Wyniki wyszukiwania

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The identification of QTLs associated with the in vitro response of rye [Secale cereale L.]

100%

Bolibok H., Gruszczynska A., Hromada-Judycka A., Rakoczy-Trojanowska M.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 4

523-535

This study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated: callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R.

2

Effect of nutrient deprivation stress on seedlings morphology and ROS formation in selected RILs of rye (Secale cereale L.)

84%

Smolik M., Cieluch P., Mazurkiewicz-Zapalowicz K.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2013

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tom 16

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nr 2

3

Extension of the Messapia x dicoccoides linkage map of Triticum turgidum [L.] Thell.

84%

Blanco A., Simeone R., Cenci A., Gadaleta A., Tanzarella O. A., Porceddu E., Salvi S., Tuberosa R., Figliuolo G., Spagnoletti P., Roder M. S., Korzun V.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 3

A set of recombinant inbred lines (RIL) derived from a cross between the cultivar Messapia of durum wheat (Triticum turgidum var. durum) and the accession MG4343 of T. turgidum var. dicoccoides was analysed to increase the number of assigned markers and the resolution of the previously constructed genetic linkage map. An updated map of the durum wheat genome consisting of 458 loci was constructed. These loci include 261 Restriction Fragment Length Polymorphisms (RFLPs), 91 microsatellites (Simple Sequence Repeats, SSRs), 87 Amplified Fragment Length Polymorphisms (AFLPs), two ribosomal genes, and nine biochemical (seven seed storage proteins and two isozymes) and eight morphological markers. The loci were mapped on all 14 chromosomes of the A and B genomes, and covered a total distance of 3038.4 cM with an average distance of 6.7 cM between adjacent markers. The molecular markers were evenly distributed between the A and the B genomes (240 and 218 markers, respectively). An additional forty loci (8.8%) could not be assigned to a specific linkage group. A fraction (16.4%) of the markers significantly deviated from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on the 1B, 2A, 2B, 3A, 4A, 7A and 7B chromosomes. The genetic lengths of the chromosomes range from 148.8 cM (chromosome 6B) to 318.0 cM (chromosome 2B) and approximately concur with their physical lengths. Chromosome 2B has the largest number of markers (47), while the chromosomes with the fewest markers are 3A and 6B (23). There are two gaps larger than 40 cM on chromosomes 2A and 3B. The durum wheat map was compared with the published maps of bread and durum wheats; the order of most common RFLP and SSR markers on the 14 chromosomes of the A and B genomes were nearly identical. A core-map can be extracted from the highdensity Messapia x dicoccoides map and a subset of uniformly distributed markers can be used to detect and map quantitative trait loci.

4

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Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape (Brassica napus L.)

67%

Cegielska-Taras T., Szala L., Matuszczak M., Babula-Skowronska D., Mikolajczyk K., Poplawska W., Sosnowska K., Hernacki B., Olejnik A., Bartkowiak-Broda I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

5

Genetic mapping of polyphenol oxidase in tetraploid wheat

67%

Simeone R., Pasqualone A., Clodoveo M. L., Blanco A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2B

Pasta colour is one of the main factors inﬂuencing pasta quality. It is the product of a desirable yellow component, an undesirable brown component and, under some drying conditions, a red component. The brown colour depends on enzymatic and chemical factors. Polyphenol oxidase (PPO; E.C. 1.14.18.1) is one of the enzymatic factors. It is mainly localised in the peripheral part of the wheat kernel, and is involved in the oxidation of endogenous wheat phenolic compounds resulting in the production of highly coloured products. Therefore, a knowledge of the genetic control of PPO activity could enable the developing of better strategies in breeding programs to reduce pasta darkening. The aim of this study was to map the gene(s) affecting PPO activity using a set of recombinant inbred (RI) lines, derived from a cross between Triticum turgidum L. var. durum cultivar Messapia and the accession MG4343 of Triticum turgidum L. var. dicoccoides. After performing linkage analysis, the gene for high PPO activity was mapped on the long arm of the chromosome 2A and its characteristic was found highly associated to the RFLP marker Xutv1427-2A, with a value of LOD equal to 29.84. The identification of molecular markers linked to loci controlling the PPO activity may potentially accelerate wheat breeding since the selection of plants can be carried out by genotype rather than phenotype.

6

Genetic mapping of the gene affecting polyphenol oxidase activity in tetraploid durum wheat

67%

Watanabe N., Masum A. A. S. M. G., Nachit M. M.

Journal of Applied Genetics

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2006

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tom 47

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nr 3

The quality of durum wheat (Triticum turgidum ssp. durum) is influenced by polyphenol oxidase (PPO) activity and its corresponding substrates. A saturated molecular-marker linkage map was constructed previously by using a set of recombinant inbred (RI) lines, derived from a cross between durum wheat cultivars Jennah Khetifa and Cham 1. Quantitative trait loci (QTL) for PPO activity in seeds were mapped in this population. PPO activity in seeds of the parents and 110 RI lines was measured spectrophotometrically. The PPO activity of Cham 1 was significantly lower than that of Jennah Khetifa. QTL analysis of these data indicated that most of PPO activity was associated with major loci on the long arm of chromosome 2A. The trait was found to be strongly associated with the SSR marker Xgwm312@2A. With this knowledge, marker-assisted selection can be used to select genotypes with lower PPO activity in durum wheat populations.

7

Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin [Lupinus angustifolius L.]

67%

Boersma J. G., Pallotta M., Li C., Buirchell B. J., Sivasithamparam K., Yang H.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 2

8

Genetic control and heritability of resistance to grain mold in F 2:5 sorghum families with non-pigmented testa

67%

Rodriguez-Herrera R., Rooney W. L., Rosenow D. T., Aguilar-Gonzales C. N., Quero-Carrillo A. R.

Plant Breeding and Seed Science

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2007

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tom 55

Ograniczanie wyników

The identification of QTLs associated with the in vitro response of rye [Secale cereale L.]

Effect of nutrient deprivation stress on seedlings morphology and ROS formation in selected RILs of rye (Secale cereale L.)

Extension of the Messapia x dicoccoides linkage map of Triticum turgidum [L.] Thell.

Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape (Brassica napus L.)

Genetic mapping of polyphenol oxidase in tetraploid wheat

Genetic mapping of the gene affecting polyphenol oxidase activity in tetraploid durum wheat

Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin [Lupinus angustifolius L.]

Genetic control and heritability of resistance to grain mold in F 2:5 sorghum families with non-pigmented testa