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  1. 7 Acta Biochimica Polonica

  2. 5 Archivum Immunologiae et Therapiae Experimentalis

  3. 3 Bulletin of the Veterinary Institute in Puławy

  4. 2 Polish Journal of Veterinary Sciences

  5. 1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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  1. 3 Wegrzyn G.

  2. 2 Herman A.

  3. 2 Kazun B.

  4. 2 Morand M.

  5. 2 Pozet F.

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  1. 1 2015

  2. 1 2013

  3. 2 2011

  4. 1 2010

  5. 1 2006

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1

Law and order in the nucleus: dynamics of replication factories in living cells

100%
Cardoso M. C., Rahn H. P., Sporbert A., Leonhardt H.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 2
2

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

88%
Paprocka G., Kesy A.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 4
A swine vesicular disease virus (SVDV) replication assay in IB-RS-2, SK-6, and PK-15 cell cultures was performed using the xCELLigence system. The cell status was monitored by impedance measurement, expressed as cell index (CI). Proliferation of particular cells was examined at the beginning of the study. The cells exhibited the ability to form a monolayer, and the CI values increased with the cell culture growth. After about 23 h and while still in the growth phase, the cells were infected with decimal virus dilutions (10⁻¹-10⁻⁶) containing from 100 000 to 1 median tissue culture infectious doses (TCID₅₀). SVDV replication in cell cultures induced a change in cell index; together with the occurrence of cytopathic effect (CPE), the CI values declined. A significant correlation between the concentration of the virus used and CPE occurrence was found. The results also enabled determination of cell sensitivity to SVDV infection. The highest sensitivity was exhibited by IB-RS-2, followed by SK-6. To conclude, the xCELLigence System was used effectively and evaluated as being an efficient tool for CPE detection and SVDV replication analysis in cell cultures. Compared to the standard method, it enabled a more precise assessment of viral replication based on the quantitative CI measurement, providing additional current information.
3

Streptomyces and Escherichia coli, model organisms for the analysis of the initiation of bacterial chromosome replication

88%
Messer W., Zakrzewska-Czerwinska J.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
|
tom 50
|
nr 6
4

Effect of coliphage lambda P gene mutations on the stability of the lambda O protein, the initiator of lambda DNA replication

75%
Pawlowicz A., Wegrzyn G., Taylor K.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 1
5

Combined effect of strigent or relaxed response, temperature and rom function on the replication of pUC plasmids in Escherichia coli

75%
Herman A., Wegrzyn A., Wegrzyn G.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 2
6

Prenatal detection of maternal UPD15 in new case with i[15p] by Timing Replication Test [TRT] and methylation analysis

75%
Constantinou M., Kaluzewski B., Helszer Z., Zajac E., Nowacka J.
Journal of Applied Genetics
|
2003
|
tom 44
|
nr 2
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The expression of UVR3 and two putative photolyases, PHR2 and At4g25290, is regulated by light

63%
Sztatelman O., Labuz J., Banas A. K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 3
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture

63%
Cymerys J., Dzieciatkowski T., Slonska A., Bierla J., Tucholska A., Chmielewska A., Golke A., Banbura M. W.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 4
Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
9

In vitro effect of methisoprinol on salmonid rhabdoviruses replication

63%
Siwicki A. K., Pozet F., Morand M., Kazun B., Trapkowska S.
Bulletin of the Veterinary Institute in Puławy
|
2002
|
tom 46
|
nr 1
10

Escherichia coli initiator protein DnaA, cell-cycle and control of lambda plasmid replication

63%
Taylor K., Wegrzyn G., Wegrzyn A., Szalewska-Palasz A., Herman A., Obuchowski M., Srutkowska S., Konopa G.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1996
|
tom 44
|
nr 3-4
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Kinetics of replication of equine influenza viruses in chicken embryos and selected cell lines

