In this study, five halotolerant Bacillus isolates from Aran-Bidgol Saline Lake in Iran were identified from saline environments. Screening of the bacteria led to the identification of a unique halo-thermotolerant Bacillus. On the basis of genetic and phenotypic data, this isolate was closely related to Bacillus licheniformis. But isolated Bacillus can be distinguished from B. licheniformis by salt tolerance, 16SrDNA sequence and some different physicochemical properties. Thus, suggested that the isolate was not the known Bacillus. Optical density analysis indicated strong biofilm formation for this strain. Also this isolate exhibited average tolerance to 1-25 mM concentrations of zinc and was sensitive to all concentrations of nickel. In biosurfactant production assay, this Bacillus exhibited the high activity for semi-quantitative oil displacement test (3.14 ±0.02 cm²) and evaluated positive for drop-collapse test and hemolytic activity. Moreover, amylase, protease and DNase enzymes produced in presence of 10-20% salt of medium. Therefore, identified Bacillus could supply potential microbial materials for bioremediation purposes and biotechnological applications.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.