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The manuscript presents results of investigations aimed at determining changes in conductance properties of raw milk homogenized at pressures of 5, 10, 15 and 20 MPa. Results obtained demonstrated that values of impedance were the highest and those of admittance – the lowest at the applied pressure of ca. 15 MPa, at which additionally the degree of fat globules disintegration appeared to approximate the maximum one (obtained at a pressure of 20 MPa). It was also concluded that the results achieved in the study constitute a basis for further research into the application of electric properties of milk in determination of the degree of fat globules dispersion in the homogenization process of raw milk.
The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this purpose, 100 samples of raw milk were collected and 100 samples of herby cheese sold for consumption in Van province in Turkey were obtained from grocers and markets in order to detect the presence of Escherichia coli O157 and O157:H7. The method of E. coli O157 and O157:H7 isolation proposed by the Food and Drug Administration (FDA) was used. E. coli O157 in raw milk and herby cheese samples was found in 11% and 6% of samples respectively, and E. coli O157:H7 was found in 2% of herby cheese samples. No E. coli O157:H7 was detected in raw milk samples. This study showed that raw milk was contaminated with E. coli O157 and herby cheese was contaminated with both E. coli Ol 57 and E. coli Ol57:H7; therefore, herby cheese poses a serious risk to public health.
The aim of this study was to analyse the association between factors characterising dairy herd management and the levels of somatic cell count (SCC), and total microorganisms count (TMC) in bulk tank milk. The investigations were carried out in 2005 in 187 family farms (herd size from 6 to 60 cows), situated in adjacent parts of Warmia & Mazury, and Mazowsze regions. Based on annual geometric means, all the farms were divided into those with low and elevated SCC level (<200,000 vs. 200,000–400,000 cells/mL), and with low and elevated TMC level in milk (<50,000 vs. 50,000–100,000 cfu/mL). The milk was characterised by low SCC level in 55 farms (29.4%), whereas TMC level was low in 65 farms (34.8%). The following factors were associated with the low level of SCC: small herd size, tie-stall system of cow housing, grazing cows, use of advisory service, use of antibiotics at cow dry-off, dry storing of milking clusters between milkings, post-milking teat dipping, regular use of California Mastitis Test, treatment of clinical cases of mastitis with antibiotics in selected cows only, use of whole straw in cow feeding, and use of salt-licks containing microminerals. Factors associated with the low level of TMC were: large herd size, colostrum feeding to the newborn calf by bucket, individual housing of replacement heifers older than 3 months, pipe-line system of milking, dry storing of milking clusters between milkings, pre-milking udder and teat cleaning with wet towel soaked with a disinfectant, tank system of milk cooling and storage after milking, and regular calculation of feeding rations for cows.
Raw milk from 15 districts in Lithuania was analysed in winter and grazing seasons in 2005. Lead and cadmium were determined by inductively coupled plasma sector field mass spectrometry. Mean cadmium concentrations in winter milk samples amounted to 0.37 μg/kg (0.25-0.49 μg/kg) and in summer ones 0.18 μg/kg (0.11-0.23 μg/kg). The mean concentrations of lead in samples collected in winter and in summer were 0.47 μg/kg (0.17-1.0 μg/kg) and 0.54 μg/kg (0.06-1.76 μg/kg), respectively. No individual sample exceeded the Lithuanian norm value for lead (20 μg/kg). The health quality of Lithuanian milk can be considered as very high in the aspect of cadmium and lead contamination.
