Wyniki wyszukiwania

1

The role of endogenous nitric oxide in inhibition of ischemia-reperfusion-induced cardiomyocyte apoptosis

100%

Czarnowska E., Kurzelewski M., Beresewicz A., Karczmarewicz E.

Folia Histochemica et Cytobiologica

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2001

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tom 39

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nr 2

2

Trehalase as a possible marker of intestinal ischemia - reperfusion injury

86%

Toth,Jr. S., Pekarova T., Varga J., Tomeckova V., Toth S., Lakyova L., Vesela J.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

Background: Different pathological affections of the small intestine cause corresponding morphological and functional changes. The present study was aimed to assess intestinal trehalase activities during ischemia and following reperfusion, correlate them with the pathological changes and determine whether trehalase could be used as a biochemical marker of the intestinal ischemia, ischemia - reperfusion injury. Material and methods: Wistar rats, randomly divided into 5 experimental groups (IR) (each n=15), were subjected to one hour mesenteric ischemia followed by 0, 1, 4, 12 and 24 hours of reperfusion. As a control group sham operated animals were used (n=15). The activity of trehalase was determined using an adapted Dahlqwist method. The range of intestinal injury was determined using histological (histopathological injury index and goblet cell quantification) and immunohistochemical (Ki67, InSitu TUNEL) methods. Results: The highest activities of trehalase were recorded in the control group (C=4.42±0.373 μmol/mg/h). The most altered intestinal histology detected in group IR1 was accompanied by the lowest trehalase activity (IR1=0.97±0.209 μmol/mg/h; p < 0.001 C vs. IR1). Improved histological structure in the remaining reperfusion periods correlated with increase in trehalase activity. Almost normal mucosal histological architecture and 72% of the enzymatic activity were restored after 24 hours of reperfusion (IR24=3.20±0.266 μmol/mg/h; p < 0.01 IR1 vs. IR24). Conclusion: The correlation between intestinal histology and trehalase activities during intestinal injury has been shown. Trehalase activity is closely associated with the status of the histological architecture of the small intestine.

3

Role of oxygen free radicals in cardiocyte injury in the reperfused rat heart

86%

Czarnowska E., Karwatowska-Prokopczuk E.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

4

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Effect of the addition of zinc and selenium ions on the stability of the Biolasol liquid used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity

72%

Ostrozka-Cieslik A., Dolinska B., Caban A., Ryszka F.

Journal of Elementology

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2015

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tom 20

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nr 2

The Biolasol® liquid is an innovative solution used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity. Substances in the liquid prevent cellular oedema and help to maintain a proper water/mineral as well as acid/base balance in the intracellular environment. They also minimize free-radical injuries and ensure the integrity of the cellular membrane structure. The Biolasol® liquid has been shown to be much more efficient than the HTK liquid in the preservation of kidneys. The Biolasol® liquid containing 0.5 mM of vitamin C has been modified by adding ions of Se(IV), Zn(II), and their effect on the stability of the solution was examined. An accelerated aging test was applied to test the liquid stability. The test, based on the laws of chemical kinetics, was conducted at four temperatures at a 10°C step, that is: 50°C±0.05, 60°C±0.05, 70°C±0.05 and 80°C±0.05. The relative humidity equalled 75% of RH and the duartion of the test was 40 days. In order to determine the stability of the tested solutions, the Arrhenius Dependence equation was used, applied to the effect of temperature on the glucose decomposition reaction rate: lnk=lnA-(Ea/RT). The results indicate that the addition of zinc decreases the stability of the liquid by 30.5%, while the addition of selenium prolongs the stability by 8.21%. This is explained by the synergism of action of vitamin C and Se4+ antioxidant in the tested liquid. Zinc ions present in the solution increase the glucose decomposition reaction rate.

5

Effects of 3-aminobenzamide on ultrastructure of hippocampal CA1 layer after global ischemia in gerbils

72%

Strosznajder R. P., Walski M.

Journal of Physiology and Pharmacology. Supplement

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2004

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tom 55

|

nr 3

127-133

Poly(ADP-ribose) polymerase (PARP EC 2.4.2.30) is a key enzyme in the DNA repair machinery, but its excessive stimulation during reperfusion after ischemia could play a critical role in cell death. Our previous study indicated that the PARP inhibitor 3-aminobenzamide (3-AB) significantly protected neuronal cells against death after a short ischemic insult. In this study we investigated the effect of 3-AB on the ischemia-evoked alterations in intracellular organelles. Gerbils were submitted to 3 min of transient forebrain ischemia followed by reinstitution of recirculation for 1-7 days. Electron microscopy showed only the signs of necrotic cell death after ischemia-reperfusion. The examination of specimens revealed a pronounced protective effect of 3-AB on the swelling of astrocytes and neurons 1 day after the ischemic insult. 3-AB also decreased the swelling of pericytes, but it had no effect on the accumulation of osmiophilic inclusions and fibril formation in astrocytes. 3-AB decreased the ischemia-induced swelling of mitochondria. The protective effects of 3-AB on cellular ultrastructure were also observed 7 days after reperfusion. These findings indicate that the inhibition of PARP may have a protective effect on cell swelling and on the state of intracellular organelles after a short-term ischemic episode.

