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The characteristic features of guinea pig amygdala (CA), as shown by volumetric comparisons of the individual nuclei, are the poor development of the basolateral (BL) and lateral olfactory tract (NLOT) nuclei as well as the strong formation of the lateral (LA) and basomedial (BM) nuclei. The central (CE), cortical (CO) and medial (ME) nuclei also appear to be well represented in this species. All these features are even more pronounced when the total number of neurons in the nuclei referred to was taken into consideration. A comparison of the densities of neurons in the individual nuclei with the mean numerical density of cells in the guinea pig CA indicates that the densities of neurons in LA, BL, BM, CE and CO are significantly lower than the mean (p < 0.05), whereas in the ME and NLOT these values are significantly higher than the mean (p < 0.05). It is noteworthy, that the densities of the neurons in CE and CO do not differ statistically from each other (p > 0.05) and are significantly higher than the respective values in LA, BL and BM (p < 0.05). Furthermore, a similar division of the guinea pig CA may to some extent be made using the size parameters of the amygdaloid neurons as a marker. Interestingly, the large neurons populate organised CA areas like LA, BL and BM less densely, whereas the small cells create ME and NLOT, where the neurons are densely arranged. CE and CO occupy intermediate positions, with the neurons similar in size to the mean for the guinea pig CA.
The anatomical method with Computer- Digital Image Analysis system served to examine large skin arteries of the crus and the fool in 152 human specimens. Their sources were: 1. popliteal artery (rr: gastrocnemii: medialis - 62.89%, lateralis - 78.95%), 2. anterior tibial artery (n. peronealis laterarlis superior- 97.37%, a. peronealis lateralis inferior - 62.50%, cr. collateralis inferior lateralis - 78.95%), 3. posterior tibial artery (r: circumflexus fibulae - 75.66%, r. soleus lateralis - 48.03%, r. musculi flexoris hallucis longi - 84.87%), 4. peroneal artery (r. perforans - 86.84%) and 5. medial plantar artery (r. profundus - 89.47%). The following ones characterized themselves with the longest course: a. peronealis lateralis superior (112±21 mm), r: gastrocnemius medialis (93±11 mm) and r. soleus lateralis (91±8 mm). The largest flaps supplied successively: r. gastrocnemius medialis (8530±1125 mm²), a. peronealis lateralis superior (7920±1227 mm²), r. profundus a. plantaris medialis (7580±1820 mm²), r. gastrocnemius lateralis (7320±1320 mm²), r. soleus lateralis (7140±1630 mm²) and a. collateralis inferior lateralis (6220 ±1981 mm²).
Every year, the number of anterior cruciate ligament ruptures increases considerably. The present analysis compares patients’ subjective sensations after the ACL injury to an objective arthrometric examination. The subjective evaluation of the knee-joint condition was performed using the Lysholm score scale and IKDC 2000 scale, whereas the objective evaluation was performed using a GNRB device to obtain an arthrometric measurement. Both measurement methods were performed before ACL reconstruction and after the rehabilitation process of ±11.5 months. The following research was conducted on a group of 8 patients who underwent anterior cruciate ligament reconstruction. Statistical analysis did not reveal any significant differences between the arthrometric examination and the subjective score scales. The test indicated the significant differences between the score scales questionnaires completed before and after the surgery. The conclusions that can be drawn from the present analysis show us the importance of using subjective evaluation devices. Despite the lack of significance in the examination performed with the objective device, the self-evaluation of kneejoint efficiency made by the patient indicated a significant improvement.
