Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 15

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  rzesistek pochwowy
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Wrażliwość Trichomonas vaginalis na wysychanie

100%
Twelve strains of T. vaginalis were tested for their sensitivity to dessication at room temperature. Each of them, in the quantity of 0.5 cm³ of 36 hrs old culture (containing 2.5 mln cells) was placed in 5 sterilized tubes containing 2:5 cm³ of: sterilized Roiron medium, urine, 0.9% NaCl, tap water and distilled water. After shaking, the suspension of Trichomonas of each tube in the quantity of 0.15 cm³ was placed on depression slides. The slides were then placed in sterilized, not tightly covered Petri dishes, which were kept at room temperature. The suspension of 3 slides was examined under microscope and inoculated on Roiron medium prior to complete dessication. The same procedure was followed with respect to the fourth slide but only after the suspension had, dried. The suspension remaining in the tube (2.4 cm³) served as control. The experiment showed that in a drop of 0.9% NaCl, urine and Roiron medium, Trichomonas remains active for 120 min. When transferred to Roiron medium within that time, Trichomonas reproduces in a normal way which was not observed after a transfer from the suspension dried up. In a drop of water Trichomonas loses its ability to reproduce within a time shorter than 120 min. The results speak in favour of a possibility of a non-sexual infection with Trichomonas.
Using of fresh slides and culture T. vaginalis was found in 1094 persons (3.20%). It has been seen the morhpological differentiation of T. vaginalis connected with the age, physiological state of macroorganisms and the clinic picture of trichomoniasis. In vivo, the spheroidal as well as ameboidal forms of T. vaginalis were observed. The latter ones, characteristic in acute trichomoniasis, were often seen in pregnant women. On the other hand, nonmobile round-shaped forms of T. vaginalis occurred in vaginal contents of girls, women in child-bed and old (5 years after menopause) women. It seems, that morphologicaly variability of T. vaginalis depends on changes of specific environment of human vagina, which is very sensitive to hormon responses. It may be considered as adaptation of parasite to different biological conditions of vaginal environment.
Three cases of prostatitis caused by the invasion of T. vaginalis and C. albicans which has been found in different biological materials were described. After per rectum examination perineum biopsy of prostata was performed in all patients; in histopathological preparations painting at the inflammed reaction of that gland the fungi were detected. Also, the same microorganisms were proved in sexual partners of those patients but multifocal invasion of C. albicans - including genital and urinary organs, mouth and alimentary tract - also in members of their family.
Trichomonas vaginalis was incubated with bacteria for 30 min. After centrifugation of Staphylococcus aureus, incubation of T. vaginalis was continued for 40 min., 120 min. and 6 hrs. It was found that bacteria have been intensively phagocytated through whole surface of protozoan. Phagosome with bacteria at different stages of degradation, up to defecation of undigested residues were observed. It was observed that some bacteria, apparently not susceptible to digestion, were immersed directly in cytoplasm (outside vacuoles).
Estimation of virulence of T. vaginalis strains injected subcutaneously to mice was done. T. vaginalis strains were obtained from the urinogenital tracts of patients with different clinical forms of trichomonosis. The virulence of T. vaginalis strains measured by infiltration size was found to depend upon a clinical form of trichomonosis. It was moreover shown that the cutaneous infiltration size is proportional to a dose of Trichomonas administered subcutaneously. Histopathological analysis of cutaneous infiltration itself is not decisive as to the strains virulence, yet in addition to macroscopic examination it may be a criterium in the studies on strain pathogenicity.
It has been shown that glycoprotein and polysaccharide fractions obtained from T. vaginalis cells can be employed as antigens in serodiagnosis of trichomonosis. This refers especially to the polysaccharide fraction because of its easy availability, solubility in aqueous solutions, high diagnostic specificity and sensitivity. It has been stated that the diagnostic value of polysaccharide antigen of T. vaginalis, in occult trichomonosis in men particularly, is higher than the effect of the secretion examination by means of culture, even after the prostatic gland massage.
Swabs of the genito-urinary tract of 638 women were taken and subsequently examined with the use of the latex agglutination test (LAT). The results were compared with wet mounts and cultures. The sensitivity of these methods were: 98, 30.7, and 46.1; the specifity: 99.8, 100, and 100; the usefulness: 99.5, 94.4, and 95.6, respectively. In acute trichomonosis the results of LAT and the conventional methods were almost identical but in the chronic and asymptomatic forms of the disease, the results obtained with the use of LAT were better whereas the conventional methods were of little significance. Any cross-reactivity of specific anti- Trichomonas vaginalis immunoglobulin G was observed neither between the antigens of fungi from the genus Candida nor between bacteria. Quantitative analysis of this new test indicates that the use of the latex reagent prepared in our laboratory makes it possible to detect the soluble T. vaginalis antigens in concentration of 50 ng/ml. Our results indicate that as a very sensitive and specific test, LAT can be useful in the diagnosis of asymptomatic trichomonosis when the microscopic examination and culture are ineffective.
