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The measurement of respiration activity of microorganisms in particular aggregate fractions for 3 soils was performed. And an attempt was made to determine the relation between the quantity of bacteria and fungi. The correlation between the size of aggregates and respiration activity of microorganisms was found out.
The authors investigated the impact of different sewage sludge doses on the developmental dynamics of soil microorganisms as well as on soil respiration activity. The following soil combinations were employed: control (soil + NPK), 2 t d.m. of sewag sludge·ha⁻¹·year⁻¹ + NPK, 4 t d.m. sewage sludge·ha⁻¹·year⁻¹ + NPK and 8 t d.m. sewage sludge·ha⁻¹·year⁻¹ + NPK. The experiments were carried out in field conditions. During the first year of analyses, winter rye was used as the test plant, while in the second year rye was replaced by potatoes as test plants. Soil samples for analyses were collected during the consecutive plant developmental stages and, using the plate method, the total numbers of bacteria, fungi as well as pathogenic bacteria from the Salmonella, Clostridium perfringens and Escherichia coli genera were determined. In addition, using the absorption method, the amount of CO₂ released from the soil was also ascertained. On the basis of the obtained results it was found that the introduction into the soil of organic matter in the form of sewage sludge failed to cause any statistically significant changes in the total counts of bacteria and fungi. Moreover, pathogenic bacteria were found in the examined soils (Salmonella sp., Clostridium perfringens and Escherichia coli) and they persisted in the soils even after 66 (Salmonella sp.) and 94 days (Clostridium perfringens and Escherichia coli) of analyses. The analysis of the CO₂ released on the control plot and on other soil treatments showed that the fertilization of soils with sewage sludge resulted in a slight increase of their respiration activities in relation to the control.
The range of solar radiation reaching the air-water inter-phase, medium wave UV radiation, i.e. UVB 290–320 nm and UVA 320–400 nm, is of the highest biological importance due to its harmful effects. Radiation within this range causes DNA damage (lethal effect) or limits the growth of organisms by inhibiting enzyme synthesis, reducing active transport, or by inducing mutations. The studies were carried out in 2007 based on samples water collected from surface microlayer (SM) (up to 150 μm) and subsurface water (SSW) (25 cm) of pelagic zone of eutrophic lake. The representative collection of bacterial strains was isolated from collected samples. The following are measured: the DNA and cellular protein synthesis activity, respiration activity of the bacteria and activity of hydrolytic enzymes in control cultures, subjected to UVB radiation (applied dose 100 mW cm⁻²) and with and without humic substances (HS) (final concentration 100 mg L⁻¹) playing role of compounds potentially protective from UV radiation. UVB irradiation had the strongest inhibiting impact on production of DNA in bacterial cells (12–23% of that in non-irradiated samples). UVB radiation also inhibits the synthesis of cellular protein (27–43% of that in non-irradiated samples) and bacterial respiration activity (44–48%). UVB radiation had by far the lowest impact on the activity of hydrolytic enzymes. HS may function as a protective agent against UV radiation only in DNA synthesis. No significant differences in response to UVB were found between planktonic and neustonic bacteria.
Procesy wzrostu i rozwoju roślin są regulowane przez hormony roślinne jak również przez czynniki środowiskowe np. temperaturę. Celem badań było wyznaczenie temperaturowej zależności aktywności oddychania komórek segmentów koleoptyli Zea mays L., indukowanego przez IAA i 4-Cl-IAA. Badania przeprowadzono na 10 mm, etiolowanych segmentach koleoptyli kukurydzy z których usunięto pierwszy liść. Pomiary zawartości tlenu w środowisku segmentów koleoptyli kukurydzy przy braku i w obecności wybranych substancji wzrostowych przeprowadzono przy użyciu elektrody tlenowej oraz tlenomierza. Wykazano, że pobieranie tlenu przez segmenty koleoptyli kukurydzy jest zależne od temperatury.
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