Tremendous efforts have been made to ameliorate and improve locomotor function after spinal cord injury (SCI) by the transplantation of various types of stem cells. In our study we compared the use of non-neurogenic stem cells – bone marrow stromal cells (MSCs), an immortalized stem cell line (SPCs) derived from human fetal spinal cord tissue or human induced pluripotent stem cell-derived neural precursors (iPS-NPs) – for the treatment of a balloon-induced spinal cord compression lesion. Suspensions of stem cells were implanted into the lesion one week after SCI, while the control groups were injected with saline. Locomotor and sensitivity tests were performed weekly for two months. Animals transplanted with any cell type displayed significant motor and sensory improvement compared to the controls. Morphometric evaluation showed that the white matter was spared in all grafted animals when compared to controls, while the gray matter was spared only in animals implanted with MSCs or iPS-NPs. Two months post-implantation (PI), all types of grafted cells survived in the lesion; however, MSCs, unlike iPS-NPs and SPCs, did not differentiate nor communicate with the host tissue. Compared to SPCs, which partially filled the lesion cavity, iPS-NPs interacted more with the host tissue. Besides differentiating into MAP2-, 5TH- and Dcx-positive neurons, iPS-NPs differentiated into CNPase+ oligodendrocytes. A few cells expressed ChAT, while others were DARPP32+ . SPCs expressed mainly GFAP; however, already at two months PI we found 25% of the cells to be positive for Nkx 6.1, and at four months PI the cells were positive for ChAT and Islet2, motor neuron-specific markers. qPCR revealed the increased expression of rat and human neurotrophin genes as well as human motor neuron-specific genes. Based on staining for GAP43, SPCs cells supported endogenous neurite sprouting and regeneration. Another important therapeutic goal is treating chronic SCI, possibly by a combination of stem cells and bridging scaffolds. Hydrogel bridges seeded with MSCs were implanted into SCI one month after injury. The implanted rats were behaviorally tested, then sacrificed 6 months PI and the spinal cord lesions histologically evaluated. The hydrogels adhered well to the surrounding tissue and completely filled the post-traumatic cavity. MSCs survived in the hydrogel, and neurofilaments, blood vessels and Schwann cells infiltrated the implant. Combined therapy also prevented tissue atrophy, while behavioral analysis showed an improvement in rats with combined treatment, compared with the control group. Our results demonstrate that the transplantation of neurogenic as well as non-neurogenic stem cells into the lesioned rat spinal cord improves functional outcome by providing trophic support to the spared axons in the injured tissue. Neurogenic stem cells have the ability to interact with the host tissue and differentiate into a more mature phenotype, such as motor neurons. Treatment of chronic spinal cord injury will require a combination of cell therapy and lesion bridging. Supported by IAA500390902, GA CR: P304/12/1370, GA CR: P108/10/1560.
With the emerging utilisation of ansa cervicalis in nerve reconstructive surgery, it is important for surgeons to be conversant with the anatomy of these nerves. This descriptive cross sectional study aimed at describing the morphology and topographic anatomy of ansa cervicalis. We examined 38 adult human formalin-fixed cadavers. The superior root was present in 38 (100%) cases and 37 (97%) cases, on the right and left sides, respectively. More than half (56%) of these roots were located superior to the posterior belly of the digastric muscle. The inferior root, on the other hand, was present in 34 (89.5%) cases on the right side and 31 (81.6%) cases on the left side. Of all the inferior roots, 81.5% were located lateral to the internal jugular vein. The loop was seen in all the cases that had the inferior root, and was mostly (64.6%) located above the superior belly of the omohyoid muscle. Knowledge of the anatomy of ansa cervicalis is not only important for nerve reconstruction surgeries, but also for operations in the neck, so as to avoid injuring the great vessels that are closely related to it. (Folia Morphol 2010; 69, 3: 160–163)
A survey of fully-sequenced chloroplast genomes revealed that in land plants there are six tRNA genes that have introns. Moreover, the length of a particular tRNA gene intron remains relatively stable across species. However, in algae, the presence of chloroplast tRNA genes containing introns is exceptional. A survey of mitochondrial plant genomes revealed intron-containing tRNA genes are rather rare features, with the exception of tRNASerGCU genes in liverworts and peat-mosses. We isolated and sequenced one mitochondrial and three chloroplast intron-containing tRNA genes and a fragment of the mitochondrial coxIII gene containing the first intron from the following liverwort species: Pellia borealis, Pellia epiphylla-species N, Pellia epiphylla-species S and Porella baueri, Porella cordaeana, Porella platyphylla. We showed that, as in the case of higher plants, the rate of nucleotide substitution is lower in the mitochondrial genome than in the chloroplast genome. Moreover, the comparison of intron nucleotide sequences enabled us to show that in the case of one allopolyploid species, Pellia borealis, organelles were transmitted from one parent species, Pellia epiphylla-species N. In the case of another allopolyploid species, Porella baueri, organelles were also inherited from one parent species, Porella cordaeana. Therefore, organellar inheritance in liverworts seems to be uniparental. It remains clear that analysis of carefully chosen chloroplast and mitochondrial DNA sequences allowed us to reconstruct historical events.
The long bones (humerus, radius, metacarpus, femur, tibia, metatarsus) of 51 extant bovid and 7 equid specimens were measured in order to test the hypothesis that they show adaptations to different habitats. We performed factor analyses (FAs) with principal component extraction method and plotted the extracted factors (Fs) in simple scatterplots. The preferred habitats (grassland, forest, mountainous regions) were labeled in the plots, and our results show three clearly separated clusters for F2 vs. F3. According to our interpretation, F1 reflects the body size of the specimens while F2 is most probably reflecting cursorial adaptations. F3 is largely affected by dimensional bone characteristics adapted to maneuver in the environment, and therefore, F3 is somehow linked to habitat. The investigated equids are plotting within the cluster of bovids preferring grassland habitats, which is surprising because of different constructions of the metapodials in perissodactyls and ruminants. Performed linear discriminant analyses (LDAs) are supporting our FA results. This approach combines biometrics with statistics and presents a tool, which easily can be applied helping to identify the paleo-habitat or the paleo-ecology of extinct bovids with implications on fossil localities.