6

Study on ischemic-reperfusion damage to the liver: hitherto existing successes and defeats

72%

Rutkowski T., Plewka A., Kaminski M.

Acta Poloniae Toxicologica

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2000

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tom 08

|

nr 1

7

Arterial ketone index in assessing liver function and its detoxicative capability after ischemia-reperfusion injury

72%

Caban A., Wiaderkiewicz R., Kaminski M., Oczkowicz G., Ziaja J.

Acta Biochimica Polonica

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2000

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tom 47

|

nr 4

Arterial ketone index (AKBR) which is the ratio of acetoacetic acid to 3-hydroxybutyric acid in the arterial blood, is believed to reflect the mitochondrial reduction potential of hepatocytes and general energy state of the liver. In the presented paper we challenged this hypothesis by analysing the correlation between AKBR and the results of typical liver blood tests (AspAT, AlAT, LDH, CRP) and biotransforming potential of the liver (cytochromes P450, b5 and their corresponding NADPH and NADH reductases) in the model of ischemia-reperfusion injury of rat liver. The results were compared with histochemical analysis of distribution and activity of SDH, LDH and G-6-Pase, the key marker enzymes of the liver. We have shown that, except in the case of acute phase protein (CRP), a decrease in AKBR correlated well with the increase of the level of indicator enzymes in serum. Histochemical analysis also confirmed that AKBR correlates with the degree of damage to hepatocytes during early stage of reperfusion after 60 min of liver ischemia. In the Spearman test, AKBR was significantly correlated with the changes in cytochrome P450 content and its NADPH reductase activity which indicates a high sensitivity of this test. We conclude that the decrease of AKBR value reflects the impairment of basic energy pathways and detoxicative capability of the liver.

8

The total antioxidant capacity of blood plasma during cardiovasculary bypass surgery in patients with coronary heart disease

72%

Kedziora-Kornatowska K., Bartosz M., Mussur M., Zaslonka J., Kedziora J., Bartosz G.

Cellular and Molecular Biology Letters

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2003

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tom 08

|

nr 4

We studied the effect of ischemia and reperfusion on the total antioxidant capacity (TAC) of blood plasma during cardiopulmonary bypass surgery employing the modified St. Thomas Hospital cardioplegic solution. TAC was determined using the FRAP method. TAC decreased during surgery, but no further decrease in TAC was observed during reperfusion, indicating that it is a relatively stable parameter of the antioxidative barrier of the body.

9

The role of polymorphonuclear neutrophils in myocardial damage during ischemia and reperfusion

72%

Wysocki H.

Archivum Immunologiae et Therapiae Experimentalis

|

1992

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tom 40

|

nr 1

10

Biphasic enhacement of nitric oxide synthase activity and cGMP level following brain ischemia in gerbils

72%

Strosznajder J., Chalimoniuk M.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

|

nr 1

11

Fucoidan improves the renal blood flow in the early stage of renal ischemia-reperfusion injury in the rat

72%

Bojakowski K., Abramczyk P., Bojakowska M., Zwolinska A., Przybylski J., Gaciong Z.

Journal of Physiology and Pharmacology

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2001

|

tom 52

|

nr 1

12

Energetic state of the postischemic myocardium and its relation to contractile failure

72%

Andres J., Flameng W., Van Belle H.

Journal of Physiology and Pharmacology

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1994

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tom 45

|

nr 1

13

Brain-gut axis in ischemic preconditioning of gastro-duodeno-pancreatic cluster

72%

Konturek S. J., Brzozowski T., Pajdo R., Dembinski A., Jaworek J.

Journal of Physiology and Pharmacology. Supplement

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2001

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tom 52

|

nr 2

14

Ischemia-reperfusion of the liver impairs the inducibility of microsomal cytochrome P450 system by phenobarbital in rats

72%

Wiaderkiewicz R., Wiaderkiewicz A., Czekaj P., Lebda-Wyborny T., Kaszuba M.

Acta Poloniae Toxicologica

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2002

|

tom 10

|

nr 2

15

The effect of oxidative damage on ATP level in heat stressed endothelial cells

72%

Gray C. C., Smolenski R. T., Amrani M., Taylor G., Yacoub M. H.

Cellular and Molecular Biology Letters

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1999

|

tom 04

|

nr 3

16

Na,K-ATPase activity of rabbit kidney cortex membranes in ischemia and reperfusion

72%

Aydin S., Aricioglu A., Turkozkan N., Bingol F., Aydin C.

Acta Biochimica Polonica

|

1993

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tom 40

|

nr 4

The activity of Na+,K*-ATPase in the microsomal fraction of rabbit kidney cortex was strongly decreased by ischemia and increased slightly, but not significantly, after reperfusion. These changes were correlated with a dramatic increase in lipid peroxidation in microsomes isolated from both ischemic and reperfused kidneys. This correlation may point to irreversible impairment of the enzymatic function under the influence of either oxygen free radicals or lipid peroxidation.

