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Phenolic acids from leaves and roots ofJovibarba sobolifera (Sims.) Opiz were analysed by means of the two-dimensional thin layer chromatography and by the RP-HPLC method. Twelve phenolic acids were identified by 2D-TLC: chlorogenic, gallic, protocatechuic, caffeic, p-coumaric, p-hydroxy- benzoic, p-hydroxyphenylacetic, vanillic, syringic, ferulic, synapic and a-rezorcylic. The RP-HPLC enabled describing the contents often major phenolic acids in the examined material.
The aim of our work was to investigate the antioxidant activity of Rheum palmatum extracts. Antiradical activity against DPPH and ABTS radicals, reducing power FRAP and total phenolic contents, were investigated in one-, two- and three-year-old roots of rhubarb fertilized with nitrogen at the rates of 50, 100 and 200 kg N/ha. It was proved that nitrogen dose as well as the age of plantation did significantly influence antioxidant activity and total phenolic of root extracts. The highest values were determined in one-year-old plants, antioxidant activity ranged the level of 112–203 µM Trolox/g and total phenolic compounds average content was 22 mg GAE/g FW. Two-year-old roots were characterized by 3–11%, and three-year-old ones by 15–23% lower antioxidant activity and phenolic compounds content. When nitrogen dose increased, polyphenols content, DPPH and FRAP values increased as well, although, ABTS showed a different tendency.
A concentration of two phenylethanoids in the roots of two species: Rhodiola kirilowii and rosea were compared, aqueous and hydroalcoholic extracts from those plants were also analyzed. In order to determine the content of p-tyrosol and salidroside, the ultra performance liquid chromatography connected with a tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The obtained results shown that content of measured phenylethanoids depends on Rhodiola species. Roots of R. kirilowii contain more p-tyrosol, while R. rosea roots are reacher in salidroside. Our results indicate that the application of UPLC MS/MS method allows to determine the phenylethanoids content in tested samples with satisfactory precision.
The processes of growth and development as well as the yield quality of crops depend on the abundance of soil nutrients and the ability of the plants to uptake nutrients. Nutrients can be taken up more efficiently after application of a biostimulant. The effect of biostimulants depends on the content of their active substance as well as the dose, timing and frequency of their application. In 2009-2011, a controlled field experiment was conducted in the Kuyavian-Pomeranian Province (53°13′N; 17°51′E). The objective was to analyse the effect of the timing and doses of foliar application of the biostimulants: Kelpak SL (seaweed extract containing phytohormones) and Asahi SL (a mixture of phenolic compounds) on the content of macroelements: Mg, P, Ca, N, Na and K in the storage roots of carrot directly after harvest and after storage (for 6 months in chambers with controlled conditions: temp. +1°C, Rh 95%). Kelpak SL was applied once in a dose of 3 or 2 dm3 ha-1, twice in doses of 3+2 dm3 ha-1 or 2+2 dm3 ha-1 at intervals of two or four weeks, as well as three times in doses 3+2+2 dm3 ha-1 or 2+2+2 dm3 ha-1, every two weeks. Asahi SL was applied twice in doses 0.5+0.5 dm3 ha-1,at a two-week interval. The first application of biostimulants was always performed at the 4-leaf stage. The study showed that the biostimulants Kelpak SL and Asahi SL, irrespective of the dose and frequency of application, increased the N concentration in the carrot roots. An increase in the Mg, P, Na and K concentrations was observed after a single application of Kelpak SL in a dose of 2 dm3 ha-1, and in the Ca concentration after a dose of 3 dm3 ha-1. Asahi SL did not affect the Mg, P, Ca and Na concentrations but increased the K content in the roots. After storage, the content of Mg, Na and K decreased, whereas the concentration of P, Ca and N did not change. Directly after harvest and after storage, positive correlation between N and K and between N and Na, as well as between Na and K was indicated.
A comparative study of the sterol components of roots and herb of H. pilosella was carried out using GCMS techniques, β-sitosterol, stigmasterol, campesterol and α-saccharostenon were identified in both fractions. Stigmast-7-en-3-ol was identified only in herb and stigmast-4-en-3-on only in roots. Sitosterol and stigmasterol were the predominant sterol components in analysed raw materials. Besides, by means of the weight method the total amounts of sterols was determined: in herb 0.24% and in roots 0.16%.
Nitrate uptake in right-side out plasma membrane vesicles isolated from cucumber roots was characterized. Nitrate uptake into vesicles was driven by an artificially imposed pH gradient. The uptake was strongly inhibited by phenylglyoxal, an arginyl residue modificator. Only a slight repression of NO⁻₃ transport in vesicles was observed in the presence of NEM, a thiol group reagent. pCMBS, an other thiol reagent and DEPC, an effector of histidine residue, had no effect on the nitrate transport in plasma membranes. ATP-driven proton transport in vesicles was not significantly affected in the presence of both, phenylglyoxal and DEPC, whereas pCMBS and NEM abolished it almost completely. The possible role of the particular amino acids residues in the active nitrate transport is discussed. NO⁻₃ uptake into vesicles isolated from both, nitrate-induced and nitrate-depleted plant material was higher than that observed in the vesicles obtained from uninduced plants. Thus, isolated vesicles reflect the well-known in vivo response of intact plants on the exogenous nitrogen regime.
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