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1
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Differentiation of European brown hare syndrome and rabbit haemorrhagic disease viruses by amplification and restriction analysis of capsid protein gene fragment

100%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
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2000
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tom 44
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nr 2
The aim of the studies was differentiation of EBHS and RHD viruses by amplification of the fragment coding the first part of the capsid protein (VP60) and digestion of this fragment by restriction endonucleases. The fragments of 4 strains of EBHS (two Polish isolated in 1992 and 1998; two standard Italian and French) and 5 strains of RHD (Polish strains isolated in 1988, 1994, 1997 and 1998) viruses were amplified by RT-PCR with the use of primers selected for each virus. The specific fragments of 726 bp of EBHS and 734 bp of RHD viruses were demonstrated after amplification with the use of primers selected for each virus. Negative results were obtained when primers selected for the RHD virus were used to amplify the fragment of EBHS virus and on the contrary. The hybridisation by the Southern blot method revealed the cross reaction of DIG-Iabelled probes prepared for each virus. The endonucleases BamH I and Pst I were used in the restriction analysis of fragments. The 734 bp fragments of all strains of RHD virus digested by BamH I and Pst I enzymes demonstrated the same restriction profiles. The 726 bp fragments of strains of EBHS virus were not digested by Pst I, whereas in two strains (standard Italian and Polish isolated in 1988) after digestion with BamH I different restriction profiles in comparison with RHD virus were demonstrated.
2

Cassava chloroplast DNA: Isolation, comparisons and cloning

86%
Yeoh H-H., Joseph S. S.
Acta Physiologiae Plantarum
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1995
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tom 17
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nr 4
3
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Restriction analysis of capsid protein gene fragment of two Polish strains of European brown hare syndrome virus

72%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
|
2000
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tom 44
|
nr 2
Polish strains of EBHS virus isolated in 1992 (NP11/92) and in 1988 (L/98) were compared with standard Italian and French strains by restriction analysis of fragment coding the first part of capsid protein (VP60). The 726 bp fragment of capsid protein gene of strains tested was obtained by RT-PCR. The digestion of 726 bp fragments by BamH I endonuclease, revealed the same restriction profile for strain L/98 as Italian standard strain. These fragments of strain NP11/92 and French standard strain were not digested by BamH I. Endonuclease Sma I did not digested the amplified fragment of strains tested. The 726 bp fragment of strain L/98 demonstrated different restriction profiles for BsuR I and Hpa II enzymes in comparison with strain NP11/92 and standard strains. These results revealed differences in restriction profiles of fragment coding the first part of VP60 of Polish strains in comparison with standard strains and also between two Polish strains isolated in 1992 and 1998. These differences suggest the possibility of genotype changes of Polish EBHS strains isolated in different years.
4

Restriction analysis of mtDNA in whitefish [Coregonus lavaretus] from Swaderki hatchery

72%
Brzuzan P., Jagodzinski G.
Natural Sciences
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1998
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tom 01
5

Genetic variability of four natural isolates of the Stilpnotia salicis multiple-enveloped nuclear polyhedrosis virus

72%
Strokovskaya L., Ziemnicka J., Michalik J.
Acta Biochimica Polonica
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1996
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tom 43
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nr 4
Viral genome DNA from four different multiple-enveloped nuclear polyhedrosis virus isolates, obtained from naturally infected larvae of satin moth (Stilpnotia salicis), a pest of poplar tree (Populus) was analysed. Larvae were collected over a period of 11 years, from 1978 to 1989. The genomic DNA restriction patterns pointed to heterogeneity of these wild-type viruses. The differences observed in isolates of several years revealed limited restriction fragment length polymorphism and showed that these viruses contained distinct, but closely related genotypes. The genome size of SsMNPV was established as 128-134 kb, based on /fmdIII and Sacl restriction analysis.
6

Restriction analysis of genetic variability of Polish isolates of tomato black ring virus

72%
Jonczyk M., Borodynko N., Pospieszny H.
Acta Biochimica Polonica
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2004
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tom 51
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nr 3
Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV iso­lates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction en­zymes. On the basis of the restriction patterns, the variable and the conserved re­gions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.
7

The detection of food components capable of preventing DNA oxidative damage by restriction analysis

58%
Kolodziejski D., Brillowska-Dabrowska A., Bartoszek A.
Polish Journal of Food and Nutrition Sciences
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2011
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tom 61
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nr Suppl.1
8

Characteristics of genes encoding structural protein of Polish strains of goose parvovirus

58%
Wozniakowski G., Samorek-Salamonowicz E., Kozdrun W.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
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nr 2
119-122
The aim of this study was to come up with molecular characteristics of Polish strains of goose parvovirus (GPV) on the basis of genes encoding their structural proteins. Ten field GPV strains and two vaccine strains: B-38 and MFP were used. The PCR- RFLP method based on three distinct endonucleases: HinfI, MboI, and RsaI, which had restriction sites within the sequences of examined genes, was applied. It was found that the homology of VP1, VP2, and VP3 among the studied strains ranged from 50% to 100%. The major differences in restriction patterns were found after application of HinfI, whereas 100% homology was observed after Rsal digestion. Significant differences were observed in restriction profiles of vaccine GPV strains. The study revealed that the application of PCR-RFLP for the analysis of VP1, VP2, and VP3 genes allowed for their molecular characteristics and differentiation between the vaccine and field strains.
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