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In the recent paper genotoxic effects of daminozide and its metabolites were tested. Evaluation of the mutagenic effect of daminozide was by: (i) the Salmonella/mammalian microsome Ames test with S.typhimurium TA97, TA98, TA100 and TA102. (ii) E. coli PQ37 strain to reveal an induction of the SOS response, (iii) S. typhimurium TA1538 (uvrB) and TA1978 (uvr+) to detect the chemicals bound covalently to DNA (repair test). Daminozide was not mutagenic in any of the S.typhimurium strains and did not induce damages in DNA recognized by correndonuclease II, as shown by the repair test. Only metabolites of daminozide induced the SOS system.
Background. Considering the fact that pesticides are commonly used in agriculture, continuous monitoring of these substances in food products is of great significance. Residues of these substances can be present in crops after harvest. Objective. The aim of this study was to evaluate presence of pesticide residues in fruiting vegetables from production farms in south-eastern region of Poland in 2012–2015. Material and methods. 138 samples were tested using accredited test methods. The monitoring programme covered determination of 242 pesticides. The tests covered tomato, cucumber and pepper crops. The test results were interpreted in accordance with criteria included in the European Commission recommendations published in the document SANCO/12571/2013 (now superseded by Document SANTE 2015), as well as on a basis of the maximum residue levels in force in the EU Member States. Results. Pesticide residues were found in 47 samples, representing 34% of all tested samples. 17 active substances were found, belonging to fungicides and insecticides. Azoxystrobin (38%), boscalid (28%) and chlorothalonil (21%) were most commonly found in fruiting vegetables testing samples. Non-compliances related to use of plant protection product not authorized for protection of a given crop were observed in 6% of analysed samples. However, pesticide residues of fruiting vegetables in quantities that exceed the maximum residue levels (NDP, ang. MRLs), as well as substances which use for plant protection is forbidden were no found. Conclusions. Crops monitoring is used to determine to what extent such products are contaminated with pesticide residues, and ensures protection of customer health.
A field trial was conducted to evaluate the residues of Bispyribac sodium 10 % SC on rice crop during Kharif season 2013 at Kandikai in Thiruvallur District, Tamilnadu, India. Randomized block design was followed withthree treatments forthree replicates. Bispyribac sodium 10 % SC 200 g a.i./ha, 500 g a.i./ha and control (water spray) was sprayed using hand operated Maax battery sprayer with a spray volume of 300 litres per hectare at 15 days after transplantation of rice crop (ADT 45). At harvest, samples of grain, straw and soil were collected replicate wise from each treatment along with the control. These samples were stored in icebox and transfer to the laboratory under cooled condition for analysis.All the residues samples were analyzed for Bispyribac sodium content by a validated HPLC method at the minimum detectable concentration of 0.01 ppm. The result revealed that no detectable level of Bispyribac sodium in straw, grain and soil at harvest. Thepost treatmentand pre-harvest intervalwas 60 days after transplantation.
The paper presents some aspects of relations between modern plant protection of apple orchards and ecotoxicology. Based on significant similarity of the shape and size of apple and tomato fruits the relation between the doses of active ingredients of plant protection products and their residue levels immediately after treatment was derived for the apples and then quality indices and the risks for human health were estimated. It was found that many of the currently in force MRLs need to be revised and the exposure of consumer should not exceed the acceptable daily intakes (ADIs) and acute reference doses (ARfDs) for pesticide residues.
The aim of present investigation was to evaluate the influence of lowrates application on herbicide residues in soil and roots of sugar beet. Chemical weed control in sugar beet was applied at recommended and reduced (about 50 and 66%) doses of herbicides including substances such as: phenmedipham, desmedipham, ethofumesate, metamitron, triflusulfuron and oil adjuvant applied 4 times at 7- to 10-day intervals starting at the onset of weed emergence. Samples of soil and roots of sugar beet were taken at the day of lifting. Phenmedipham, desmedipham and ethofumesate residues were analysed using HPLC with UV-detection. Metamitron residues were analysed using GLC with ECD detection. At lifting time, in soil samples, where recommended herbicide doses were applied, the residues of separate active substance of herbicides amounted from 0.0056 to 0.0124 mg.kg-1. Sum of all detected residues of applied substances amounted 0.0323-0.0373 mg.kg-1. In sugar beet root samples, the residues amounted respectively 0.0011 to 0.0085 mg.kg-1and 0.0085-0.0224 mg.kg-1. Application of the reduced doses by about 50% influenced on a significant decrease of residues about 38-50% average. For samples, where reduction of herbicide dose amounted 66%, the decrease of residues level, in comparison with results obtained at recommended doses, was statistically significant and amounted 69-77% average. Residues of active substances determined in roots of sugar beet did not exceed EU acceptable limits.
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