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Effect of activated charcoal on in vitro propagation of Iris x hollandica

100%

Bach A.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 4-6

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In vitro propagation of Centaurea ragusina L., a Croatian endemic species

100%

Pevalek-Kozlina B.

Acta Biologica Cracoviensia. Series Botanica

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1998

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tom 40

A rapid clonal propagation method was developed for Centaurea ragusina L. (Asteraceae), a rare Croatian endemic species. Shoots originated from aseptically germinated seeds were used for culture initiation. The highest multiplication rate, 4.6 shoots per explant, was achieved in a 4-week culture period on half-strength Murashige and Skoog medium supplemented with 1.0 µM 6-benzylaminopurine and 2.9 µM gibberellic acid. Excised shoots were rooted on the same basal medium with 2.5 µM indole-3-butyric acid added. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

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Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants

70%

Hanus-Fajerska E., Wiszniewska A., Czaicki P.

Acta Agrobotanica

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2012

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tom 65

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nr 1

In this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. jasminea, and D. tangutica was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, and 0.65 g dm-3 calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B5 vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in D. tangutica, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to in vivo conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.

Ograniczanie wyników

1 Acta Agrobotanica

1 Acta Biologica Cracoviensia. Series Botanica

1 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Bach A.

1 Czaicki P.

1 Hanus-Fajerska E.

1 Pevalek-Kozlina B.

1 Wiszniewska A.

1 2012

1 1998

1 1988

Effect of activated charcoal on in vitro propagation of Iris x hollandica

In vitro propagation of Centaurea ragusina L., a Croatian endemic species

Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants