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Arbuscular mycorrizal (AM) fungi may enhance plant growth and polyphenol production, however, there have been limited studies on the relationships between root colonization of different fungal species and polyphenol production on cultivated Allium porrum (garden leek). The effects of inoculation of AM fungi spores from Rhizophagus intraradices, Giga -spora margarita, Glomus geosporum, Paraglomus occultum, Claroideoglomus claroideum, and Glomus species on colonization of garden leek roots and symbiotic changes in polyphenol production and plant growth were evaluated in greenhouse experiments. There were significant differences (p < 0.05) in colonization of leek roots by AM fungi species. The greatest level of root colonization was recorded on plants inoculated with R. intraradices (73%) and the lowest level on C. claroideum (3.2%). Significant differences (p < 0.05) in plant height were recorded between AM inoculated plants and the controls. Polyphenol levels differed significantly (p < 0.05) between garden leek plants inoculated with AM fungi and the non-inoculated controls. The percentage increases in polyphenol (a derivative of kaempferol) on garden leeks inoculated with G. geosporum relative to the untreated controls ranged from 28 to 1123%. Due to symbiosis with different AM species, other polyphenols decreased in some instances (negative values) and increased in others for values of up to 590%. Results showed that AM fungi species exhibited remarkable differences in polyphenol levels in garden leeks. The high polyphenol production by garden leek plants inoculated with G. geosporum, and Glomus species could be exploited for enhanced resistance of garden leeks to insects and diseases. This research highlights an understudied area, notably the relationships between AM fungal inoculations, root colonizations and polyphenol production in garden leeks. The findings can be utilized to improve pest resistance and the quality of garden leek plants.
Sandy everlasting, Helichrysum arenarium, a perennial belonging to Asteraceae family, is a source of valuable herbal material and therefore is under partial protection in Poland. It grows in sunny places on sandy soils, in abandoned fields, along roadsides and in dry forests and thickets, where it enters into symbiosis with arbuscular mycorrhizal fungi. The studies on the biology, ecology and cultivation of sandy everlasting have been vigorously pursued since 2003. The aim of this work was assessing the influence of root colonisation by mycorrhizal fungi on the growth and development of H. arenarium cultivated on clayey soil. Two independent experiments (in the pots and in field) were performed. In the first one, under laboratory conditions, the effect of inoculation of soil with arbuscular fungus Glomus intraradices on the biometric features of sandy everlasting seedlings was analyzed. The second experiment was designed to show the association of the root colonisation level with the biometric features of generative shoots grown in an open field. It was demonstrated that sandy clay is an appropriate medium for growing sandy everlasting, enabling a proper development of seedlings and ensuring high inflorescence yield. The amendment of soil with inoculum resulted in a significant decrease in the mass of vegetative and generative shoot parts and made the cultivation more expensive. The inflorescence yield (Inflorescentia Helichrysi) obtained from inoculated plots was lower by 36%.
The experiment was conducted to evaluate the effect of concentration of nutrient solution on root colonization with AMF, growth and flowering of ivy geranium (Pelargonium hederaefolium ‘Ville de Paris’) and garden geranium (Pelargonium hortomm ‘Aquarello’) cultivated on ebb-and-flow benches. Four concentrations of nutrient solution were applied: EC 1.0. 1.5, 2.0 i 3.0 mS‧cm⁻¹. The concentration of nutrient solution did not affect colonization of root system with AMF. The effect of concentration of nutrient solution and mycorrhization on fresh matter, shoot length and shoot number of ivy geranium was negligible. The earliest flowering was noted in plants fertilized at 3.0 mS‧cm⁻¹. These plants had also the greatest number of inflorescences. Garden geranium grown at mS‧cm⁻¹ obtained the highest fresh matter and the longest shoots. Mycorrhizal plants had lower fresh matter and shorter shoots than the non-mycorrhizal ones. Neither the concentration of nutrient solution nor mycorrhization did affect the flowering of garden geranium.
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