Age changes of bone tissue and molar cementum, and formation of daily layers in dentin of incisors enable us to estimate age and maximum lifespan, season of birth, season of death, sexual maturation, sex in adults, and in combination with tetracycline prebaiting, movements and space distribution of rodents. In hibernating rodents, the hibernation zone formed in incisor dentin can be used to assess inter- and intra­population variations in seasonal activity and growth. This approach is illustrated by examples from the study of rodents of the genera Apodemus and Sicista.
Fossil utricular otoliths (= lapilli) from cypriniform fishes have long been recorded from European Oligocene and Neogene freshwater and oligohaline sediments. Until now, their determination was limited to the family level owing to the lack of morphological investigations on lapilli of Recent cypriniforms. The present study introduces a terminology for the lapillus morphology that is based on the lapilli of 134 specimens of 20 cyprinid and one balitorid species. It is demonstrated that the lapillus has valuable characters for taxonomic classification. As a result, fossil lapilli from Oligocene and Miocene continental deposits from the western Mediterranean, the Swiss and the South German Molasse Basin, the Mainz Basin, and additionally from Anatolia could be determined. Nine species were identified: aff. Abramis sp. vel aff. Alburnus sp., aff. Alburnoides sp., aff. Barbus sp., cf. Leuciscussp., Palaeoleuciscus sp., Palaeotinca moeddeni sp. nov., Palaeotinca sp. 1, aff. Phoxinus sp., and aff. Rutilus sp. vel aff. Scardinius sp. Our study includes the oldest record of a Phoxinus−related and a Palaeotinca species from Europe. Additionally, aff. Abramis sp. vel aff. Alburnus sp. and aff. Alburnoides have been identified as fossils for the first time. The determination of the fossil lapilli has been supported by means of pharyngeal teeth, with the exception of aff. Abramis sp. vel aff. Alburnus sp., whose lapilli were found together with pharyngeal teeth of Palaeocarassius sp. It is suggested that these so−called Palaeocarassius pharyngeal teeth do not belong to an ancestor of the Carassiuslineage, but to a forerunner of the Abramis or Alburnuslineage. Our results support the previously described turnover in the Paratethys freshwater fish fauna about 17–18 Ma ago, when Palaeotinca spp. became extinct and the first appearance of Palaeoleuciscussp. and Palaeocarassiussp. (= aff. Abramissp. vel aff. Alburnus sp.) occurred. The Oligocene and Miocene cypriniform fishes did not evolve any provincialism from southern France throughout the Molasse Basin to the Mainz Basin.
Here we present the first attempt to use the BovineSNP50 Illumina Genotyping BeadChip for genome-wide screening of European bison Bison bonasus bonasus (EB), two subspecies of American bison: the plains bison Bison bison bison (PB), the wood bison Bison bison athabascae (WB) and seven cattle Bos taurus breeds. Our aims were to (1) reconstruct their evolutionary relationships, (2) detect any genetic signature of past bottlenecks and to quantify the consequences of bottlenecks on the genetic distances amongst bison subspecies and cattle, and (3) detect loci under positive or stabilizing selection. A Bayesian clustering procedure (STRUCTURE) detected ten genetically distinct clusters, with separation among all seven cattle breeds and European and American bison, but no separation between plain and wood bison. A linkage disequilibrium based program (LDNE) was used to estimate the effective population size (N e) for the cattle breeds; N e was generally low, relative to the census size of the breeds (cattle breeds: mean N e = 299.5, min N e = 18.1, max N e = 755.0). BOTTLENECK 1.2 detected signs of population bottlenecks in EB, PB and WB populations (sign test and standardized sign test: p = 0.0001). Evidence for loci under selection was found in cattle but not in bison. All extant wild populations of bison have shown to have survived severe bottlenecks, which has likely had large effects on genetic diversity within and differentiation among groups.
The president of Byelorussia undertook in 2008 certain measures of state support for the agricultural enterprises, in particular for organizing dairy farms. These measures concerned conditions of crediting the enterprises and they influenced the final efficiency of investment projects. A case study with calculation of an economic efficiency of organizing a dairy farm in Grodno region is analysed.
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