63%
Rozek W., Skulmowska-Kryszkowska D., Zmudzinski J. F.
Bulletin of the Veterinary Institute in Puławy
|
1998
|
tom 42
|
nr 1
The kinetics of replication of equine influenza A1 and A2 viruses in different systems e.g. chicken embryos, Vero, MDCK and MDBK cell lines was evaluated. The strains A/Equi/1/Praque 56 and A/Equi/2/Kentucky 81 were used. A dose of 0.2 ml of influenza A1 and A2 viruses with the haemagglutinin titre (HA) 256 diluted to 10⁻³ and 10⁻⁴ respectively, was found out to be optimal for inoculation of chicken embryos. The highest HA titre for viruses A1 and A2 were noticed after a 72 hours of incubation post inoculation. The ID₅₀ and LD₅₀ titres differed by more than 2 log in case of A1 virus. Both equine influenza viruses replicated in Vero, MDCK and MDBK cells; however, MDCK cells have revealed the highest sensitivity to infection. Low haemagglutinin titres found in the medium from infected cell lines have suggested that this is rather poor source of virus antigens for vaccine production.
12

Immunoglobulins anti-endonuclease 32 kDa from Cucurbita pepo var.patissonina affect process of DNA synthesis

63%
Rzepecki R., Szopa J.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 jig IgG per 1 x 106 cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Preincubation of cell nuclei of White bush with IgG generates longer DNA fragments than in controls. Involvement of the 32 kDa endonuclease or an endonuclease-65 kDa protein complex from the nuclear matrix in replication or structural organisation of replication is considered.
13

CD8plus T cell suppressor factors and the control of infection, replication and transcription of human immunodeficiency virus

63%
Copeland K. F. T.
Archivum Immunologiae et Therapiae Experimentalis
|
2001
|
tom 49
|
nr 1
14

An enigma: the role of viral RNA aminoacylation

63%
Haenni A. L., Chapeville F.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 4
The first demonstration on the aminoacylation capacity of the RNA genome of a plant virus appeared more than 25 years ago. Shortly thereafter, aminoacylation of the RNA genome of a number of other plant viruses was observed. This led to considerable work on the tRNA-like region of these viral RNAs, and to the first demonstration of the presence of pseudoknots in their folding pattern. In spite of the vast amount of efforts put into trying to understand the reason for the aminoacylation capacity of certain viral RNA genomes, as yet no clear general conclusion emerges. It rather looks as though the reason for aminoacylation may be different for different viruses, and that aminoacylation may operate at different levels in the virus life cycle. Given that certain RNA viruses possess structures which resemble that of tRNAs at their 5'- or 3'-termini, it is most likely that convergent evolution may have dominated the appearance of such structures in the virus world.
15

Beclin 1 is involved in regulation of apoptosis and autophagy during replication of ectromelia virus in permissive L929 cells

51%
Martyniszyn L., Szulc L., Boratynska A., Niemialtowski M. G.
Archivum Immunologiae et Therapiae Experimentalis
|
2011
|
tom 59
|
nr 6
16

Stem cells and skin regeneration

51%
Staniszewska M., Sluczanowska-Glabowska S., Drukala J.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 3
17

Sequence-specific p53 gene damage by chloroacetaldehyde and its repair kinetics in Escherichia coli

