β-D-glucopyranoside had the strongest inhibitory effect on mycelium growth of Fusarium oxysporum f. sp. tulipae on PDA medium. The total saponins from M. arabica, M. hybrida and M. sativa inhibited the number of colony forming units of Fusarium oxysporum f. sp. tulipae in artifi cially infested substrate. The use of saponins originated from Medicago as a fungicide is suggested.

11

The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor

63%

Urbanska N., Giebultowicz J., Olszowska O., Szypula W. J.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

|

nr 3

The growth and saponin accumulation were measured in two lines of transgenic hairy roots of Platycodon grandiflorum, Pl 6 and Pl 17, cultured for 8 weeks in 250-ml shake flasks containing 50 ml of hormone-free woody plant medium supplemented with 40 g/l sucrose and in the Pl 17 line cultured for 12 weeks in a 5-l mist bioreactor containing 1.5 l of the same medium. With both methods, the growth of transgenic hairy roots was assessed as both fresh and dry weight and the biomass growth was correlated with the conductivity and sucrose uptake. The accumulation of saponins was measured and compared with that in roots derived from the field cultivation. The saponin concentrations were significantly higher in the two hairy root lines cultured in shake flasks [6.92 g/100 g d.w. (g%) and 5.82 g% in Pl 6 and Pl 17, respectively] and the line cultured in the bioreactor (5.93 g%) than in the roots derived from the field cultivation (4.02 g%). The results suggest that cultures of P. grandiflorum hairy roots may be a valuable source for obtaining saponins.

12

Activity of the saponin extract from the bark of Quillaja saponaria Molina, against Colorado potato beetle [Leptinotarsa decemlineata Say]

63%

Waligora D.

Journal of Plant Protection Research

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2006

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tom 46

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nr 2

199-206

The paper reports results of investigations concerning the influence of saponin extract of quillaja on the food choice and development of Colorado potato beetle. Choice tests with the use of potato leaf discs treated and untreated with tested saponins’ extract were made. The influence of the presence of saponins on the food choice and intensity of insect feeding were observed. It was stated that saponins significantly restrain feeding and disturb development of this pest

13

Effect of platycodin D on gastric motility, gastric emptying and gastrointestinal transit

63%

Kim T.-W., Lim J.-H., Lee H.-K., Giorgi M., Owen H., Yun H.-I.

Medycyna Weterynaryjna

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2014

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tom 70

|

nr 01

Diarrhea is a condition which causes malabsorption and dehydration. Recently, the anti-motility effect of several herbal compounds for the treatment of hypermotility-induced diarrhea has been studied. The root of Platycodon grandiflorum has been widely used in oriental medicine for the treatment of various chronic inflammatory diseases. The aim of the present study was to assess the effects of Platycodin D (PD), the major triterpene saponin in the root of P. grandiflorum, on gastrointestinal (GI) motility by assessing both gastric emptying (GE) and intestinal transit (IT) in mice with different treatment protocols. Mice were randomly allocated to 5 groups (n = 15/group) according to their treatment protocols (control, administered with antikinetics: atropine, dopamine, or with pro-kinetics: itoride, bethanechol) for each GE and IT test. Each group was subsequently divided into 3 subgroups (n = 5) pre-treated with different PD doses (0, 2.5, and 5 mg/kg). Pre-treatment with PD in the control treatment group of mice showed reduced GE and IT in a dose-dependent manner. At the maximum PD effect, GE and IT were reduced by 63% and 50%, respectively, compared with those in the normal control group. In the groups given atropine or dopamine, pre-treatment with PD further reduced GE and IT by 35% to 58%, respectively. The PD pre-treatment dramatically reduced the GI motility enhanced by itopride and bethanechol. On the whole, these results suggest that PD treatment might be beneficial in motility-induced diarrhea.

14

The influence of selenium on antifungal activity of extracts of Codonopsis pilosula hairy roots

63%

Janas J., Bogusz D., Wielanek M., Sklodowska M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

15

Biological activity of saponins from alfalfa tops and roots against Colorado potato beetle larvae

63%

Szczepanik M., Krystkowiak K., Jurzysta M., Bialy Z.

Acta Agrobotanica

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2001

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tom 54

|

nr 2

The total saponins of alfalfa, Medicago sativa L., included in the diet of Colorado potato beetle larvae reduced their feeding, growth rate and survival. The biological activity of those compounds coming both from the roots and from the aerial parts is ,y correlated with the dose. Larvae reared on leaves treated with a 0.5% dose virtually did not feed at all and died after 4-6 days. Lower saponin doses (0.0 I and %) reduced the insects - feeding to a lesser degree. However, they inhibited their ,caused an extension of the larval stage and mortality at a level of76.7- 100%. No major differences have been found in saponin activity depending on its localization in the plant.