The main aim of this study was to evaluate the effect of the lactation stage (SL) on the fatty acids (FA) profile of raw milk of Brown Short-haired goats reared under organic regime. An integraf part of the study was also to assess the effect of the SL on the physico-chemical characteristics and somatic cell count (SCC) of milk. Milk records and samplings of each goat were carried out seven times from April to October. SL had a significant effect on contents of almost all monitored physicochemical properties, somatic cell counts (SCC) and FA of milk and also on average daily milk yield (DMY). DMY gradually decreased with advanced lactation (from 3.44 to 1.44 litre), whereas the content of total solids (TS) increased in the course of lactation (from 10.9 to 14.0%). Also the content of milk fat (F) increased in the course of lactation (from 3.2 to 4.7%). Contents of total protein (TP) and casein (C) were relatively high in early lactation, decreased as lactation peaked and increased towards to late lactation (3.7% of TP and 2.6% of C). Titratable acidity (TA) gradually increased from 90th day (6.2°SH) to the end of lactation (8.5°SH), while positive correlations with TS, F, TP,C and lactose were found. SCC increased as lactation advanced, moreover, a positive correlation among SCC and TA and TS was found. The SL had a significant effect on all FA groups. PUFA gradually decreased towards to late lactation, which was caused mainly by the content of linoleic acid. Similarly, the presence of linolenic acid and PUFA/SFA ratio showed a decreasing tendency with advanced lactation. Also the CLA content was the lowest at the end of lactation. PUFA n-6/n-3 ratio increased in mid lactation (7.8) and hereafter decreased towards to late lactation (3.5). In our opinion, a less favourable presence of particular groups of FA in late lactation was related with a decrease in pasture quality under organic conditions.
The aim of the present study was to determine the effects of cold storage (temp. 4 and 8°C) on some physicochemical properties and technological suitability of raw milk subjected to vertical vibration at various frequencies (10, 30 and 60 Hz). An analysis of the results showed that vibration increased milk acidity and negatively affected milk components. The process caused, among others, an increase in the levels of ionic calcium and free fatty acids, as well as protein degradation (an increase in the formalin number), which deteriorated the technological quality of milk (a decrease in thermal stability and ethanol stability, a shorter rennet coagulation time). The degree of these changes corresponded to an increase in vibrational frequency. Intensive lipolytic and proteolytic processes and enhanced acidity were observed during cold storage of raw milk subjected to vibration. This had a negative effect on the technological suitability of milk. The changes were less significant in the case of deep chilling (4°C).
The following parameters of bulk raw milk, destined for processing were determined: percentage content of fat, protein, milk solids, quantity of fat and protein units and quality class of each batch of raw milk according to PN. The analysis included the results of monthly tests of raw milk for 2002. The mean values of chemical components of bulk milk and of the calculated indices of technological suitability of raw milk: for butter and cheese production, were estimated. The obtained results allowed determination of an average chemical composition of raw milk, specific of the central Poland and its technological suitability, depending on feeding season, size of deliveries and quality class of the purchased raw milk. The co-relationships between the examined properties were determined.
Six cows suffering from mastitis were injected intramuscularly with tylosin at a dose of 10 mg kg⁻¹ b.w. for five consecutive days after the morning milking. The milk samples were collected for 13 days at 12 h intervals, and from the afternoon and morning milkings were pooled together (1:1). The samples were analysed by liquid chromatography and yoghurt inhibitor test for a detection of tylosin residues. Qualitative parameters of milk (active acidity, somatic cell count, total bacterial count) were also investigated. It was found that the HPLC method and yoghurt inhibitor test gave comparable results. The somatic cell count was above 400 000 per ml of milk. Staphylococcus aureus and pathogenic streptococci were detected in milk from mastitic cows.
The content of 38 microelements and trace elements in raw milk of cows from Lower Silesia (LS) and Upper Silesia (US) were examined. Analytical method of mass spectrometry (ICP-MS) with microwave sample digestion was used in this study. Statistically significant differences were detected in the case of iodine, rubidium, cesium, tungsten and thorium (more in the US region) as well as aluminum, titanium, manganese, gallium, selenium, germanium, cobalt (more in LS). No statistically significant differences were observed in the case of: Zn, Ba, Cu, Cr, V, Ni, As, Mo, Pt, Sb, Au, Hf, Ce, U, Re, Tl, Ru, Rh, Ir, Ta and Be. For La, Nb, Ag, In and Y statistical differences were not calculated since in the majority of samples concentrations were below detection limit.
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