17

CGRP as mediator of circulatory and myoelectric changes observed in the small intestine subjected to ischemia and reperfusion [I-R]

72%

Sendur R., Biernat J., Obuchowicz R., Gajda M., Dembinski A., Warzecha Z., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

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2001

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tom 52

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nr 2

18

Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury

72%

Kwiecien S., Brzozowski T., Konturek S. J.

Journal of Physiology and Pharmacology

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2002

|

tom 53

|

nr 1

The exposure of gastric mucosa to damaging factors,such as ethanol,water restraint stress,or ischemia followed by reperfusion,produces pathological changes: inflammatory process,hemorrhagic erosions,even acute ulcers.The base of these changes is a disturbance of protective mechanisms and disrupture of gastric mucosal barrier.Previous studies pointed out the role of disturbances of gastric blood flow,mucus secretion and involvement of prostaglandins and nitric oxide formation in the pathomechanism of gastric mucosa lesions.The role of reactive oxygen species (ROS)in these processes has been little studied.Aim:The purpose of our present investigations is to explain the participation of ROS in acute gastric mucosal damage by various irritants.Material and methods:Experiments were carrying out on 80 male Wistar rats.To assess gastric blood flow (GBF)laser Doppler flowmeter was used.The area of gastric lesions was established by planimetry.The levels of proinflammatory cytokines were measured by ELISA technique.The colorimetric assays were used to determine of malondialdehyde (MDA)and 4-hydroxynonenal (4-HNE)as well as superoxide dismutase (SOD)activity.Results:We demonstrated that 3.5 h of water immersion and restraint stress (WRS),30 min of gastric ischemia followed by 60 min of reperfusion or intragastric administration of 100%ethanol,all resulted in appearance of acute gastric mucosal lesions accompanied by a significant decrease of gastric blood flow. These lesions are also accompanied by the significant increase of proinflammatory cytokines including interleukin –1 beta (IL-1 ß )and tumor necrosis factor alpha (TNF alpha )plasma level. Biological effects of ROS were estimated by measuring tissue level of MDA and 4-HNE, the products of lipid peroxydation by ROS,as well as the activity of SOD,the scavanger of ROS.It was established that 3.5 h of WRS,ischemia-reperfusion and 100%ethanol lead to significant increase of MDA and 4-HNE mucosal level,accompanied by a decrease of SOD activity (significant in WRS and ethanol application).Conclusions:The pathogenesis of experimental mucosal damage in rat stomach includes the generation of ROS that seem to play an important role,namely due to generation of lipid peroxides,accompanied by impairment of antioxidative enzyme activity of cells.

19

Does preconditioning protect against experimental ischaemia-reperfusion injury via cholinergic stimulation?

72%

Dzierzkowska J., Gajewska J., Witanowska A., Deptuch T. W., Kurenko M., Gajewski M., Mautiris J., Kuzma M., Dron T., Maslinski S.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

|

nr 2

20

Alterations of tumor and normal tissue of human lung cancer resection specimens after isolation perfusion

58%

Nowak K., Hanusch C., Kolbel H. C., Schwarzbach M., Post S., Beck G., Gebhard M. M., Metzger R., Hohenberger P.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

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nr 5

Ograniczanie wyników

The role of endogenous nitric oxide in inhibition of ischemia-reperfusion-induced cardiomyocyte apoptosis

Trehalase as a possible marker of intestinal ischemia - reperfusion injury

Role of oxygen free radicals in cardiocyte injury in the reperfused rat heart

Effect of the addition of zinc and selenium ions on the stability of the Biolasol liquid used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity

Effects of 3-aminobenzamide on ultrastructure of hippocampal CA1 layer after global ischemia in gerbils

Study on ischemic-reperfusion damage to the liver: hitherto existing successes and defeats

Arterial ketone index in assessing liver function and its detoxicative capability after ischemia-reperfusion injury

The total antioxidant capacity of blood plasma during cardiovasculary bypass surgery in patients with coronary heart disease

The role of polymorphonuclear neutrophils in myocardial damage during ischemia and reperfusion

Biphasic enhacement of nitric oxide synthase activity and cGMP level following brain ischemia in gerbils

Fucoidan improves the renal blood flow in the early stage of renal ischemia-reperfusion injury in the rat

Energetic state of the postischemic myocardium and its relation to contractile failure

Brain-gut axis in ischemic preconditioning of gastro-duodeno-pancreatic cluster

Ischemia-reperfusion of the liver impairs the inducibility of microsomal cytochrome P450 system by phenobarbital in rats

The effect of oxidative damage on ATP level in heat stressed endothelial cells

Na,K-ATPase activity of rabbit kidney cortex membranes in ischemia and reperfusion

CGRP as mediator of circulatory and myoelectric changes observed in the small intestine subjected to ischemia and reperfusion [I-R]

Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury

Does preconditioning protect against experimental ischaemia-reperfusion injury via cholinergic stimulation?

Alterations of tumor and normal tissue of human lung cancer resection specimens after isolation perfusion