Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: recombinant strain

Sortuj według:

Ogranicz wyniki do:

Recombinant strains of Escherichia coli for L-aspartic acid biosynthesis

100%

Gadomska G., Plucienniczak A., Chmiel A.

Polish Journal of Microbiology

2007

tom 56

nr 2

The aspartase overproducing mutant B-715 was used as a donor of the aspartase gene for further construction of the aspartase-hyperproducing strains by molecular cloning. In preliminary experiments activity of transformants and their efficiency in L-aspartic acid biosynthesis were compared. The conditions for recombinant strain multiplication, biomass activation and L-aspartic acid biosynthesis were optimized. The optimum temperature for cells multiplication, their activation and for product biosynthesis was 37°C. Two-stage process of the multiplication of bacteria (first in LB medium, and then in FF medium) eliminates the appearing of the inclusion bodies of aspartase in the cells. The shaking during cell activation improved cells productivity. The change of pH in the course of the biosynthesis process was insignificant but did not influence the process.

Analysis of genetic markers Svp-1 and Svp-3 in recombinant mouse strains CBXE and EXCB

84%

Styrna J., Miksztal T., Rypel-Chrzan A.

Folia Biologica

1994

tom 42

nr 3-4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Identifying overlapping phylogenetic and geographic roots of HIV - 1 evolution through computational analyses

67%

Singh P. K., Banik R., Chakraborty H. J., Das S. G., Ganguli S., Datta A.

International Letters of Natural Sciences

2014

tom 02

HIV-1 or Human Immuno Deficiency Virus-1 is the main causative agent of Acquired Immuno Deficiency Syndrome (AIDS). Human host infected with HIV - 1 extensively harbours many viral variants but very little is known about the difference in pattern[17] of evolution of phylogenetic lineages of HIV-1 non recombinant, normal inter subtype recombinant and main two specific recombinant forms of HIV-1 i.e., Circulating Recombinant Forms (CRFs) and Unique Recombinant Forms (URFs). This study is mainly concerned with study of the difference in evolutionary lineages of non-recombinant and recombinant sequences of HIV-1 genome sequences and identification of geographically rich areas which has reported high degree of HIV-1 occurrence and variety. Total 1550 HIV-1 genome sequences were obtained from HIV Los Alamos Database. The sequences were aligned using MAFFT (Multiple Alignment using Fast Fourier Transform) web server tool. Alignment was carried out using 10 different set of alignment parameter values. After alignment the aligned file was used for constructing N-J phylogenetic tree using Clustal X2 tool. Phylogenetic analysis was performed keeping in mind the category to which the sequence belongs. Upon analysis it was observed that the clade containing the probable ancestor belongs remained constant in all cases of different alignment values. Non recombinant isolates, inter subtype recombinants, CRFs, URFs all followed different patterns of evolution. Non recombinant sequences were found geographically specific and subtype specific to some extent whereas, normal recombinants were subtype specific and less geographically specific. CRFs showed variation among the pattern of their evolution. At some instances the sequences occurred as sister taxa of non-recombinant or normal inter subtype recombinant sequences, while at some instances as sister taxa of other CRFs where they were geographically specific. Three CRFs existed as completely diverged sequences. URFs were four in number; two of them were Indian isolates of while other two were Japanese isolates. URFs were found to be totally geographically specific. Geography wise high rate of variation was observed in India and Japan as these two countries had sequences belonging to all of the above categories. Cameroon and South Africa have very large number isolates and a considerable amount of genetic variation among isolates but they lack URFs.

Ograniczanie wyników

1 Folia Biologica

1 International Letters of Natural Sciences

1 Polish Journal of Microbiology

1 Banik R.

1 Chakraborty H. J.

1 Chmiel A.

1 Das S. G.

1 Datta A.

1 Gadomska G.

1 Ganguli S.

1 Miksztal T.

1 Plucienniczak A.

1 Rypel-Chrzan A.

1 Singh P. K.

1 Styrna J.

1 2014

1 2007

1 1994

Wyniki wyszukiwania

Recombinant strains of Escherichia coli for L-aspartic acid biosynthesis

Analysis of genetic markers Svp-1 and Svp-3 in recombinant mouse strains CBXE and EXCB

Identifying overlapping phylogenetic and geographic roots of HIV - 1 evolution through computational analyses