The trichomonacidal activity in vitro of 6 new derivatives of theirs containing amine and halogenaryloalkyl groups in comparison with metronidazole, ornidazole and tinidazole has been examined. For all the examined compounds curves of action were obtained after 30 min. and lethal concentrations for 50% population (CL₅₀) of three strains of T. vaginalis used for experiments were calculated. The trichomonacidal action of the following compounds was especialy strong: 2-/1-methylpiperazin-4-yl/ethyl-4-chlorbenzhydryl ether dihydrochloride and 2-/1-benzylperhydro-1,4-diazepin-4-yl/ethyl 2,4-dichlorbenzyl ether dihydrochloride. The trichomonacidal action of these compounds was several times stronger than that of metronidazole and tinidazole.
The authors showed the frequency of occurrence of trichomonadosis and candidosis in 31 842 women living in the Upper Silesia territory. The most extensive invasion of T. vaginalis and C. albicans was found in autumn, and in women 24-34 years old.
Interaction between T. vaginalis and P. aeruginosa was studied in vitro. Protozoan cells were incubated in 0,9% NaCl at 37°C for 30 and 150 min. with bacteria, alive or killed by heating for 10 min. at 100°C. Transmission electron microscope observations showed weak fagocytic activity (<10%) of T. vaginalis against living P. aeruginosa. Phagocytosis of these bacteria caused death of T. vaginalis. Desintegration and autolysis of T. vaginalis resulted probably from action of exotoxin A produced by P. aeruginosa. Phagocytosis of dead P. aeruginosa has not been observed.
During 5 years cycle a prophylactic gynaecological, cytological and parasitological examinations of 1918 women were performed. 12.8% of women were infected with T. vaginalis. Decreasing percentage of infections was observed: from 9.92% in 1984 to 2.2% in 1988. In the I° of Papanicolaou cervical smears T. vaginalis has been found in 1.60% of cases, in the II°-in 5.2%, in the III°- in 14.29%, and in the IV°-in 22.22% of cases. In women with normal cervix uteri T. vaginalis have been found in 5.01% of cases, and in women with hypertrophical cervix and partially epidermised ectopy with ovula Nabothi in 15.28% of cases.
Data concerning 604 girls up 18 year old were analysed. On the basis clinical symptoms and results of culture of various biological materials on selected media - the monofocal trichomonadosis concerning the most often vulva and vagina, rarely urethra or other regions of urinary organ as well as the multifocal trichomonadosis, particularly complicated by mycosis were proved. It was found the convergence statistically significant (P≤0,5) of the invasion of T. vaginalis with some symptoms of inflammation of genital and urinary organs; the highest values of Pearson-Bravais coefficient was obtained in trichomonadosis of genital organs and vaginal discharges, pain of vulva and vagina, the picture of colpitis maculosa, but in the multifocal one also with itching and pain in urethra and dysuria. T. vaginalis without symptoms occurred very seldom in the girls.
19 024 girls (aged: from 0 to 18) attending the Pediatrie Gynaecology Outpatient Department and the Center for Treatment of Parasitic and Mycotic Diseases were examined. The diagnosis of infection with T. vaginalis was based on preparations using various methods and cultures on specific media, from vaginal content and urine. It was found that the frequency of T. vaginalis invasions in girls had lessened 2-6 times in different groups from 1960 till 1985 and the higher percent of T. vaginalis in girls over 10 years old had been observed than in younger ones. There exists the statistically significant correlation (P < 0,02) between T. vaginalis invasions in girls and other persons from their environment (families).
The 50% trichomonocidal concentration of 7 examined ethers (T. vaginalis strain N° 1/86, Roiron medium, safranine staining, 30 min.) was estimated by means of dose-response curve. The minimal mycostatic concentration (Candida albicans L-45, Geotrichum candidum; 3% Sabouraud agar, 37°C, 24 hr) was calculated with the aid of regression equation. All examined ethers show in vitro a marked trichomonocidal effect comparable with activity of ornidazole or phenol. Mycostatic activity of new compounds is many times higher than activity of phenol, but considerably lower than that of klotrimazole. The strongest complex trichomonoddal and mycostatic effect-comparable to ornidazole or klotrimazole - shows the new derivative N,N-diethylaminoethyl oxime of 1-tioflavone (compound II), which is fairly toxic for mammal.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.