51%
Kowalczyk P., Ciesla J. M., Saparbaev M., Laval J., Tudek B.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr 2
Oxidative stress and certain environmental carcinogens, e.g. vinyl chloride and its metabolite chloroacetaldehyde (CAA), introduce promutagenic exocyclic adducts into DNA, among them 1,N6-ethenoadenine (εA), 3,N4 -ethenocytosine (εC) and N2,3-ethenoguanine (εG). We studied sequence-specific interaction of the vinyl-chloride metabolite CAA with human p53 gene exons 5-8, using DNA Polymerase Fingerprint Analysis (DPFA), and identified sites of the highest sensitivity. CAA-induced DNA damage was more extensive in p53 regions which revealed secondary structure perturbations, and were localized in regions of mutation hot-spots. These perturbations inhibited DNA synthesis on undamaged template. We also studied the repair kinetics of CAA-induced DNA lesions in E. coli at nucleotide resolution level. A plasmid bearing full length cDNA of human p53 gene was modified in vitro with 360 mM CAA and transformed into E. coli DH5α strain, in which the adaptive response system had been induced by MMS treatment before the cells were made competent. Following transformation, plasmids were re-isolated from transformed cultures 35, 40, 50 min and 1-24 h after transformation, and further subjected to LM-PCR, using ANPG, MUG and Fpg glycosylases to identify the sites of DNA damage. In adaptive response-induced E. coli cells the majority of DNA lesions recognized by ANPG glycosylase were removed from plasmid DNA within 35 min, while MUG glycosylase excised base modifications only within 50 min, both in a sequence-dependent manner. In non-adapted cells resolution of plasmid topological forms was perturbed, suggesting inhibition of one or more bacterial topoisomerases by unrepaired ε-adducts. We also observed delayed consequences of DNA modification with CAA, manifesting as secondary DNA breaks, which appeared 3 h after transformation of damaged DNA into E. coli, and were repaired after 24 h.
18

Influence of methisoprinol on the replication of rhabdoviruses isolated from carp [Cyprinus carpio] and catfish [Ictalurus melas]: in vitro study

51%
Siwicki A. K., Pozet F., Morand M., Kazun B., Trapkowska S., Malaczewska J.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 1
Rhabdoviruses constitute one of the most pathogenic viruses isolated from: rainbow trout and carp culture. Several viruses were also isolated from other species of fish. These viruses are mostly "associated with epizootics and heavy losses. Spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV) have been the most extensively studied, due to their significant economic impact. Significant progress has been made towards controlling the major bacterial fish diseases using vaccines, but this approach has not yet been successful in preventing viral diseases in fish culture. However, for an effective therapeutic approach, specific drugs should be developed to selectively inhibit virus replication and/or stimulate antiviral protection. In this investigation we examined the in vitro influence of methisoprinol on the SVCV and virus isolated from catfish (Ictalurus melas) replication by measuring their RNA synthesis. The viruses were propagated in EPC cells and cell cultures containing methisoprinol were followed by infection with SVCV or catfish rhabdovirus suspension containing 107TCID50/ml. Methisoprinol (Polfa, Poland) at concentrations of 0, 100, 200, 300, 400 and 500 μg/ml of medium (Glasgow MEM) was used in this study. The results of this study show the strong inhibition of incorporation (cpm) of [3H]-uridine into SVCV and catfish rhabdovirus RNA in cell culture exposed to methisoprinol at various concentrations. The highest percent of inhibition of viral RNA at 72 h after infection with two rhabdoviruses were observed in doses of 400 and 500 μg/ml of methisoprinol in medium. The results of this in vitro study showed that methisoprinol inhibits the rhabdoviruses isolated from carp and catfish.
19

Parvovirus particles in a fetal-heart with myocarditis: ultrastructural and immunohistochemical study

51%
Respondek M., Bratosiewicz J., Pertynski T., Liberski P. P.
Archivum Immunologiae et Therapiae Experimentalis
|
1997
|
tom 45
|
nr 5-6
20

NTPase-helicase of Flaviviridae: inhibitors and inhibition of the enzyme

51%
Borowski P., Niebuhr A., Schmitz H., Hosmane R. S., Bretner M., Siwecka M. A., Kulikowski T.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 3
RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of pro­teins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are in­volved in transcription and replication of viral single-stranded RNA genomes. The en­zymatic activities of a NTPase/helicase were also detected in the carboxyl-terminal non-structural protein 3 (NS3) of members of the Flaviviridae family. The crucial role of the enzyme for the virus life cycle was demonstrated in knock out experiments and by using NTPase/helicase specific inhibitors. This makes the enzyme an attractive tar­get for development of Flaviviridae-specific antiviral therapies. This review will sum-marize our knowledge about the function and structure of the enzyme, update the spectrum of inhibitors of the enzymatic activities of the NTPase/helicase and describe the different mechanisms by which the compounds act. Some of the compounds reviewed herein could show potential utility as antiviral agents against Flaviviridae viruses.
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