16

A novel approach for assessment of biopesticides effect on aphids

63%

Leszczynski B., Urbanska A., Lukasik I., Krzyzanowski R., Matok H.

Pestycydy

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2004

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nr 3-4

17

Biological activity of secondary plant substances glucosinolates, alkaloids and saponins, expressed by their effects on development of Colorado potato beetle, Leptinotarsa decemlineata Say

63%

Waligora D.

Journal of Plant Protection Research

|

1998

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tom 38

|

nr 2

Biological activity of secondary plant substances: glucosinolates, alkaloids and saponins in relation to Colorado potato beetle, Leptinotarsa decemlineata was studied in detail. Larvae and adult insects were reared on potato leaves sprayed with tested compounds applied in three different concentrations. The insect behaviour, mortality and weight changes were observed. Among examined chemicals saponins proved to be the most active against Colorado potato beetle. They showed antifeedant properties and caused high larval mortality.

18

Antifungal activity of saponins originated from Medicago hybrida against some ornamental plant pathogens

63%

Saniewska A., Jarecka A., Bialy Z., Jurzysta M.

Acta Agrobotanica

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2006

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tom 59

|

nr 2

Antifungal activity of total saponins originated from roots of Medicago hybrida (Pourret) Trautv. were evaluated in vitro against six pathogenic fungi and eight individual major saponin glycosides were tested against one of the most susceptible fungi. The total saponins showed fungitoxic effect at all investigated concentrations (0.01%, 0.05% and 0.1%) but their potency was different for individual fungi. The highest saponin concentration (0.1%) was the most effective and the inhibition of Fusarium oxysporum f. sp. callistephi, Botrytis cinerea, Botrytis tulipae, Phoma narcissi, Fusarium oxysporum f. sp. narcissi was 84.4%, 69.9%, 68.6%, 57.2%, 55.0%, respectively. While Fusarium oxysporum Schlecht., a pathogen of Muscari armeniacum, was inhibited by 9.5% only. Eight major saponin glycosides isolated from the total saponins of M. hybrida roots were tested against the mycelium growth of Botrytis tulipae. The mycelium growth of the pathogen was greatly inhibited by hederagenin 3-O-β -D-glucopyranoside and medicagenic acid 3-O-β -D-glucopyranoside. Medicagenic acid 3-O-β -D-glucuronopyranosyl-28-O-β -D-glucopyranoside and oleanolic acid 3-O-[β -D-glucuronopyranosyl(1→2)-α -L-galactopyranosyl]-28-O-β -D-glucopyranoside showed low fungitoxic activity. Medicagenic acid 3-O-β-D-glucopyranosyl- 28-O-β -D-glucopyranoside, hederagenin 3-O-[α -L-rhamnopyranosyl(1→-2)-D-glucopyranosyl( 1→2)-D-glucopyranosyl]- 28-O-β -D-glucopyranoside and hederagenin 3-O-α -D-glucuronopyranosyl-28-O-β -D-glucopyranoside did not limit or only slightly inhibited growth of the tested pathogen. While 2β, 3β-dihydroxyolean-12 ene-23-al-28-oic acid 3-O-β -D-glucuronopyranosyl-28-O-β -D-glucopyranoside slightly stimulated mycelium growth of B. tulipae.

19

Effect of saponins on the intake of water and feeds by broiler chickens and as their body weight gains

63%

Podgorski W., Majewski T.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

|

2002

|

tom 20

20

Ocena mozliwosci wykorzystania saponin lucerny [Medicago ssp.] do zwalczania mszycy burakowej w uprawie bobiku

51%

Ksiezak J., Bialy Z., Kot I.

Progress in Plant Protection

|

2008

|

tom 48

|

nr 3

903-907

Ograniczanie wyników

Chromatographic analysis of rapeseed glucoside fractions

Simplification of a haemolytic micromethod for toxic saponin quantification in alfalfa

Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiflorum A.DC.

Analysis of gypsogenin saponins in homeopathic tinctures

Metoda oznaczania saponin w niektórych środkach spożywczych

The ability of Candida maltosa for hydrocarbon and emulsified hydrocarbon degradation

The effect of alfalfa [Medicago sativa] saponins on Botrytis tulipae and Phoma narcissi growth

The influence of alfalfa saponins and mustard glucosinolates on the fertility of Colorado potato beetles [Leptinotarsa decemlineata Say]

Effect of alfalfa [Medicago sativa L.] saponins on development and fertility of grain weevil [Sitophilus granarius L.]